Genetics and genetic engineering ofZymomonas mobilis

Present knowledge on the genetics of the ethanologenic anaerobeZymomonas mobilis includes background information on: size, restriction, and to some extent hybridization, analysis of indigenous plasmids; mutagenesis and isolation of a wide variety of auxotrophic, drug resistant and conditional mutants; construction of shuttle cloning vectors able to replicate and express inZ. mobilis; development of gene transfer systems based on conjugal mobilization of plasmids fromEscherichia coli donors toZ. mobilis; expression of heterologous genes inZ. mobilis; cloning and analysis of genes encoding enzymes of the Entner-Doudoroff pathway. Moreover, preliminary data on recombinational repair mechanisms and plasmid stability, which are now available, makeZ. mobilis an attractive model system for molecular genetic research and, furthermore, they contribute towards expansion of the substrate and product range of this industrial microorganism.

[1]  Expression and Stability of a β-Glucosidase Gene of Ruminococcus albus in Zymomonas mobilis , 1989 .

[2]  L. Ingram,et al.  Promoter and nucleotide sequences of the Zymomonas mobilis pyruvate decarboxylase , 1987, Journal of bacteriology.

[3]  A. Afendra,et al.  Expression and stability of a recombinant plasmid in Zymomonas mobilis and Escherichia coli. , 1987, Journal of general microbiology.

[4]  L. Ingram,et al.  Genetic engineering of ethanol production in Escherichia coli , 1987, Applied and environmental microbiology.

[5]  Peter L. Rogers,et al.  R-Plasmid Transfer in Zymomonas mobilis , 1980, Applied and environmental microbiology.

[6]  J. Liu,et al.  Cloning, characterization and expression of the Zymononas mobilis eda gene that encodes 2‐keto‐3‐deoxy‐6‐phosphogluconate aldolase of the Entner‐Doudoroff pathway , 1991, Molecular microbiology.

[7]  R K Scopes,et al.  Gel electrophoretic analysis of Zymomonas mobilis glycolytic and fermentative enzymes: identification of alcohol dehydrogenase II as a stress protein , 1991, Journal of bacteriology.

[8]  L. Ingram,et al.  Biochemical and genetic improvement of Zymomonas mobilis , 1984 .

[9]  D. Helinski,et al.  Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[10]  Robert K. Scopes,et al.  Studies on cell-free metabolism: Ethanol production by extracts of Zymomonas mobilis , 1985 .

[11]  S. Park,et al.  Transfer ofBacillussubtilis endo-β-1,4-glucanase gene intoZymomonasanaerobia , 1988, Biotechnology Letters.

[12]  C. Drainas,et al.  Analysis of Natural Plasmids of Zymomonas mobilis ATCC 10988 , 1987 .

[13]  N. Misawa,et al.  Production of beta-carotene in Zymomonas mobilis and Agrobacterium tumefaciens by introduction of the biosynthesis genes from Erwinia uredovora , 1991, Applied and environmental microbiology.

[14]  L. Ingram,et al.  Cloning of the Zymomonas mobilis structural gene encoding alcohol dehydrogenase I (adhA): sequence comparison and expression in Escherichia coli , 1990, Journal of bacteriology.

[15]  P. Rogers,et al.  Isolation and characterization of Zymomonas mobilis plasmids , 1984 .

[16]  P. Greenfield,et al.  The production of ethanol from sucrose using Zymomonas mobilis , 1985, Applied Microbiology and Biotechnology.

[17]  D. Eveleigh,et al.  Genetic modification of Zymomonas mobilis. , 1990, Biotechnology advances.

[18]  H. Yanase,et al.  Construction of a Promoter-cloning Vector in Zymomonas mobilis , 1986 .

[19]  H. Sahm,et al.  Localisation of the glucose-fructose oxidoreductase in wild type and overproducing strains of Zymomonas mobilis , 1991 .

[20]  Katsumi Nakamura,et al.  Construction of a New Shuttle Vector forZymomonas mobilis , 1986 .

[21]  D. Eveleigh,et al.  Transfer of Plasmids to an Antibiotic-Sensitive Mutant of Zymomonas mobilis , 1986, Applied and environmental microbiology.

[22]  L. Ingram,et al.  Expression of a Lactose Transposon (Tn951) in Zymomonas mobilis , 1983, Applied and environmental microbiology.

[23]  P. Rogers,et al.  Kinetics of plasmid transformation in Zymomonas mobilis , 1988 .

[24]  N. Misawa,et al.  Three 1.7 Kilobase Pair Plasmids in Zymomonas mobilis NRRL B-806 , 1985 .

[25]  J Swings,et al.  The biology of Zymomonas , 1977, Bacteriological reviews.

[26]  H. Yanase,et al.  Fusion of spheroplasts and genetic recombination of Zymomonas mobilis. , 1985 .

[27]  T. Conway,et al.  Sequence and genetic organization of a Zymomonas mobilis gene cluster that encodes several enzymes of glucose metabolism , 1990, Journal of bacteriology.

[28]  N. Kurose,et al.  Occurrence of Plasmids in Zymomonas mobilis , 1982 .

[29]  J. Zucca,et al.  Sélection et analyse de mutants thermotolérants de Zymomonas mobilis, producteurs d'éthanol , 1985 .

[30]  L. Ingram,et al.  Glyceraldehyde-3-phosphate dehydrogenase gene from Zymomonas mobilis: cloning, sequencing, and identification of promoter region , 1987, Journal of bacteriology.

[31]  H. Stokes,et al.  Comparison of plasmids in strains of Zymomonas mobilis. , 1983, Plasmid.

[32]  P. Gunasekaran,et al.  Transfer and expression of an Erwinia chrysanthemi cellulase gene in Zymomonas mobilis , 1989 .

[33]  D. Eveleigh,et al.  Growth ofZymomonas mobilis CP4 on mannitol , 2004, Applied Microbiology and Biotechnology.

[34]  P. Rogers,et al.  Cloning and expression of a β-glucosidase gene from Xanthomonas albilineans in Escherichia coli and Zymomonas mobilis , 1989 .

[35]  H. Yanase,et al.  Expression of the Escherichia coli α-galactosidase and lactose permease genes in Zymomonas mobilis and its raffinose fermentation. , 1990 .

[36]  D. Deobagkar,et al.  A high molecular weight plasmid ofZymomonasmobilis harbours genes for HgCl2 resistance , 2005, Biotechnology Letters.

[37]  H. Doelle,et al.  The production of ethanol plus fructose sweetener using fructose utilization negative mutants of Zymomonas mobilis , 1986, Biotechnology Letters.

[38]  D. Tribe,et al.  Genetic alteration of Zymomonas mobilis for ethanol production. , 1982, Basic life sciences.

[39]  D. Eveleigh,et al.  Expression of an endoglucanase gene of Pseudomonas fluorescens var. cellulosa in Zymomonas mobilis , 1988 .

[40]  P. Rogers,et al.  Formation of ethanol from lactose by Zymomonas mobilis , 1984 .

[41]  P. Rogers,et al.  Zymomonas mobilis CP4: a clarification of strains via plasmid profiles , 1988 .

[42]  P. Rogers,et al.  Construction of a shuttle vector for Zymomonas mobilis , 1989, Applied Microbiology and Biotechnology.

[43]  Antonio H. Romano,et al.  d-Glucose Transport System of Zymomonas mobilis , 1985, Applied and environmental microbiology.

[44]  Robert R. Zall,et al.  Production of Acetaldehyde by Zymomonas mobilis , 1987, Applied and environmental microbiology.

[45]  K. Poralla,et al.  Effect of alcohols and temperature on the hopanoid content of Zymomonas mobilis , 1986, Applied Microbiology and Biotechnology.

[46]  L. Ingram,et al.  Phosphoglycerate kinase gene from Zymomonas mobilis: cloning, sequencing, and localization within the gap operon , 1988, Journal of bacteriology.

[47]  Transformation of Zymomonas mobilis with Plasmid DNA , 1986 .

[48]  H Sahm,et al.  Expression of an L-alanine dehydrogenase gene in Zymomonas mobilis and excretion of L-alanine , 1991, Applied and environmental microbiology.

[49]  N. Misawa,et al.  Nucleotide sequence of the 2.7 kb plasmid of Zymomonas mobilis ATCC10988 , 1989 .

[50]  L. Ingram,et al.  Expression Vector for Zymomonas mobilis , 1987, Applied and environmental microbiology.

[51]  T. Conway,et al.  Cloning, characterization, and nucleotide sequence analysis of a Zymomonas mobilis phosphoglucose isomerase gene that is subject to carbon source-dependent regulation , 1991, Journal of bacteriology.

[52]  P. Rogers,et al.  Minimal Medium for Isolation of Auxotrophic Zymomonas Mutants , 1982, Applied and environmental microbiology.

[53]  L. Ingram,et al.  Segmental message stabilization as a mechanism for differential expression from the Zymomonas mobilis gap operon , 1991, Journal of bacteriology.

[54]  J. L. Pond,et al.  Cloning, sequencing, and characterization of the principal acid phosphatase, the phoC+ product, from Zymomonas mobilis , 1989, Journal of bacteriology.

[55]  P. Gunasekaran,et al.  Cloning and sequencing of the sacA gene: characterization of a sucrase from Zymomonas mobilis , 1990, Journal of bacteriology.

[56]  P. Rogers,et al.  Transformation of Zymomonas mobilis by a hybrid plasmid. , 1984, Plasmid.

[57]  High frequency transformation of Zymomonas mobilis by plasmid DNA , 1987 .

[58]  G. Khachatourians,et al.  Isolation of Noninhibitory Strains of Zymomonas mobilis , 1985, Applied and environmental microbiology.

[59]  L. Ingram,et al.  Zymomonas mobilis Mutants with an Increased Rate of Alcohol Production , 1987, Applied and environmental microbiology.

[60]  N. Dunn,et al.  Expression of cloned Xanthomonasd-xylose catabolic genes in Zymomonas mobilis , 1988 .

[61]  P. Rogers,et al.  Behavior of the IncW plasmid Sa in Zymomonas mobilis. , 1987, Plasmid.

[62]  P. Gunasekaran,et al.  Cloning and expression in Escherichia coli of mercuric ion resistance coding genes from Zymomonas mobilis. , 1991, Journal of biotechnology.

[63]  L. Ingram,et al.  Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas mobilis , 1987, Journal of bacteriology.

[64]  Analysis and stability of Zymomonas mobilis ATCC 10988 plasmid pZMO3. , 1990, Plasmid.

[65]  L. Ingram,et al.  Expression of Zymomonas mobilis adhB (encoding alcohol dehydrogenase II) and adhB-lacZ operon fusions in recombinant Z. mobilis , 1989, Journal of bacteriology.

[66]  L. Ingram,et al.  Self-transmissible plasmid in Zymomonas mobilis carrying antibiotic resistance , 1984, Applied and environmental microbiology.

[67]  C. Drainas,et al.  Bioconversion of fruit and sugar beet extracts by Zymomonas mobilis to ethanol and other fine chemicals , 1989 .