Cloning and Expression Analysis of Phosphoenolpyruvate Carboxylase Gene pepc from Cassava(Manihot esculenta Crantz)

The full-length cDNAs of phosphoenolpyruvate carboxylase gene pepc were cloned from cassava(Manihot esculenta) cultivar Arg7 and wild species(M.esculenta subsp.flabellifolia) W14 by using Nest-PCR method,respectively,with corresponding GenBank accession number of JN387053 and JN387052.Both of two cDNA sequences were 2945 bp in length including a ORF of 2895 bp,predicting to encode a protein with 964 amino acids and containing typical conserved sequences/domains of pepc gene.Cassava PEPC had high homologous with that in Ricinus communis and Jatropha curcas.The pepc expression was the highest in leaves of W14 and Arg7,followed fibrous roots and tuberous roots,and the lowest expression was in stems.The one-day dynamic expression analysis showed that the pepc expression in Arg7 leaves was higher than in W14 before 16∶00,while it was higher in W14 than Arg7 after 16∶00.Therefore,it suggested that the regulatory regions of pepc gene in two cassava species were different.

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