NMR and X-Ray Investigation of the Phase Behavior of Phosphatidylethanolamines

Abstract The second moments of the proton magnetic resonance lines of DPPE and DMPE, below the main phase transition, vary within a similar range as those of the corresponding phosphatidylcholines. In contrast to the sharp “subtransition” between L[sgrave]’ and Lβ’ phase, observed in the latter class of lipids, both DMPE and DPPE exhibit a broad transition (∼15 K width) to a less mobile state at lower temperatures ∼ -20[ddot]C. The second moment of the hydrocarbon chain resonance line in this state is between the value observed in the L[sgrave]’ phase of DPPC and the higher value of the rigid lattice. In addition to these phenomena a time-dependent transition from the gel phase to a rigid crystalline state is observed in DMPE, with rigid lattice values for the second moments. This behaviour is presumably analogous to that found for DLPE. The X-ray diffraction patterns indicate that a hexagonal lattice exists for the hydrocarbon chains throughout the entire observed temperature range below the main transit...

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