Mechanics of a mosquito bite with applications to microneedle design

The mechanics of a fascicle insertion into the skin by a mosquito of the type aedes aegypti has been studied experimentally using high-speed video (HSV) imaging, and analytically using a mathematical model. The fascicle is a polymeric microneedle composed of a ductile material, chitin. It has been proposed that the mosquito applies a non-conservative follower force component in addition to the Euler compressive load in order to prevent buckling and penetrate the skin. In addition, the protective sheath surrounding the fascicle (labium) provides lateral support during insertion. The mechanics model presented approximates the fascicle as a slender column supported on an elastic foundation (labium) subjected to non-conservative (Beck) and conservative Euler loads simultaneously at the end. Results show that the lateral support of the fascicle provided by the labium is essential for successful penetration by increasing the critical buckling load by a factor of 5. The non-conservative follower force application increases the buckling load by an additional 20% and may or may not be necessary for successful penetration. Experimental results showing the importance of the labium have been cited to validate the model predictions, in addition to the video observations presented in this work. This understanding may be useful in designing painless needle insertion systems as opposed to miniaturized hypodermic needles.

[1]  B. Chichkov,et al.  Two photon induced polymerization of organic-inorganic hybrid biomaterials for microstructured medical devices. , 2006, Acta biomaterialia.

[2]  V. Swaminathan,et al.  Mechanics of a Mosquito Bite , 2006 .

[3]  S. Timoshenko.  Theory of Elastic Stability , 1936 .

[4]  Eiji Nakamachi,et al.  Development of Blood Extraction System for Health Monitoring System , 2004, SPIE Micro + Nano Materials, Devices, and Applications.

[5]  Manhee Han,et al.  A novel fabrication process for out-of-plane microneedle sheets of biocompatible polymer , 2007 .

[6]  Yukio Inoue,et al.  CHARACTERISTICS OF CHITIN FOR ORTHOPEDIC USE11Presentation at the Second World Congress on Biomaterials, 10th Annual Meeting of the Society for Biomaterials, Washington, D.C., April 27-May 1, 1984. , 1984 .

[7]  Wijaya Martanto,et al.  Microinfusion Using Hollow Microneedles , 2006, Pharmaceutical Research.

[8]  M. Allen,et al.  Micromachined needles for the transdermal delivery of drugs , 1998, Proceedings MEMS 98. IEEE. Eleventh Annual International Workshop on Micro Electro Mechanical Systems. An Investigation of Micro Structures, Sensors, Actuators, Machines and Systems (Cat. No.98CH36176.

[9]  PHILIP KASHIN,et al.  An Insect ‘Bitometer’ , 1965, Nature.

[10]  G. Venkateswara Rao,et al.  Revisit to the stability of a uniform cantilever column subjected to Euler and Beck loads-Effect of realistic follower forces , 2001 .

[11]  F. H. Jackson,et al.  Analytical Methods in Vibrations , 1967 .

[12]  K. Nakamae,et al.  Elastic modulus of the crystalline regions of chitin and chitosan , 1999 .

[13]  Madoka Takai,et al.  Healthcare Chip for Checking Health Condition from Analysis of Trace Blood Collected by Painless Needle , 2003 .

[14]  Philip Kashin,et al.  Electronic recording of the mosquito bite , 1966 .

[15]  M. Mogi,et al.  Biology of mosquitoes. , 1987 .

[16]  Mark G. Allen,et al.  Polymer Microneedles for Controlled-Release Drug Delivery , 2006, Pharmaceutical Research.

[17]  Susumu Sugiyama,et al.  Geometrical strengthening and tip-sharpening of a microneedle array fabricated by X-ray lithography , 2006 .

[18]  Hiroyuki Fujita,et al.  Fabrication of a Micro Needle for a Trace Blood Test , 2002 .

[19]  Göran Stemme,et al.  Novel Microneedle Patches for Active Insulin Delivery are Efficient in Maintaining Glycaemic Control: An Initial Comparison with Subcutaneous Administration , 2007, Pharmaceutical Research.

[20]  Mark R Prausnitz,et al.  Coated microneedles for transdermal delivery. , 2007, Journal of controlled release : official journal of the Controlled Release Society.

[21]  Mark R Prausnitz,et al.  Insertion of microneedles into skin: measurement and prediction of insertion force and needle fracture force. , 2004, Journal of biomechanics.

[22]  R. Gordon,et al.  An apparatus which enables the process of feeding by mosquitoes to be observed in the tissues of a live rodent; together with an account of the ejection of saliva and its significance in Malaria. , 1952, Annals of tropical medicine and parasitology.

[23]  Mark G. Allen,et al.  Microfabricated needles for transdermal delivery of macromolecules and nanoparticles: Fabrication methods and transport studies , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[24]  G. Venkateswara Rao,et al.  TECHNICAL NOTE: A smart structures concept for the buckling load enhancement of columns , 2001 .

[25]  A. Mioduchowski,et al.  Flow-induced flutter instability of cantilever carbon nanotubes , 2006 .

[26]  A. Wan,et al.  Hydroxyapatite modified chitin as potential hard tissue substitute material. , 1997, Journal of biomedical materials research.

[27]  Z. Celep,et al.  On the Vibration and Stability of Beck's Column Subjected to Vertical and Follower Forces , 1977 .

[28]  J. Vincent,et al.  Design and mechanical properties of insect cuticle. , 2004, Arthropod structure & development.

[29]  R. Rosenfeld.  Nature , 2009, Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery.

[30]  M Shirkhanzadeh,et al.  Microneedles coated with porous calcium phosphate ceramics: Effective vehicles for transdermal delivery of solid trehalose , 2005, Journal of materials science. Materials in medicine.

[31]  D. Liepmann,et al.  A microneedle-based glucose monitor: fabricated on a wafer-level using in-device enzyme immobilization , 2003, TRANSDUCERS '03. 12th International Conference on Solid-State Sensors, Actuators and Microsystems. Digest of Technical Papers (Cat. No.03TH8664).

[32]  J C Jones,et al.  Blood-feeding behavior of adult Aedes aegypti mosquitoes. , 1973, The Biological bulletin.

[33]  A. Spielman,et al.  Role of mosquito saliva in blood vessel location. , 1984, The Journal of experimental biology.

[34]  Max Beck,et al.  Die Knicklast des einseitig eingespannten, tangential gedrückten Stabes , 1952 .

[35]  G. Holzapfel,et al.  Penetration-Enhanced Ultrasharp Microneedles and Prediction on Skin Interaction for Efficient Transdermal Drug Delivery , 2007, Journal of Microelectromechanical Systems.

[36]  L. Meirovitch.  Analytical Methods in Vibrations , 1967 .

[37]  Michael L. Reed,et al.  The evolution from convex corner undercut towards microneedle formation: theory and experimental verification , 2006 .

[38]  R. Hibbeler.  Statics and Mechanics of Materials , 1995 .

[39]  Hans Irschik,et al.  Displacement compensation of beam vibrations caused by rigid-body motions , 2005 .

[40]  Karan V. I. S. Kaler,et al.  Electronic Mosquito: designing a semi-invasive Microsystem for blood sampling, analysis and drug delivery applications , 2005 .

[41]  Jeffrey D Zahn,et al.  Microneedle Insertion Force Reduction Using Vibratory Actuation , 2004, Biomedical microdevices.

[42]  J C Jones.  The feeding behavior of mosquitoes [Aedes aegypti]. , 1978 .

[43]  Eiji Nakamachi,et al.  Development of Blood Extraction System for Health Monitoring System , 2005 .

[44]  T L Daniel,et al.  Feeding strategy and the mechanics of blood sucking in insects. , 1983, Journal of theoretical biology.