Cell membrane chromatography coupled online with LC-MS method to screen anti-anaphylactoid components from Magnolia biondii Pamp. targeting on Mas-related G protein-coupled receptor X2.

Mas-related G protein-coupled receptor X2 was a mast cell specific receptor mediating anaphylactoid reactions by activating mast cells degranulation, it was also identified as a target for modulating mast cell-mediated anaphylactoid and inflammatory diseases. The anti-anaphylactoid drugs used clinically disturb the partial effect of partial mediators released by mast cells. The small molecule of Mas-related G protein-coupled receptor X2 specific antagonists may provide therapeutic action for the anaphylactoid and inflammatory diseases in the early stage. In this study, the Mas-related G protein-coupled receptor X2 high expression cell membrane chromatography was coupled online with LC-MS and successfully used to screen anti-anaphylactoid components from Magnolia biondii Pamp. Fargesin and Pinoresinol Dimethyl Ether were identified as potential anti-anaphylactoid components. Bioactivity of these two components were investigated by β hexosaminidase and histamine release assays on mast cells, and it was found that these two components could inhibit β hexosaminidase and histamine release in a concentration dependent manner. This Mas-related G protein-coupled receptor X2 high expression cell membrane chromatography coupled online with LC-MS system could be applied for screening potential anti-anaphylactoid components from natural medicinal herbs. This study also provided a powerful system for drug discovery in natural medicinal herbs. This article is protected by copyright. All rights reserved.