Base pairing of RNA I with its complementary sequence in the primer precursor inhibits ColE1 replication

Four single base pair mutations in the region that codes for RNA I create new incompatibility groups while preserving the mechanism of control of initiation of DNA replication in a ColE1-type plasmid. Sequence analysis of these base substitutions points to the primary importance of the central loop of the cloverleaf structure of RNA I in this control mechanism.

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