Sporoderm in Populus and Salix

Abstract Four phenomena were observed in a study of Populus tremula and P. tremula f. gigas microspores from before microspore mitosis through mature pollen which may have general significance in the ontogeny of pollen grains: 1) The exine and orbicules (Ubisch bodies) were covered by membranes. 2) The exine and the tapetal surfaces where orbicules form were covered by a polysaccharide (PAS positive) coat until after microspore mitosis; subsequently the tapetum became plasmodial. 3) Material having the staining characteristics of the nexine 2 (endexine in the sense of Faegri) accumulated on membranes in microspores in the space between the exine and the plasma membrane. That material was almost completely gone from the wall in mature pollen. The membranes on which material had accumulated migrated through the exine. Following passage through the exine these membranes were seen as empty fusiform vesicles in micrographs of anthers prepared by commonly used methods. 4) At about microspore mitosis when the cel...

[1]  Christian de Duve,et al.  The Lysosome Concept , 2008 .

[2]  J. F. López-Sáez,et al.  Fine structure of the plasmodesm , 1966, Protoplasma.

[3]  Lazaro J. Mandel,et al.  Cell calcium and the control of membrane transport. , 1987, Society of General Physiologists series.

[4]  J. Rowley.  Fibrils, microtubules and lamellae in pollen grains , 1967 .

[5]  P. Echlin,et al.  The development of the pollen grain wall in Ipomoea purpurea (L.) Roth , 1967 .

[6]  R. Angold.  The ontogeny and fine structure of the pollen grain of Endymion non-scriptus , 1967 .

[7]  D. Southworth,et al.  Deposition of Sporopollenin on Lamellae of Unit Membrane Dimensions , 1967, Nature.

[8]  A. R. Freeman,et al.  Plasma Membrane: Substructural Changes Correlated with Electrical Resistance and Pinocytosis , 1967, Science.

[9]  U. Banerjee.  Ultrastructure of the Tapetal Membranes in Grasses , 1967 .

[10]  H. Ragetli,et al.  LATENT ACID PHOSPHATASE IN CHLOROPLASTS , 1966 .

[11]  C. C. Bowen,et al.  SPORE DEVELOPMENT IN THE LIVERWORT RICCARDIA PINGUIS , 1966 .

[12]  D. A. Larson,et al.  FINE STRUCTURAL STUDIES OF ZEA MAYS POLLEN I: CELL MEMBRANES AND EXINE ONTOGENY , 1966 .

[13]  E. Eigenbrodt,et al.  HEPATIC INTRACELLULAR OSMIOPHILIC DROPLETS: Effect of Lipid Solvents during Tissue Preparation , 1966 .

[14]  M. Farquhar,et al.  LYSOSOME FUNCTION IN THE REGULATION OF THE SECRETORY PROCESS IN CELLS OF THE ANTERIOR PITUITARY GLAND , 1966, The Journal of cell biology.

[15]  A. Cohen,et al.  ON THE PRIMARY SITE OF NUCLEAR RNA SYNTHESIS , 1966, The Journal of cell biology.

[16]  E. Korn.  Structure of Biological Membranes , 1966, Science.

[17]  M. Gail,et al.  PHYSICOCHEMICAL DIFFERENCES BETWEEN FRAGMENTS OF PLASMA MEMBRANE AND ENDOPLASMIC RETICULUM , 1966, The Journal of cell biology.

[18]  R. M. Hicks.  THE FUNCTION OF THE GOLGI COMPLEX IN TRANSITIONAL EPITHELIUM , 1966, The Journal of cell biology.

[19]  J. Skvarla,et al.  POLLEN WALL ULTRASTRUCTURE AND ITS BEARING ON THE SYSTEMATIC POSITION OF BLENNOSPERMA AND CROCIDIUM (COMPOSITAE) , 1966 .

[20]  E. Korn,et al.  I. Loss of lipids during preparation of amoebae for electron microscopy. , 1966, Biochimica et biophysica acta.

[21]  J. Heslop-Harrison.  Cytoplasmic Connexions between Angiosperm Meiocytes , 1966 .

[22]  D. A. Larson.  On the Significance of the Detailed Structure of Passiflora caerulea Exines , 1966, Botanical Gazette.

[23]  J. Pettitt.  A new interpretation of the structure of the megaspore membrane in some gymnospermous ovules , 1966 .

[24]  J. Skvarla,et al.  Systematic Implications from Electron Microscopic Studies of Compositae Pollen-A Review , 1966 .

[25]  B. Gullvåg.  The Fine Structure of Some Gymnosperm Pollen Walls , 1966 .

[26]  D. Southworth.  Ultrastructure of Gerbera Jamesonii Pollen , 1966 .

[27]  J. Rowley,et al.  Single-stage carbon replicas of microspores. , 1966, Stain technology.

[28]  G. Shaw,et al.  Chemical studies on the constitution of some pollen and spore membranes. , 1966, Journal of the Chemical Society. Perkin transactions 1.

[29]  G. Rossi,et al.  Osservazioni Sulla Morfologia Dei Lomasomi , 1964 .

[30]  J. Rowley,et al.  Identification of Modern and Fossil Maize Pollen , 1964 .

[31]  E. Reynolds.  THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY , 1963, The Journal of cell biology.

[32]  H. S. Bennett.  MORPHOLOGICAL ASPECTS OF EXTRACELLULAR POLYSACCHARIDES , 1963 .

[33]  J. Heslop-Harrison.  An Ultrastructural Study of Pollen Wall Ontogeny in Silene Pendula , 1963 .

[34]  J. Heslop-Harrison.  Origin of Exine , 1962, Nature.

[35]  J. Praglowski.  Notes on the Pollen Morphology of Swedish Trees and Shrubs , 1962 .

[36]  J. Rowley.  Nonhomogeneous Sporopollenin in Microspores of Poa Annua L , 1962 .

[37]  D. A. Larson,et al.  Pollen Wall Development in Parkinsonia Aculeata , 1962 .

[38]  M. Takeoka,et al.  Electron-Microscope Investigations of the Fine Details of the Pollen Grain Surface in Japanese Gymnosperms , 1962 .

[39]  M. Karnovsky.  SIMPLE METHODS FOR "STAINING WITH LEAD" AT HIGH pH IN ELECTRON MICROSCOPY , 1961, The Journal of biophysical and biochemical cytology.

[40]  John H. Luft,et al.  IMPROVEMENTS IN EPOXY RESIN EMBEDDING METHODS , 1961, The Journal of biophysical and biochemical cytology.

[41]  B. Raj.  Pollen morphological studies in the Acanthaceae , 1961 .

[42]  P. Brandt,et al.  AN ELECTRON MICROSCOPIC STUDY OF PINOCYTOSIS IN AMEBA , 1960, The Journal of biophysical and biochemical cytology.

[43]  G. Palade,et al.  Segregation of Ferritin in Glomerular Protein Absorption Droplets , 1960, The Journal of biophysical and biochemical cytology.

[44]  E. Stix.  Pollenmorphologische Untersuchungen an Compositen , 1960 .

[45]  P. Sitte.  Polarisationsmikroskopische Untersuchungen an Sporoderme , 1959 .

[46]  J. Ueno.  Some palynological observations of Taxaceae, Cupressaceae and Araucariaceae , 1959 .

[47]  J. D. Robertson,et al.  The ultrastructure of cell membranes and their derivatives. , 1959, Biochemical Society symposium.

[48]  H. Ehrlich.  Electron microscope studies of Saintpaulia ionantha Wendl. pollen walls. , 1958, Experimental cell research.

[49]  D. Hanahan,et al.  A column chromatographic separation of classes of phospholipides. , 1957, The Journal of biological chemistry.

[50]  D. von Wettstein,et al.  Chlorophyll-letale und der submikroskopische Formwechsel der Plastiden , 1957 .

[51]  K. Fægri.  On the Peri-Glacial Flora of Jæren. With Notes on the Morphology of the Salix Pollen Grain , 1953 .

[52]  A. Dahl,et al.  Electron microscopy of ultrathin frozen sections of pollen grains. , 1952, Science.

[53]  Hotchkiss Rd,et al.  A microchemical reaction resulting in the staining of polysaccharide structures in fixed tissue preparations. , 1948 .