Culture and transfer of human blastocysts.

The transfer of the human embryo at the blastocyst stage during an in-vitro fertilization procedure is a way of increasing implantation rates. This, in turn, means that significantly fewer embryos are required to be transferred in order to establish a successful pregnancy. The result of this is that high order multiple gestations are eliminated, while maintaining high pregnancy rates, in in-vitro fertilization.

[1]  E. Baracat,et al.  High implantation and pregnancy rates with transfer of human blastocysts developed in preimplantation stage one and blastocyst media. , 1998, Fertility and sterility.

[2]  J. Thomson,et al.  Embryonic stem cell lines derived from human blastocysts. , 1998, Science.

[3]  D. Gardner,et al.  Culture and selection of viable blastocysts: a feasible proposition for human IVF? , 1997, Human reproduction update.

[4]  B. Bavister,et al.  The neglected human blastocyst revisited. , 1997, Human reproduction.

[5]  A. Campana,et al.  Blastocyst development from supernumerary embryos after intracytoplasmic sperm injection: a paternal influence? , 1998, Human reproduction.

[6]  Y Hu,et al.  Maximizing pregnancy rates and limiting higher-order multiple conceptions by determining the optimal number of embryos to transfer based on quality. , 1998, Fertility and sterility.

[7]  D. Gardner,et al.  Changes in requirements and utilization of nutrients during mammalian preimplantation embryo development and their significance in embryo culture. , 1998, Theriogenology.

[8]  D. Gardner,et al.  Amino acids and vitamins prevent culture-induced metabolic perturbations and associated loss of viability of mouse blastocysts. , 1998, Human reproduction.

[9]  D. Gardner,et al.  Culture and transfer of human blastocysts increases implantation rates and reduces the need for multiple embryo transfers. , 1998, Fertility and sterility.

[10]  Shau-Ping Lin,et al.  Development of hatching blastocysts from immature human oocytes following in-vitro maturation and fertilization using a co-culture system. , 1998, Human reproduction.

[11]  L A Scott,et al.  The successful use of pronuclear embryo transfers the day following oocyte retrieval. , 1998, Human reproduction.

[12]  A. Trounson,et al.  Blastocyst transfer after enzymatic treatment of the zona pellucida: improving in-vitro fertilization and understanding implantation. , 1998, Human reproduction.

[13]  A. Templeton,et al.  Reducing the risk of multiple births by transfer of two embryos after in vitro fertilization. , 1998, The New England journal of medicine.

[14]  M. Tsirigotis Blastocyst stage transfer: pitfalls and benefits. Too soon to abandon current practice? , 1998, Human reproduction.

[15]  R. Winston,et al.  Glutamine improves human preimplantation development in vitro. , 1998, Fertility and sterility.

[16]  D. Gardner,et al.  Culture of viable human blastocysts in defined sequential serum-free media. , 1998, Human reproduction.

[17]  D. Gardner,et al.  A prospective randomized trial of blastocyst culture and transfer in in-vitro fertilization. , 1998, Human reproduction.

[18]  E. C. Wood,et al.  Evolution of a culture protocol for successful blastocyst development and pregnancy. , 1998, Human reproduction.

[19]  Y Verlinsky,et al.  Mosaicism in the inner cell mass of human blastocysts. , 1998, Human reproduction.

[20]  A. Trounson,et al.  Factors affecting the success of human blastocyst development and pregnancy following in vitro fertilization and embryo transfer. , 1998, Fertility and sterility.

[21]  D H Barlow,et al.  Heparin-binding epidermal growth factor significantly improves human blastocyst development and hatching in serum-free medium. , 1998, Human reproduction.

[22]  C. Jansen,et al.  The predictive value of day 3 embryo morphology regarding blastocyst formation, pregnancy and implantation rate after day 5 transfer following in-vitro fertilization or intracytoplasmic sperm injection. , 1998, Human reproduction.

[23]  H. Welch,et al.  Probabilities for singleton and multiple pregnancies after in vitro fertilization. , 1998, Fertility and sterility.

[24]  T. Pool,et al.  Improved clinical outcomes for in vitro fertilization with delay of embryo transfer from 48 to 72 hours after oocyte retrieval: use of glucose- and phosphate-free media. , 1998, Fertility and sterility.

[25]  N. Desai,et al.  Co-cultured human embryos may be subjected to widely different microenvironments: pattern of growth factor/cytokine release by Vero cells during the co-culture interval. , 1998, Human reproduction.

[26]  B. Dale,et al.  Time to switch from co-culture to sequential defined media for transfer at the blastocyst stage. , 1998, Human reproduction.

[27]  A. Veiga,et al.  Laser blastocyst biopsy for preimplantation diagnosis in the human , 1997, Zygote.

[28]  G. Zeilmaker,et al.  Blastocyst transfer in day-5 embryo transfer depends primarily on the number of oocytes retrieved and not on age. , 1998, Fertility and sterility.

[29]  R. Winston,et al.  Routine addition of human insulin-like growth factor-I ligand could benefit clinical in-vitro fertilization culture. , 1998, Human reproduction.

[30]  D. Gardner,et al.  No longer neglected: the human blastocyst. , 1998, Human reproduction.

[31]  C. Wun,et al.  Blastocyst formation is a good indicator for attainment of assisted reproduction. , 1997, Chinese journal of physiology.

[32]  D. Gardner,et al.  Development of serum-free media for the culture and transfer of human blastocysts. , 1998, Human reproduction.