The complex fingerprint of vancomycin using electrochemical methods and mass spectrometry

Abstract Vancomycin (VAN) is a glycopeptide antibiotic, active against Gram-positive bacteria resistant to other antibiotics. VAN therefore needs to be monitored for maximum efficacy and minimum toxicity. Electrochemical sensors can be used for this purpose, using the direct electrochemical signal of VAN. Due to its molecular complexity and the need for analysis from complex matrices, the electrochemical detection of VAN requires the development of a complex electrochemical fingerprint (CEF), which covers a large variety of possible combinations of samples and interferents at concentrations of interest. The objective of this study was to develop a CEF for VAN, involving a battery of tests and the elucidation of the mechanism of anodic oxidation of VAN, using high-performance liquid chromatography coupled with mass-spectrometric analysis of the products obtained after small-scale electrolysis. A CEF for VAN was obtained, describing the electrochemical behavior of VAN at different commercially available screen-printed electrodes, with several electrolytes, considering successive analyses, after simple pretreatments, and examining both anodic and cathodic peaks. The concept was successfully applied to analysis of VAN in real samples and in the presence of some common interferents. Furthermore, a two-step combined electrochemical–chemical mechanism was proposed for the electrooxidation of VAN.

[1]  M. Jakubowska,et al.  Voltammetric classification of ciders with PLS-DA. , 2016, Talanta.

[2]  M. Hadi,et al.  Electroanalytical Determination of Vancomycin at a Graphene‐modified Electrode: Comparison of Electrochemical Property between Graphene, Carbon Nanotube, and Carbon Black , 2018, Electroanalysis.

[3]  Thanyarat Chaibun,et al.  Fingerprinting Green Curry: An Electrochemical Approach to Food Quality Control. , 2018, ACS sensors.

[4]  Nele Samyn,et al.  Electrochemical fingerprint of street samples for fast on-site screening of cocaine in seized drug powders , 2016, Chemical science.

[5]  Weijun Kang,et al.  Studies on the metabolism and degradation of vancomycin in simulated in vitro and aquatic environment by UHPLC-Triple-TOF-MS/MS , 2018, Scientific Reports.

[6]  F. Ibrahim,et al.  Stability-indicating spectrofluorimetric method with enhanced sensitivity for determination of vancomycin hydrochloride in pharmaceuticals and spiked human plasma: Application to degradation kinetics. , 2018, Journal of food and drug analysis.

[7]  Vikram Patel,et al.  Quality Assessment of U.S. Marketplace Vancomycin for Injection Products Using High-Resolution Liquid Chromatography-Mass Spectrometry and Potency Assays , 2012, Antimicrobial Agents and Chemotherapy.

[8]  Alexander L N van Nuijs,et al.  Cephalosporin Antibiotics: Electrochemical Fingerprints and Core Structure Reactions Investigated by LC-MS/MS. , 2019, Analytical chemistry.

[9]  A. Bruins,et al.  Electrochemical oxidation and cleavage of peptides analyzed with on-line mass spectrometric detection. , 2003, Rapid communications in mass spectrometry : RCM.

[10]  Ramasamy Venkatesan,et al.  Vancomycin bound biogenic gold nanoparticles: A different perspective for development of anti VRSA agents , 2011 .

[11]  R. Bischoff,et al.  Electrochemical oxidation and cleavage of tyrosine- and tryptophan-containing tripeptides. , 2010, Analytical chemistry.

[12]  L. Azzalis,et al.  The use of vancomycin with its therapeutic and adverse effects: a review. , 2015, European review for medical and pharmacological sciences.

[13]  Yu-Chie Chen,et al.  Antibacterial gold nanoparticle-based photothermal killing of vancomycin-resistant bacteria. , 2018, Nanomedicine.

[14]  Petr Solich,et al.  Modern methods for vancomycin determination in biological fluids by methods based on high-performance liquid chromatography--A review. , 2016, Journal of separation science.