Nitrogen removal from wastewater using simultaneous nitrate reduction and anaerobic ammonium oxidation in single reactor.

The effects of C/N ratio and total organic carbon (TOC) loading on nitrogen removal through simultaneous nitrate reduction and anaerobic ammonium oxidation in a single reactor were examined. Granular sludge taken from a methane fermentation reactor was placed in an upflow reactor and supplied with synthetic wastewater containing nitrate at a C/N ratio of 1 to grow heterotrophic denitrifying bacteria. When nitrogen removal ratio reached 30%, anammox sludge attached to nonwoven-carrier was added into the same reactor and then ammonia was added to the synthetic wastewater. Nitrogen removal ratio was markedly increased to 80-94%. In this system, nitrogen removal ratio was affected by C/N ratio and TOC loading, not by the amount of granular sludge. A stable isotopic analysis using 15N-labeled nitrate showed that N2 gas was formed by anammox reaction.

[1]  E. Stackebrandt,et al.  Nucleic acid techniques in bacterial systematics , 1991 .

[2]  K. Furukawa,et al.  Single-Stage Nitrogen Removal Using Anammox and Partial Nitritation (SNAP) for Treatment of Synthetic Landfill Leachate , 2005 .

[3]  K. Schleifer,et al.  The domain-specific probe EUB338 is insufficient for the detection of all Bacteria: development and evaluation of a more comprehensive probe set. , 1999, Systematic and applied microbiology.

[4]  Hiroki Nakamura,et al.  High rate and compact single sludge pre-denitrification process for retrofit , 1994 .

[5]  W. Zumft,et al.  The Denitrifying Prokaryotes , 1992 .

[6]  David A. Stahl,et al.  Development and application of nucleic acid probes , 1991 .

[7]  K. Schleifer,et al.  Molecular evidence for genus level diversity of bacteria capable of catalyzing anaerobic ammonium oxidation. , 2000, Systematic and applied microbiology.

[8]  W. Verstraete,et al.  Characterization of an Autotrophic Nitrogen-Removing Biofilm from a Highly Loaded Lab-Scale Rotating Biological Contactor , 2003, Applied and Environmental Microbiology.

[9]  S. Tsuneda,et al.  Growth characteristic of anaerobic ammonium-oxidizing bacteria in an anaerobic biological filtrated reactor , 2006, Applied Microbiology and Biotechnology.

[10]  Y. Kitagawa,et al.  Microbial analyses by fluorescence in situ hybridization of well-settled granular sludge in brewery wastewater treatment plants. , 2002, Journal of bioscience and bioengineering.

[11]  J. G. Kuenen,et al.  Anaerobic ammonium oxidation discovered in a denitrifying fluidized bed reactor , 1995 .

[12]  J. G. Kuenen,et al.  Combined nitrification-denitrification processes , 1994 .

[13]  M. Jetten,et al.  CANON and Anammox in a gas-lift reactor. , 2003, FEMS microbiology letters.

[14]  M. V. van Loosdrecht,et al.  The SHARON-Anammox process for treatment of ammonium rich wastewater. , 2001, Water science and technology : a journal of the International Association on Water Pollution Research.