Robust cellulosic ethanol production from SPORL-pretreated lodgepole pine using an adapted strain Saccharomyces cerevisiae without detoxification.

This study reports an ethanol yield of 270L/ton wood from lodgepole pine pretreated with sulfite pretreatment to overcome recalcitrance of lignocellulose (SPORL) using an adapted strain, Saccharomyces cerevisiae Y5, without detoxification. The enzymatic hydrolysate produced from pretreated cellulosic solids substrate was combined with pretreatment hydrolysate before fermentation. Detoxification of the pretreatment hydrolysate using overliming or XAD-4 resin before being combined with enzymatic hydrolysate improved ethanol productivity in the first 4h of fermentation and overall fermentation efficiency. However, detoxification did not improve final ethanol yield because of sugar losses. The Y5 strain showed excellent ethanol productivities of 2.0 and 0.8g/L/h averaged over a period of 4 and 24h, respectively, in the undetoxified run. The furan metabolization rates of the Y5 strain were significantly higher for the undetoxified run than those for the detoxidfied runs, suggesting it can tolerate even higher furan concentrations than those studied. Preliminary mass and energy balances were conducted. SPORL produced an excellent monomeric sugar recovery value of about 85% theoretical and a net energy output of 4.05GJ/ton wood with an ethanol energy production efficiency of 178% before distillation.

[1]  Wenjie Zhu,et al.  On energy consumption for size-reduction and yields from subsequent enzymatic saccharification of pretreated lodgepole pine. , 2010, Bioresource technology.

[2]  J. Y. Zhu,et al.  Sulfite pretreatment to overcome recalcitrance of lignocellulose (SPORL) for robust enzymatic saccharification of hardwoods , 2009, Biotechnology progress.

[3]  Bruce E. Dale,et al.  Cellulosic ethanol production from AFEX-treated corn stover using Saccharomyces cerevisiae 424A(LNH-ST) , 2009, Proceedings of the National Academy of Sciences.

[4]  J. Y. Zhu,et al.  Ethanol production from SPORL-pretreated lodgepole pine: preliminary evaluation of mass balance and process energy efficiency , 2010, Applied Microbiology and Biotechnology.

[5]  C. Cardona,et al.  Production of bioethanol from sugarcane bagasse: Status and perspectives. , 2010, Bioresource technology.

[6]  Michael R. Ladisch,et al.  Removal of Fermentation Inhibitors Formed during Pretreatment of Biomass by Polymeric Adsorbents , 2002 .

[7]  J. Saddler,et al.  Acid‐catalyzed steam pretreatment of lodgepole pine and subsequent enzymatic hydrolysis and fermentation to ethanol , 2007, Biotechnology and bioengineering.

[8]  Rishi Gupta,et al.  Separate hydrolysis and fermentation (SHF) of Prosopis juliflora, a woody substrate, for the production of cellulosic ethanol by Saccharomyces cerevisiae and Pichia stipitis-NCIM 3498. , 2009, Bioresource technology.

[9]  J. Baeza,et al.  Bioethanol production from bio- organosolv pulps of Pinus radiata and Acacia dealbata , 2007 .

[10]  Mark Holtzapple,et al.  Comparative sugar recovery and fermentation data following pretreatment of poplar wood by leading technologies , 2009, Biotechnology progress.

[11]  J. Y. Zhu,et al.  Sulfite pretreatment (SPORL) for robust enzymatic saccharification of spruce and red pine. , 2009, Bioresource technology.

[12]  D. Evtuguin,et al.  Second-generation bioethanol from eucalypt sulphite spent liquor. , 2010, Bioresource technology.

[13]  T. Jeffries,et al.  Second generation bioethanol production from Saccharum spontaneum L. ssp. aegyptiacum (Willd.) Hack. , 2010, Bioresource technology.

[14]  J. Y. Zhu,et al.  Woody biomass pretreatment for cellulosic ethanol production: Technology and energy consumption evaluation. , 2010, Bioresource technology.

[15]  Qiang Yang,et al.  Comparative study of SPORL and dilute-acid pretreatments of spruce for cellulosic ethanol production. , 2010, Bioresource technology.

[16]  C. Wyman,et al.  Pretreatment: the key to unlocking low‐cost cellulosic ethanol , 2008 .

[17]  G. Guo,et al.  Enhanced ethanol production by fermentation of rice straw hydrolysate without detoxification using a newly adapted strain of Pichia stipitis. , 2009, Bioresource technology.

[18]  M. Galbe,et al.  Steam pretreatment of H(2)SO(4)-impregnated Salix for the production of bioethanol. , 2008, Bioresource technology.

[19]  Ke-Ke Cheng,et al.  Improved 2,3-butanediol production from corncob acid hydrolysate by fed-batch fermentation using Klebsiella oxytoca , 2010 .

[20]  R. Ocampo-Pérez,et al.  Adsorption of Fluoride from Water Solution on Bone Char , 2007 .

[21]  Ronald S. Zalesny,et al.  Pretreatment of woody biomass for biofuel production: energy efficiency, technologies, and recalcitrance , 2010, Applied Microbiology and Biotechnology.