Development of a pericardial acellular matrix biomaterial: biochemical and mechanical effects of cell extraction.

There is evidence to suggest that the cellular components of homografts and bioprosthetic xenografts may contribute to calcification or immunogenic reactions. A four-step detergent and enzymatic extraction process has been developed to remove cellular components from bovine pericardial tissue. The process results in an acellular matrix material consisting primarily of elastin, insoluble collagen, and tightly bound glycosaminoglycans. Light and electron microscopy confirmed that nearly all cellular constituents are removed without ultrastructural evidence of damage to fibrous components. Collagen denaturation temperatures remained unaltered. Biochemical analysis confirmed the retention of collagen and elastin and some differential extraction of glycosaminoglycans. Low strain rate fracture testing and high strain rate viscoelastic characterization showed that, with the exception of slightly increased stress relaxation, the mechanical properties of the fresh tissue were preserved in the pericardial acellular matrix. Crosslinking of the material in glutaraldehyde or poly(glycidyl ether) produced mechanical changes consistent with the same treatments of fresh tissue. The pericardial acellular matrix is a promising approach to the production of biomaterials for heart valve or cardiovascular patching applications.

[1]  R. Iozzo,et al.  Bovine pericardial proteoglycan: biochemical, immunochemical and ultrastructural studies. , 1989, Matrix.

[2]  L. Sandberg,et al.  A new method for purification of mature elastin. , 1975, Analytical biochemistry.

[3]  B. Strates,et al.  Chemically modified collagen: a natural biomaterial for tissue replacement. , 1987, Journal of biomedical materials research.

[4]  F J Schoen,et al.  Bioprosthetic heart valve failure: pathology and pathogenesis. , 1984, Cardiology clinics.

[5]  A. Schwell,et al.  Immunogenicity of glutaraldehyde-tanned bovine pericardium. , 1990, The Journal of thoracic and cardiovascular surgery.

[6]  R. Levy,et al.  The role of glutaraldehyde-induced cross-links in calcification of bovine pericardium used in cardiac valve bioprostheses. , 1987, The American journal of pathology.

[7]  E. A. Trowbridge,et al.  The tensile strength of natural and chemically modified bovine pericardium. , 1988 .

[8]  K. Kivirikko,et al.  Modifications of a specific assay for hydroxyproline in urine. , 1967, Analytical biochemistry.

[9]  L Gonzalez-Lavin,et al.  Causes of failure and pathologic findings in surgically removed Ionescu-Shiley standard bovine pericardial heart valve bioprostheses: emphasis on progressive structural deterioration. , 1987, Circulation.

[10]  G. L’italien,et al.  Glutaraldehyde release from vascular prostheses of biologic origin. , 1988, Surgery.

[11]  B. Starcher,et al.  Purification and comparison of elastins from different animal species. , 1976, Analytical biochemistry.

[12]  D. Simionescu,et al.  Partial characterization of a low molecular weight proteoglycan isolated from bovine parietal pericardium. , 1988, Biochemical and biophysical research communications.

[13]  J M Lee,et al.  Effect of alternative crosslinking methods on the low strain rate viscoelastic properties of bovine pericardial bioprosthetic material. , 1990, Journal of biomedical materials research.

[14]  F J Schoen,et al.  Calcification of subcutaneously implanted type I collagen sponges. Effects of formaldehyde and glutaraldehyde pretreatments. , 1986, The American journal of pathology.

[15]  D A Cooley,et al.  Ionescu-Shiley bovine pericardial bioprostheses. Histologic and ultrastructural studies. , 1992, The American journal of pathology.

[16]  F. Schoen,et al.  Phosphatase enzyme activity is retained in glutaraldehyde treated bioprosthetic heart valves. , 1988, ASAIO transactions.

[17]  U. Losert,et al.  Biocompatibility of aldehyde-fixed bovine pericardium. An in vitro and in vivo approach toward improvement of bioprosthetic heart valves. , 1991, The Journal of thoracic and cardiovascular surgery.

[18]  J M Lee,et al.  The bovine pericardial xenograft: II. Effect of tethering or pressurization during fixation on the tensile viscoelastic properties of bovine pericardium. , 1989, Journal of biomedical materials research.

[19]  G Thiene,et al.  Ultrastructural substrates of dystrophic calcification in porcine bioprosthetic valve failure. , 1985, The American journal of pathology.

[20]  M. Lomonosov,et al.  Biochemical and physico-chemical aspects of biomaterials calcification. , 1991, Biomaterials.

[21]  F. Keeley,et al.  Response of aortic elastin synthesis and accumulation to developing hypertension and the inhibitory effect of colchicine on this response. , 1991, Laboratory investigation; a journal of technical methods and pathology.

[22]  J. Castillo-olivares,et al.  Study of the calcification of bovine pericardium: analysis of the implication of lipids and proteoglycans. , 1991, Biomaterials.

[23]  Lee Jm,et al.  The bovine pericardial xenograft: I. Effect of fixation in aldehydes without constraint on the tensile viscoelastic properties of bovine pericardium , 1989 .

[24]  J. Castillo-olivares,et al.  Calcification of soft tissue employed in the construction of heart valve prostheses: study of different chemical treatments. , 1991, Biomaterials.

[25]  D. Eyre,et al.  Collagen: molecular diversity in the body's protein scaffold , 1980, Science.

[26]  T. Wight,et al.  Cell biology of arterial proteoglycans. , 1989, Arteriosclerosis.

[27]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[28]  R L Jackson,et al.  Glycosaminoglycans: molecular properties, protein interactions, and role in physiological processes. , 1991, Physiological reviews.