Collagen as the Major Edible Component of Sea Cucumber (Stichopus japonicus)

: The body wall collagen of an edible sea cucumber, Stichopus japonicus, was studied with respect to its chemical composition and subunit structure. About 70% of the total body wall protein was accounted for by highly insoluble collagen fibers. The disaggregation with β-mercaptoethanol, 0.1 M NaOH treatment, and limited pepsin digestion of these collagen fibers resulted in complete solubilization. The solubilized collagen was isolated and characterized; it had 2 distinct subunits, αl and α2, which formed (α1)2α2 heterotrimers and was rich in glutamic acid when compared with other fibrillar collagens. The unique textural properties of cooked sea cucumber seem to be due to thermal denaturation of the insoluble collagen fibers.

[1]  T. Matsumura.  Collagen fibrils of the sea cucumber, Stichopus japonicus: purification and morphological study. , 1974, Connective tissue research.

[2]  T. Matsumura.  Shape, size and amino acid composition of collagen fibril of the starfish Asterias amurensis , 1973 .

[3]  Marc S. Lewis,et al.  The Chromatographic Separation and Amino Acid Composition of the Subunits of Several Collagens , 1963 .

[4]  N. Urano,et al.  Occurrence of fibrillar collagen with structure of (α1)2α2 in the test of sea urchin Asthenosoma ijimai , 1996 .

[5]  S. Kimura,et al.  Nereis cuticle collagen: isolation and characterization of two distinct subunits. , 1977, Biochemistry.

[6]  Xiao-ping Zhu,et al.  Characterization of Fish Muscle Type I Collagen , 1988 .

[7]  A. Keller,et al.  Proposed resolution of the paradox of extensive crosslinking and low tensile strength of cuvierian tubule collagen from the sea cucumber Holothuria forskali , 1982 .

[8]  Y. Kariya,et al.  Glycosaminoglycan involved in the cation-induced change of body wall structure of sea cucumber Stichopus japonicus. , 1990, Connective tissue research.

[9]  S. Kimura.  Determination of the glycosylated hydroxylysines in several invertebrate collagens. , 1972, Journal of biochemistry.

[10]  M. Reichlin,et al.  Antigenic relationships among fishes common to the Amazon River basin , 1979 .

[11]  T. Nagai,et al.  Partial characterization of collagen from purple sea urchin (Anthocidaris crassispina) test , 2000 .

[12]  M. Ishida,et al.  Molecular characterization of fibrillar collagen from the body wall of starfish Asterias amurensis , 1993 .