论文信息 - Directed Evolution of CRISPR-Cas9 Base Editors.

Directed Evolution of CRISPR-Cas9 Base Editors.

A recent publication by Thuronyi et al. described a directed evolution system called phage-assisted continuous evolution (PACE) that was used to generate improved variants of CRISPR-Cas9 base editors. These evolved base editors overcome some of the inherent limitations of the technology such as sequence context preferences, restricted editing windows, and large construct sizes.

Pablo Perez-Pinera | Jackson Winter | P. Pérez‐Piñera | Jackson Winter | P. Perez-Pinera

[1] A. D’Andrea,et al. Repair Pathway Choices and Consequences at the Double-Strand Break. , 2016, Trends in cell biology.

[2] Gregory McAllister,et al. p53 inhibits CRISPR–Cas9 engineering in human pluripotent stem cells , 2018, Nature Medicine.

[3] Reuben S Harris,et al. Comparison of the differential context-dependence of DNA deamination by APOBEC enzymes: correlation with mutation spectra in vivo. , 2004, Journal of molecular biology.

[4] David R. Liu,et al. Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage , 2016, Nature.

[5] David R. Liu,et al. Continuous evolution of base editors with expanded target compatibility and improved activity , 2019, Nature Biotechnology.

[6] Dana Carroll,et al. Nucleosomes inhibit target cleavage by CRISPR-Cas9 in vivo , 2018, Proceedings of the National Academy of Sciences.

[7] Jussi Taipale,et al. CRISPR–Cas9 genome editing induces a p53-mediated DNA damage response , 2018, Nature Medicine.

[8] A. Bradley,et al. Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements , 2018, Nature Biotechnology.

[9] David R. Liu,et al. A System for the Continuous Directed Evolution of Biomolecules , 2011, Nature.

[10] Nicole M. Gaudelli,et al. Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage , 2017, Nature.