Integrin  V is necessary for gastrulation movements that regulate vertebrate body asymmetry

laterality phenotype of  V morphants. When tracked in real-time, individual DFCs of both morphants showed defects in DFC migration, preventing them from organizing into a KV of normal shape and size. Thus, we propose that  V  1b mediates cellular interactions that are necessary for DFC clustering and movements necessary for Kupffer’s vesicle formation, uncovering an early contribution of integrins to the regulation of vertebrate laterality.

[1]  S. Holley,et al.  Control of extracellular matrix assembly along tissue boundaries via Integrin and Eph/Ephrin signaling , 2009, Development.

[2]  M. Mullins,et al.  Early zebrafish development: it's in the maternal genes. , 2009, Current opinion in genetics & development.

[3]  M. Labouesse.  Faculty Opinions recommendation of Galpha12/13 regulate epiboly by inhibiting E-cadherin activity and modulating the actin cytoskeleton. , 2009 .

[4]  Carl-Philipp Heisenberg,et al.  Origin and shaping of the laterality organ in zebrafish , 2008, Development.

[5]  A. Kuroiwa,et al.  Sdf1/Cxcr4 signaling controls the dorsal migration of endodermal cells during zebrafish gastrulation , 2008, Development.

[6]  J. Eisen,et al.  Controlling morpholino experiments: don't stop making antisense , 2008, Development.

[7]  D. Houston,et al.  Calcium fluxes in dorsal forerunner cells antagonize β-catenin and alter left-right patterning , 2007, Development.

[8]  H. Yost,et al.  Two T-box genes play independent and cooperative roles to regulate morphogenesis of ciliated Kupffer's vesicle in zebrafish. , 2007, Developmental biology.

[9]  Xiaojing Ye,et al.  The integrins , 2007, Genome Biology.

[10]  C. Carman,et al.  Structural basis of integrin regulation and signaling. , 2007, Annual review of immunology.

[11]  G. Jayson,et al.  Alpha-v Integrins as Therapeutic Targets in Oncology , 2007, Cancer investigation.

[12]  P. Skoglund,et al.  The presumptive floor plate (notoplate) induces behaviors associated with convergent extension in medial but not lateral neural plate cells of Xenopus. , 2006, Developmental biology.

[13]  J. C. Belmonte,et al.  Regulation of primary cilia formation and left-right patterning in zebrafish by a noncanonical Wnt signaling mediator, duboraya , 2006, Nature Genetics.

[14]  J. Huxley-Jones,et al.  Identification of multiple integrin β1 homologs in zebrafish (Danio rerio) , 2006, BMC Cell Biology.

[15]  G. Danuser,et al.  Morphodynamic profiling of protrusion phenotypes. , 2006, Biophysical journal.

[16]  R. Geisler,et al.  Integrinalpha5 and delta/notch signaling have complementary spatiotemporal requirements during zebrafish somitogenesis. , 2005, Developmental cell.

[17]  H. Yost,et al.  Kupffer's vesicle is a ciliated organ of asymmetry in the zebrafish embryo that initiates left-right development of the brain, heart and gut , 2005, Development.

[18]  D. Housman,et al.  Selective ablation of αv integrins in the central nervous system leads to cerebral hemorrhage, seizures, axonal degeneration and premature death , 2004, Development.

[19]  H. Yost,et al.  The T Box Transcription Factor No Tail in Ciliated Cells Controls Zebrafish Left-Right Asymmetry , 2004, Current Biology.

[20]  M. Rebagliati,et al.  The zebrafish nodal-related gene southpaw is required for visceral and diencephalic left-right asymmetry , 2003, Development.

[21]  D. Stainier,et al.  From endoderm formation to liver and pancreas development in zebrafish , 2003, Mechanisms of Development.

[22]  R. Hynes,et al.  Defective Associations between Blood Vessels and Brain Parenchyma Lead to Cerebral Hemorrhage in Mice Lacking αv Integrins , 2002, Molecular and Cellular Biology.

[23]  Richard O Hynes,et al.  Integrins Bidirectional, Allosteric Signaling Machines , 2002, Cell.

[24]  C. Tabin,et al.  Left–right development: Conserved function for embryonic nodal cilia , 2002, Nature.

[25]  I. Kirby,et al.  Adenovirus type 5 uptake by lung adenocarcinoma cells in culture correlates with Ad5 fibre binding is mediated by αvβ1 integrin and can be modulated by changes in β1 integrin function , 2001, Journal of Gene Medicine.

[26]  A. Aszódi,et al.  Functional consequences of integrin gene mutations in mice. , 2001, Circulation research.

[27]  J. Fawcett,et al.  Developmental Regulation of αv Integrins Produces Functional Changes in Astrocyte Behavior , 2001, Molecular and Cellular Neuroscience.

[28]  D. Stainier,et al.  casanova encodes a novel Sox-related protein necessary and sufficient for early endoderm formation in zebrafish. , 2001, Genes & development.

[29]  W. Driever,et al.  Pancreas development in zebrafish: early dispersed appearance of endocrine hormone expressing cells and their convergence to form the definitive islet. , 2001, Developmental biology.

[30]  S. Ekker,et al.  Effective targeted gene ‘knockdown’ in zebrafish , 2000, Nature Genetics.

[31]  H. Yost,et al.  Multiple pathways in the midline regulate concordant brain, heart and gut left-right asymmetry. , 2000, Development.

[32]  Li Zhang,et al.  Ligand Binding to Integrins* , 2000, The Journal of Biological Chemistry.

[33]  Le A. Trinh,et al.  The zebrafish bonnie and clyde gene encodes a Mix family homeodomain protein that regulates the generation of endodermal precursors. , 2000, Genes & development.

[34]  J. Duband,et al.  Adhesion and migration of avian neural crest cells on fibronectin require the cooperating activities of multiple integrins of the (beta)1 and (beta)3 families. , 1999, Journal of cell science.

[35]  D. Stainier,et al.  A molecular pathway leading to endoderm formation in zebrafish , 1999, Current Biology.

[36]  M. Komada,et al.  Hrs, a FYVE finger protein localized to early endosomes, is implicated in vesicular traffic and required for ventral folding morphogenesis. , 1999, Genes & development.

[37]  R. Hynes,et al.  Extensive Vasculogenesis, Angiogenesis, and Organogenesis Precede Lethality in Mice Lacking All αv Integrins , 1998, Cell.

[38]  D. Heinegård,et al.  Bone Matrix Proteins: Isolation and Characterization of a Novel Cell-binding Keratan Sulfate Proteoglycan (Osteoadherin) from Bovine Bone , 1998, The Journal of cell biology.

[39]  D. Cheresh,et al.  A Single Immunoglobulin-like Domain of the Human Neural Cell Adhesion Molecule L1 Supports Adhesion by Multiple Vascular and Platelet Integrins , 1997, The Journal of cell biology.

[40]  J. Sanes,et al.  Integrins mediate adhesion to agrin and modulate agrin signaling. , 1997, Development.

[41]  S. Neuhauss,et al.  Mutations affecting cell fates and cellular rearrangements during gastrulation in zebrafish. , 1996, Development.

[42]  M. S. Cooper,et al.  A cluster of noninvoluting endocytic cells at the margin of the zebrafish blastoderm marks the site of embryonic shield formation. , 1996, Developmental biology.

[43]  C. ffrench-Constant,et al.  A role in migration for the alpha V beta 1 integrin expressed on oligodendrocyte precursors , 1996 .

[44]  R. Hynes,et al.  Fibronectin receptor functions in embryonic cells deficient in alpha 5 beta 1 integrin can be replaced by alpha V integrins. , 1996, Molecular biology of the cell.

[45]  C. Kimmel,et al.  Specification of cell fates at the dorsal margin of the zebrafish gastrula. , 1996, Development.

[46]  C. Kimmel,et al.  Stages of embryonic development of the zebrafish , 1995, Developmental dynamics : an official publication of the American Association of Anatomists.

[47]  C. Damsky,et al.  Developmental regulation of integrin expression at the time of implantation in the mouse embryo. , 1993, Development.

[48]  L. Reichardt,et al.  Vitronectin and thrombospondin promote retinal neurite outgrowth: Developmental regulation and role of integrins , 1991, Neuron.

[49]  D. Traver,et al.  The zebrafish vitronectin receptor: characterization of integrin alphaV and beta3 expression patterns in early vertebrate development. , 2007, Developmental dynamics : an official publication of the American Association of Anatomists.

[50]  Lance A Davidson,et al.  Integrin alpha5beta1 and fibronectin regulate polarized cell protrusions required for Xenopus convergence and extension. , 2006, Current biology : CB.

[51]  I. Seiliez,et al.  Spatial and temporal expression of the zebrafish genome by large-scale in situ hybridization screening. , 2004, Methods in cell biology.

[52]  G. Nemerow,et al.  Integrin alpha(v)beta1 is an adenovirus coreceptor. , 2001, Journal of virology.

[53]  M. S. Cooper,et al.  Spatially distinct domains of cell behavior in the zebrafish organizer region. , 1997, Biochemistry and cell biology = Biochimie et biologie cellulaire.

[54]  K. Yamada,et al.  Differential mRNA regulation of integrin subunits alpha V, beta 1, beta 3, and beta 5 during mouse embryonic organogenesis. , 1995, Cell adhesion and communication.

[55]  L. Reichardt,et al.  Specific roles of the alpha V beta 1, alpha V beta 3 and alpha V beta 5 integrins in avian neural crest cell adhesion and migration on vitronectin. , 1994, Development.

[56]  B. Longest.  Sampling techniques. , 1971, Hospitals.