Acquired AmpC type beta-lactamases: an emerging problem in Italian long-term care and rehabilitation facilities.

We report the multiple detection of Proteus mirabilis isolates, from 4 different long-term care and rehabilitation facilities (LTCRFs) of Northern Italy, resistant to expanded-spectrum cephalosporins and cephamycins and producing an acquired ampC-like beta-lactamase, named CMY-16. Genotyping by PFGE showed that isolates were clonally related to each other, although not identical. In all isolates the bla(CMY16) gene was not transferable by conjugation and was found to be carried on the chromosome. These results revealed multifocal spreading of a CMY-16 producing P. mirabilis clone in Northern Italy and emphasize the emergence of similar acquired resistance determinants in the LTCRFs setting.

[1]  G. Rossolini,et al.  CMY-16, a Novel Acquired AmpC-Type β-Lactamase of the CMY/LAT Lineage in Multifocal Monophyletic Isolates of Proteus mirabilis from Northern Italy , 2006, Antimicrobial Agents and Chemotherapy.

[2]  Wonkeun Song,et al.  Plasmid-mediated, inducible AmpC beta-lactamase (DHA-1)-producing Enterobacteriaceae at a Korean hospital: wide dissemination in Klebsiella pneumoniae and Klebsiella oxytoca and emergence in Proteus mirabilis. , 2005, Diagnostic microbiology and infectious disease.

[3]  E. Bingen,et al.  First description of DHA-1 ampC β-lactamase in Proteus mirabilis , 2005 .

[4]  Allison M. Land,et al.  Molecular Characterization of Cefoxitin-Resistant Escherichia coli from Canadian Hospitals , 2005, Antimicrobial Agents and Chemotherapy.

[5]  E. Sadowy,et al.  Four Variants of the Citrobacter freundii AmpC-Type Cephalosporinases, Including Novel Enzymes CMY-14 and CMY-15, in a Proteus mirabilis Clone Widespread in Poland , 2004, Antimicrobial Agents and Chemotherapy.

[6]  V. Miriagou,et al.  CMY-13, a Novel Inducible Cephalosporinase Encoded by an Escherichia coli Plasmid , 2004, Antimicrobial Agents and Chemotherapy.

[7]  M. Inoue,et al.  CFE-1, a Novel Plasmid-Encoded AmpC β-Lactamase with an ampR Gene Originating from Citrobacter freundii , 2004, Antimicrobial Agents and Chemotherapy.

[8]  G. Jacoby,et al.  Epidemiology of Conjugative Plasmid-Mediated AmpC β-Lactamases in the United States , 2004, Antimicrobial Agents and Chemotherapy.

[9]  A. Philippon,et al.  Characterization of CMY-type beta-lactamases in clinical strains of Proteus mirabilis and Klebsiella pneumoniae isolated in four hospitals in the Paris area. , 2002, The Journal of antimicrobial chemotherapy.

[10]  G. Jacoby,et al.  Plasmid-determined AmpC-type beta-lactamases. , 2002, Antimicrobial agents and chemotherapy.

[11]  G. Arlet,et al.  Mise en évidence de la céphalosporinase plasmidique ACC-1 dans différentes entérobactéries (Klebsiella pneumoniae, Proteus mirabilis, Salmonella) isolées dans un hôpital tunisien (Sfax 1997–2000) , 2002 .

[12]  A. Philippon,et al.  [Identification of plasmid-encoded cephalosporinase ACC-1 among various enterobacteria (Klebsiella pneumoniae, Proteus mirabilis, Salmonella) isolated from a Tunisian hospital (Sfax 997-2000)]. , 2002, Pathologie-biologie.

[13]  M. Gomáriz,et al.  CMY-2-producing Salmonella enterica, Klebsiella pneumoniae, Klebsiella oxytoca, Proteus mirabilis and Escherichia coli strains isolated in Spain (October 1999-December 2000). , 2001, The Journal of antimicrobial chemotherapy.

[14]  P. Nordmann,et al.  Plasmid-Mediated Cephalosporinase ACC-1 in Clinical Isolates of Proteus mirabilis and Escherichia coli , 2000, European Journal of Clinical Microbiology and Infectious Diseases.

[15]  P. Nordmann,et al.  OXA-type beta-lactamases. , 1999, Current pharmaceutical design.

[16]  J. Pitout,et al.  β-Lactamases Responsible for Resistance to Expanded-Spectrum Cephalosporins in Klebsiella pneumoniae, Escherichia coli, and Proteus mirabilis Isolates Recovered in South Africa , 1998, Antimicrobial Agents and Chemotherapy.

[17]  D. Livermore.  beta-Lactamases in laboratory and clinical resistance , 1995, Clinical microbiology reviews.

[18]  G. Papanicolaou,et al.  Novel plasmid-mediated beta-lactamase (MIR-1) conferring resistance to oxyimino- and alpha-methoxy beta-lactams in clinical isolates of Klebsiella pneumoniae , 1990, Antimicrobial Agents and Chemotherapy.

[19]  R. Pearlman,et al.  Hybridization of nucleic acids directly in agarose gels. , 1983, Analytical biochemistry.