Osteogenic capacity of alkali-free bioactive glasses. In vitro studies.

The high alkali content bioactive glasses commonly used to regenerate bone in dental, orthopedic, and maxillofacial surgeries induce some cytotoxicity in surrounding tissues. The present study aims the ability of some alkali-free bioactive glasses compositions, recently developed by our research group, to stimulate human mesenchymal stem cells (hMSCs) differentiation into osteoblasts in comparison to 45S5 Bioglass® . The obtained results demonstrated that alkali-free bioactive glasses possess higher stimulating towards differentiation of hMSCs in comparison to the control 45S5 Bioglass® . The von Kossa assay demonstrated that all bioactive glasses studied were able to induce the appearance of calcium deposits even when the cells were cultured in DMEM, proving that these biomaterials per se induce hMSCs cell differentiation. It was also observed that in both cell culture medium used (DMEM, and osteogenesis differentiation medium) alkali-free bioactive glasses clearly induced the appearance of more calcium deposits than the 45S5 Bioglass® , indicating their greater ability to induce cell differentiation. In summary, these results indicate that alkali-free bioactive glasses are a safe and valid alternative to 45S5 Bioglass® . © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2360-2365, 2017.

[1]  Jincheng Du,et al.  Understanding the composition-structure-bioactivity relationships in diopside (CaO·MgO·2SiO₂)-tricalcium phosphate (3CaO·P₂O₅) glass system. , 2015, Acta biomaterialia.

[2]  Ian M. Reaney,et al.  The osteogenic response of mesenchymal stromal cells to strontium‐substituted bioactive glasses , 2015, Journal of tissue engineering and regenerative medicine.

[3]  J. Ferreira,et al.  Structure, biodegradation behavior and cytotoxicity of alkali-containing alkaline-earth phosphosilicate glasses. , 2014, Materials science & engineering. C, Materials for biological applications.

[4]  Antonio Tilocca,et al.  Role of glass structure in defining the chemical dissolution behavior, bioactivity and antioxidant properties of zinc and strontium co-doped alkali-free phosphosilicate glasses. , 2014, Acta biomaterialia.

[5]  M. Pascual,et al.  Thermo-mechanical behaviour of alkali free bioactive glass-ceramics co-doped with strontium and zinc , 2013 .

[6]  José M.F. Ferreira,et al.  Structural role of zinc in biodegradation of alkali-free bioactive glasses. , 2013, Journal of materials chemistry. B.

[7]  L. Bonewald,et al.  Evaluation of bone regeneration, angiogenesis, and hydroxyapatite conversion in critical-sized rat calvarial defects implanted with bioactive glass scaffolds. , 2012, Journal of biomedical materials research. Part A.

[8]  A. Boccaccini,et al.  Magnesium‐Containing Bioactive Glasses for Biomedical Applications , 2012 .

[9]  D. Brauer,et al.  Fluoride-containing bioactive glass-ceramics , 2012 .

[10]  D. Liang,et al.  Zinc Upregulates the Expression of Osteoprotegerin in Mouse Osteoblasts MC3T3-E1 Through PKC/MAPK Pathways , 2012, Biological Trace Element Research.

[11]  Rozalia Dimitriou,et al.  Bone regeneration: current concepts and future directions , 2011, BMC medicine.

[12]  M. Nagata,et al.  Role of zinc in cellular zinc trafficking and mineralization in a murine osteoblast-like cell line. , 2011, The Journal of nutritional biochemistry.

[13]  Sreeram V Ramagopalan,et al.  Risk of venous thromboembolism in people admitted to hospital with selected immune-mediated diseases: record-linkage study , 2011, BMC medicine.

[14]  Delbert E. Day,et al.  Glass and Medicine , 2010 .

[15]  M Vogel,et al.  In vivo comparison of bioactive glass particles in rabbits. , 2001, Biomaterials.

[16]  P. Hatton,et al.  Influence of sodium oxide content on bioactive glass properties , 1999, Journal of materials science. Materials in medicine.

[17]  Larry L. Hench,et al.  Bioceramics: From Concept to Clinic , 1991 .

[18]  S. Mcgee-Russell.  HISTOCHEMICAL METHODS FOR CALCIUM , 1958, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[19]  Julian R Jones,et al.  Review of bioactive glass: from Hench to hybrids. , 2013, Acta biomaterialia.

[20]  José M.F. Ferreira,et al.  Alkali-free bioactive glasses for bone tissue engineering: a preliminary investigation. , 2012, Acta biomaterialia.

[21]  D. Prockop,et al.  Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. , 2006, Cytotherapy.

[22]  Patries M Herst,et al.  Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. , 2005, Biotechnology annual review.