Development and growth of axonal sprouts from noradrenaline and 5-hydroxytryptamine neurones in the rat spinal cord.

Summary Compressions of, or transplantations to, the rat spinal cord were studied with the Falck-Hillarp fluorescence method for monoamines. Newly formed axon sprouts were observed from the severed preterminal axons of both noradrenaline and 5-hydroxytryptamine (5-HT) neurones in the white matter. The first traces of delicate newly formed fibres were observed after 2–3 days, and they rapidly increased in number during the first two weeks. The sprouting fibres were seen to grow into the necrosis. In the transplanted animals the catecholamine-containing fibre sprouts grew across a necrotic zone and reached the transplanted tissue, where they arranged themselves in a pattern resembling that of the normal adrenergic innervation of the transplanted tissue. Although the 5-HT-containing neurones showed abundant sprouting into the necrosis around the transplanted tissue, such fibres were never seen to grow into or on the surface of the transplant.

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