CD44 is involved in CXCL-12 induced acute myeloid leukemia HL-60 cell polarity.

CXCL-12 and its receptor CXCR4 participate in breast cancer and melanoma cell metastasis to bone and lymphoid nodes. CD44, as a receptor for hyaluronic acid, is involved in lymphocyte recirculation, homing, adhesion and migration. But the role of CD44 in CXCL-12 induced leukemia cell migration still remains unclear. The present study showed that CXCL-12 stimulation induced the rapid internalization of CXCR4 and facilitated the formation of lamellipodia and uropod in acute leukemia cell line HL-60. CXCL-12 also induced CD44 translocation into the uropod, while CD44 remained evenly distributed on the untreated cell membranes. Results suggest that CD44 participates in CXCL-12 induced cell polarization and subsequent cell migration.

[1]  B. Wood,et al.  Myeloid malignancies: myelodysplastic syndromes, myeloproliferative disorders, and acute myeloid leukemia. , 2007, Clinics in laboratory medicine.

[2]  K. Bogunia-Kubik,et al.  [The role of the SDF-1-CXCR4 axis in hematopoiesis and the mobilization of hematopoietic stem cells to peripheral blood]. , 2007, Postepy higieny i medycyny doswiadczalnej.

[3]  J. Burger,et al.  The CXCR4 chemokine receptor in acute and chronic leukaemia: a marrow homing receptor and potential therapeutic target , 2007, British journal of haematology.

[4]  R. Savani,et al.  Hyaluronan-mediated angiogenesis in vascular disease: uncovering RHAMM and CD44 receptor signaling pathways. , 2007, Matrix biology : journal of the International Society for Matrix Biology.

[5]  R. Weinberg,et al.  Chemokine networks and breast cancer metastasis. , 2006, Breast disease.

[6]  Jianhua Wang,et al.  The pivotal role of CXCL12 (SDF-1)/CXCR4 axis in bone metastasis , 2007, Cancer and Metastasis Reviews.

[7]  T. Kipps,et al.  CXCR4: a key receptor in the crosstalk between tumor cells and their microenvironment. , 2006, Blood.

[8]  K. Brown,et al.  Regulation of hyaluronan binding by F-actin and colocalization of CD44 and phosphorylated ezrin/radixin/moesin (ERM) proteins in myeloid cells. , 2005, Experimental cell research.

[9]  T. Hara,et al.  Roles of p-ERM and Rho–ROCK signaling in lymphocyte polarity and uropod formation , 2004, The Journal of cell biology.

[10]  J. Delaunay,et al.  The Effect of Anti-CD44 Monoclonal Antibodies on Differentiation and Proliferation of Human Acute Myeloid Leukemia Cells , 2004, Leukemia & lymphoma.

[11]  R. Alon,et al.  CD44 and hyaluronic acid cooperate with SDF-1 in the trafficking of human CD34+ stem/progenitor cells to bone marrow. , 2004, Blood.

[12]  Arnon Nagler,et al.  CXCR4 regulates migration and development of human acute myelogenous leukemia stem cells in transplanted NOD/SCID mice. , 2004, Cancer research.

[13]  L. Bourguignon,et al.  Hyaluronan-mediated CD44 Interaction with RhoGEF and Rho Kinase Promotes Grb2-associated Binder-1 Phosphorylation and Phosphatidylinositol 3-Kinase Signaling Leading to Cytokine (Macrophage-Colony Stimulating Factor) Production and Breast Tumor Progression* , 2003, Journal of Biological Chemistry.

[14]  C. Jasmin,et al.  Effects of anti-CD44 monoclonal antibodies on differentiation and apoptosis of human myeloid leukemia cell lines. , 2002, Blood.

[15]  T. Springer,et al.  The Chemokine SDF-1 Is a Chemoattractant for Human CD34+ Hematopoietic Progenitor Cells and Provides a New Mechanism to Explain the Mobilization of CD34+ Progenitors to Peripheral Blood , 1997, The Journal of experimental medicine.

[16]  P. Herrlich,et al.  CD44 splice variants: expression during lymphocyte activation and tumor progression. , 1993, Behring Institute Mitteilungen.

[17]  M. Telen,et al.  CD44--a molecule involved in leukocyte adherence and T-cell activation. , 1989, Immunology today.