SAMl , the Structural Gene for One of the S-Adenosylmethionine Synthetases in Saccharomyces cerevisiae

Saccharomyces cerevisiae contains two genes, SAMl and SAM2, encoding functional S-adenosylmethionine synthetases. The gene SAMl was isolated by functional complementation of a double mutant of S. cerevisiae, and its identity was confirmed by gene disruption. The cloned gene was used to probe wild type chromosomal DNA, and two regions hybridizing with SAMl were found, one of which is the SAMl region. The DNA sequence of SAMl is reported. The translation product shows a high homology with the one deduced from the sequence of the MetK gene encoding the SAM synthetase of Escherichia coli.