Filtration of the Microaggregates in Stored Blood

Previous work suggests that pulmonary insufficiency after massive transfusion may be due to embolization of the microaggregates of platelets, leukocytes and amorphous material in stored blood. A Model T Coulter Counter® was used to measure the number and size of particles in stored blood from 10 to 164 μ Filtration of the stored blood through a standard infusion filter (170 μ pore) did not lower the particle counts. A 40 μ pore filter removed large (P < 0.001) but not medium or small particles. A Dacron wool filter significantly lowered particles in all size ranges. Simultaneous screen filtration pressure data showed that only Dacron wool effectively filtered the blood. When blood was centrifuged before being filtered, the weight of material removed by the 170 μ filter was more than doubled (P < 0.05) but that removed by Dacron wool was unchanged. The present studies stress the inefficiency of currently used blood filters and suggests that Dacron wool effectively filters stored blood.

[1]  R. Patterson,et al.  Disposable filter for microemboli. Use in cardiopulmonary bypass and massive transfusion. , 1971, JAMA.

[2]  J. Hill,et al.  Experience using a new dacron wool filter during extracorporeal circulation. , 1970, Archives of surgery.

[3]  J. Hill,et al.  Clinical use of a dacron wool filter during perfusion for open-heart surgery. , 1970, The Journal of thoracic and cardiovascular surgery.

[4]  J. Mcnamara,et al.  Screen filtration pressure in combat casualties. , 1970, Annals of surgery.

[5]  D. Doty,et al.  Death Associated with Multiple Pulmonary Emboli Soon after Battle Injury , 1970, Annals of surgery.

[6]  D. Doty,et al.  Changes in the Filtration Characteristics of Stored Blood , 1970, Annals of surgery.

[7]  R. Swank,et al.  Microvascular occlusion by platelet emboli after transfusion and shock , 1968 .

[8]  C. W. Walter,et al.  Safe transfusion apparatus. , 1966, Anesthesiology.

[9]  R. Swank,et al.  Screen filtration pressure and pulmonary hypertension. , 1965, The American journal of physiology.

[10]  D. Weiss,et al.  PLATELET MICROEMBOLI ASSOCIATED WITH MASSIVE BLOOD TRANSFUSION. , 1964, The American journal of pathology.

[11]  R. Swank.  Alteration of blood on storage: measurement of adhesiveness of "aging" platelets and leukocytes and their removal by filtration. , 1961, The New England journal of medicine.

[12]  H. Leveen,et al.  Polyolefin plastics as blood filters. , 1961, Surgery.

[13]  P. Mollison,et al.  A Note on Testing Filters in Blood Transfusion Sets , 1960 .

[14]  C. W. Walter,et al.  The mechanical factors responsible for rapid infusion of blood. , 1955, Surgery, gynecology & obstetrics.

[15]  W. B. Cooksey,et al.  Disposable, fine mesh filter for blood and plasma. , 1948, Journal of the American Medical Association.

[16]  Geo. H. Smith.  The Blood Bank and the Technique and Therapeutics of Transfusions , 1942, The Yale Journal of Biology and Medicine.

[17]  R. Lewisohn.  BLOOD PRESERVATION TECHNIC , 1938 .