5′ capped and 3′ polyA-tailed sgRNAs enhance the efficiency of CRISPR-Cas9 system

[1]  A. Cheng,et al.  Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation and genomic labeling , 2016, Cell Research.

[2]  C. Bennett,et al.  Synthetic CRISPR RNA-Cas9–guided genome editing in human cells , 2015, Proceedings of the National Academy of Sciences.

[3]  J. Rinn,et al.  Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display , 2015, Nature Methods.

[4]  Israel Steinfeld,et al.  Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells , 2015, Nature Biotechnology.

[5]  Alexandro E. Trevino,et al.  Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex , 2014, Nature.

[6]  Fritz Eckstein,et al.  Phosphorothioates, essential components of therapeutic oligonucleotides. , 2014, Nucleic acid therapeutics.

[7]  Max A. Horlbeck,et al.  Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation , 2014, Cell.

[8]  J. Kieft,et al.  RNA structures that resist degradation by Xrn1 produce a pathogenic Dengue virus RNA , 2014, eLife.

[9]  Luke A. Gilbert,et al.  CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes , 2013, Cell.

[10]  James E. DiCarlo,et al.  RNA-Guided Human Genome Engineering via Cas9 , 2013, Science.

[11]  Le Cong,et al.  Multiplex Genome Engineering Using CRISPR/Cas Systems , 2013, Science.

[12]  G. Deleavey,et al.  Designing chemically modified oligonucleotides for targeted gene silencing. , 2012, Chemistry & biology.

[13]  J. Doudna,et al.  A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity , 2012, Science.

[14]  R. Terns,et al.  CRISPR-based adaptive immune systems. , 2011, Current opinion in microbiology.

[15]  J. Wilusz,et al.  Lsm proteins bind and stabilize RNAs containing 5′ poly(A) tracts , 2007, Nature Structural &Molecular Biology.