Adenosine triphosphatase in the uterus and duodenum of chicken hens during eggshell formation.

Plasma calcium concentration and uterine and duodenal adenosine triphosphatase (ATPase) activities were determined during shell formation for high (H) and low (L) shell strength lines of hens selected from the last of four consecutive generations. The H and L lines were divided into three groups according to shell formation at 0, 15, and 22 h following oviposition. Plasma total calcium was determined from blood samples collected from the common carotid artery. Activity of ATPase was determined in uterine and duodenal mucosa. Shell strength, shell weight, percentage of shell per egg and shell thickness of the H line hens significantly exceeded those of the L line. During shell formation, no significant fluctuation in plasma calcium levels was observed within a line, but overall mean plasma calcium concentrations were higher in the H line than L line. Uterine ATPase activity increased with time after oviposition in both lines, with that of the H line being greater. Duodenal ATPase activity of H line hens remained fairly constant throughout the period, but this value showed fluctuations in the L line hens. It thus appears that laying hens with high and low shell strength may vary in their ability to use calcium for shell formation.

[1]  M. Yamazaki,et al.  Time course of distribution of single dose oral or intravenous calcium-45 in laying hens. , 1986 .

[2]  D. Roland,et al.  Influence of dietary calcium on bone calcium utilization. , 1986, Poultry science.

[3]  E. Buss,et al.  Percent Shell and Plasma Mineral Concentrations in Three Strains of Domestic Fowl Selected for Thick or Thin Egg Shell Production , 1985 .

[4]  C. Tsang,et al.  Effects of vitamin D3 deficiency on adenosine triphosphatase activity of jejunums from white Leghorn hens. , 1984, Poultry science.

[5]  W. Coty,et al.  A high-affinity calcium-stimulated ATPase activity in the hen oviduct shell gland. , 1982, Archives of biochemistry and biophysics.

[6]  B. K. Thompson,et al.  Relationship Between Blood Ionized Calcium Levels and Shell Strength of Eggs Laid by White Leghorn Hens , 1981 .

[7]  G. Combs,et al.  Blood ionized calcium cycles in the chicken. , 1981, Poultry science.

[8]  B. K. Thompson,et al.  The Effects of Age and Strain on the Relationships between Destructive and Non-Destructive Measurements of Eggshell Strength for White Leghorn Hens , 1979 .

[9]  W. Eastin,et al.  On the mechanism of calcium secretion in the avian shell gland (uterus). , 1978, Biology of reproduction.

[10]  E. R. Miller,et al.  The relationship of production status to serum calcium and phosphorus in hens. , 1978, Poultry science.

[11]  S. Hurwitz,et al.  Calcium-binding protein and calcium absorption in the laying quail (Coturnix coturnix japonica). , 1976, Poultry science.

[12]  R. H. Alvarado,et al.  Ca-2+-Mg-2+-activated ATPase in the shell gland of japanese quail (Coturnix coturnix japonica). , 1975, Comparative biochemistry and physiology. B, Comparative biochemistry.

[13]  J. Garlich,et al.  Relationships between dietary and plasma concentrations of calcium and phosphorus in intact and ultimobranchialectomized chickens. , 1975, Poultry science.

[14]  C. Talbot,et al.  A study of the progressive deposition of shell in the shell gland of the domestic hen. , 1974, British poultry science.

[15]  M. Haussler,et al.  Induction of Intestinal Brush Border Alkaline Phosphatase by Vitamin D and Identity with Ca-ATPase , 1970, Nature.

[16]  R. Hodges.  pH and mineral ion levels in the blood of the laying hen (Gallus domesticus) in relation to egg shell formation , 1969 .

[17]  H. S. Paul,et al.  Dietary Calcium and Phosphorus and Variations in Plasma Alkaline Phosphatase Activity in Relationship to Physical Characteristics of Egg Shells , 1969 .

[18]  R. Schraer,et al.  Some Metabolic Aspects of Calcium Movement Across the Isolated Avian Shell Gland.∗ , 1967, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[19]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.