Microsystem for the immunomagnetic detection of Escherichia coli O157:H7

A hand held microsystem has been designed for the immunomagnetic detection and quantification of the pathogen Escherichia coli O157:H7 in food and clinical samples (see figure). In this work a technology that facilitates the integration of 3D microfluidic networks with any microfabricated biosensor to manufacture a Lab On a Chip has been developed. With this aim, the design of the microfluidic circuitry, the sensing film optimization, the packaging needed to carry out the assays in a safe and straightforward way and, finally, the development of the biological protocols involved in the measurements have been completed.

[1]  Anders Kristensen,et al.  Investigation of the dye concentration influence on the lasing wavelength and threshold for a micro-fluidic dye laser , 2004 .

[2]  M. Tijero,et al.  Novel three-dimensional embedded SU-8 microchannels fabricated using a low temperature full wafer adhesive bonding , 2004 .

[3]  Schreiber,et al.  Layered magnetic structures: Evidence for antiferromagnetic coupling of Fe layers across Cr interlayers. , 1986, Physical review letters.

[4]  A. Pühler,et al.  Detection and manipulation of biomolecules by magnetic carriers. , 2004, Journal of biotechnology.

[5]  A. Gehring,et al.  Enzyme-linked immunomagnetic electrochemical detection of live Escherichia coli 0157:H7 in apple juice. , 2005, Journal of food protection.

[6]  C. Milstein,et al.  Continuous cultures of fused cells secreting antibody of predefined specificity , 1975, Nature.

[7]  D. Kriz,et al.  Magnetic Transducers in Biosensors and Bioassays , 1999 .

[8]  Francis E. H. Tay,et al.  A novel micro-machining method for the fabrication of thick-film SU-8 embedded micro-channels , 2001 .

[9]  F J Blanco,et al.  Fabrication of SU-8 multilayer microstructures based on successive CMOS compatible adhesive bonding and releasing steps. , 2005, Lab on a chip.

[10]  George M. Whitesides,et al.  Composite ferromagnetic photoresist for the fabrication of microelectromechanical systems , 2005 .

[11]  A. Pühler,et al.  Comparison of a prototype magnetoresistive biosensor to standard fluorescent DNA detection. , 2004, Biosensors & bioelectronics.

[12]  F. Corsi,et al.  Verocytotoxin-producing Escherichia coli EH 0157:H7 colitis. , 2003, Journal of clinical gastroenterology.

[13]  J. Schotter,et al.  Magnetic particles as markers and carriers of biomolecules. , 2005, IEE proceedings. Nanobiotechnology.

[14]  A. Hütten,et al.  Mismatch-induced recrystallization of giant magneto-resistance (GMR) multilayer systems , 2007 .

[15]  G. Reiss,et al.  Evolution of the GMR-effect amplitude in copper/permalloy-multilayered thin films , 1999 .