A novel micro drill design based on Ros-DrillⒸ

This paper presents the development of a novel micro drill device for single living organisms. Currently, microinjection for mice and some other species is performed with the help of piezo-driven actuators with a very small amount of mercury column in the proximal end of the pipette in order to increase the success rate. However, the toxicity of mercury exhibits a risk factor both for the operator and the injected cells. Therefore, mercury-free devices have become a necessity. Here, a novel micro drill is developed based on the same principle of Ros-DrillⒸ piercing approach; piercing via rotational movements. The new drill is driven by a brushless motor, and it incorporates the micropipette holder. Both the amplitude and the frequency of rotational oscillations can be adjusted in very wide ranges. The experiments reveal that the drill is suitable for different tasks such as microinjection and biopsy of different organisms. It presents good performance in terms of success rate, ease of usage, compactness and compatibility with different manipulation systems.

[1]  Mingxuan Fan,et al.  Vibration study of the piezodriven pipettes immersed in viscous liquids , 2006 .

[2]  Clement Leung,et al.  Robotic ICSI (Intracytoplasmic Sperm Injection) , 2011, IEEE Transactions on Biomedical Engineering.

[3]  Thomas C. Evans,et al.  Transformation and microinjection , 2006 .

[4]  I. Wilmut,et al.  Sheep cloned by nuclear transfer from a cultured cell line , 1996, Nature.

[5]  R. Schultz,et al.  ICSI in the mouse. , 2010, Methods in enzymology.

[6]  A Onishi,et al.  Pig cloning by microinjection of fetal fibroblast nuclei. , 2000, Science.

[8]  Nejat Olgac,et al.  Zero magnitude error tracking control for servo system with extremely low-resolution digital encoder , 2017 .

[9]  O. Ueda,et al.  Application of the piezo-micromanipulator for injection of embryonic stem cells into mouse blastocysts. , 2001, Contemporary topics in laboratory animal science.

[10]  Yong Gan,et al.  A study of the zona piercing process in piezodriven intracytoplasmic sperm injection , 2008 .

[11]  Jürgen Götz,et al.  Pronuclear injection for the production of transgenic mice , 2007, Nature Protocols.

[12]  Nejat Olgaç,et al.  Microdynamics of the piezo-driven pipettes in ICSI , 2004, IEEE Transactions on Biomedical Engineering.

[13]  R Yanagimachi,et al.  Intracytoplasmic sperm injection in the mouse. , 1995, Biology of reproduction.

[14]  Peter C. Y. Chen,et al.  A micromanipulation system with dynamic force-feedback for automatic batch microinjection , 2007 .

[15]  A. Ergenc,et al.  A PMSM driven rotationally oscillating micro drill , 2017, 2017 10th International Conference on Electrical and Electronics Engineering (ELECO).

[16]  Paola Viganò,et al.  The ICSI procedure from past to future: a systematic review of the more controversial aspects. , 2015, Human reproduction update.

[17]  I. Chatterjee,et al.  Germline transformation of Caenorhabditis elegans by injection. , 2009, Methods in molecular biology.

[18]  Edward Grant,et al.  A Review of Automated Microinjection Systems for Single Cells in the Embryogenesis Stage , 2016, IEEE/ASME Transactions on Mechatronics.

[19]  Andrew Fire,et al.  Chapter 19 DNA Transformation , 1995 .

[20]  Zhenyu Zhang,et al.  An Adaptive Control Method for Ros-Drill Cellular Microinjector with Low-Resolution Encoder , 2013, Journal of medical engineering.

[21]  Dong Sun,et al.  A universal piezo-driven ultrasonic cell microinjection system , 2011, Biomedical microdevices.

[22]  Changhai Ru,et al.  The Development of Piezo-Driven Tools for Cellular Piercing , 2016 .

[23]  M. Rieckher,et al.  Generation of Caenorhabditis elegans Transgenic Animals by DNA Microinjection , 2017 .

[24]  Dong Sun,et al.  A Force Control Approach to a Robot-assisted Cell Microinjection System , 2010, Int. J. Robotics Res..

[25]  Ali Faut Ergenc.  A novel method for ICSI: Rotationally oscillating drill, design, control and monitoring , 2007 .

[26]  A. C. Perry,et al.  Piezo-actuated mouse intracytoplasmic sperm injection (ICSI) , 2007, Nature Protocols.

[27]  Min Tan,et al.  A Flexure-Guided Piezo Drill for Penetrating the Zona Pellucida of Mammalian Oocytes , 2018, IEEE Transactions on Biomedical Engineering.

[28]  Nejat Olgac,et al.  New technology for cellular piercing: rotationally oscillating μ-injector, description and validation tests , 2007, Biomedical microdevices.

[29]  Mehmet Toner,et al.  Rotationally oscillating drill (Ros‐Drill©) for mouse icsi without using mercury , 2008, Molecular reproduction and development.

[30]  Haibo Huang,et al.  Optimal design of a new structure piezo-driven cell injector , 2014, 14th IEEE International Conference on Nanotechnology.

[31]  Dong Sun,et al.  Single Cell Transfection through Precise Microinjection with Quantitatively Controlled Injection Volumes , 2016, Scientific Reports.

[32]  Moyers Michael.  International Electrotechnical Commission (IEC) Standards for Light Ion Beam Medical Electrical (ME) Equipment , 2017 .

[33]  Adem Çiçek,et al.  Vibration Analysis of Rolling Element Bearings Defects , 2014 .

[34]  S. J. Lacey,et al.  An Overview of Bearing Vibration Analysis , 2008 .