Fabrication and in vivo evaluation of nitric oxide-releasing electrochemical oxygen-sensing catheters.

Publisher Summary This chapter describes the procedure employed to construct functional NO-releasing catheter-type amperometric oxygen sensors, including coating the sensor with NO-release materials, assembly of the sensor itself, and in vivo evaluation of the analytical performance and hemocompatibility of the device. The procedure describing the specific fabrication of an intravascular catheter-type sensor that is introduced into an artery via a 14-gauge, 1.16-in. angiocath isdiscussed. In this procedure, the sensor is fixed into a four-way stopcock to allow a means to attach the sensor to the catheter securely and to introduce a saline drip to prevent blood from pooling in the catheter. The length and diameter of the sensor can be adjusted by selecting tubing of the appropriate diameters and adjusting lengths of wires and the sensor body to accommodate a wide variety of sizes and specific methods of introducing the sensor into the blood vessel or tubing to be monitored. Material and procedure for coating sensor sleeves, sensor fabrication, and concepts related to sensor calibration and use are also described.

[1]  R. Bartlett,et al.  Nitric oxide-releasing fumed silica particles: synthesis, characterization, and biomedical application. , 2003, Journal of the American Chemical Society.

[2]  Megan C Frost,et al.  Implantable chemical sensors for real-time clinical monitoring: progress and challenges. , 2002, Current opinion in chemical biology.

[3]  M. Meyerhoff,et al.  More lipophilic dialkyldiamine-based diazeniumdiolates: synthesis, characterization, and application in preparing thromboresistant nitric oxide release polymeric coatings. , 2003, Journal of medicinal chemistry.

[4]  S. Moncada,et al.  The anti‐aggregating properties of vascular endothelium: interactions between prostacyclin and nitric oxide , 1987, British journal of pharmacology.

[5]  C. K. Mahutte,et al.  On-line arterial blood gas analysis with optodes: current status. , 1998, Clinical biochemistry.

[6]  R. Puniyani,et al.  Hematological Aspects of Biocompatibility-Review Article , 1997, Journal of biomaterials applications.

[7]  M. Meyerhoff,et al.  Improving the thromboresistivity of chemical sensors via nitric oxide release: fabrication and in vivo evaluation of NO-releasing oxygen-sensing catheters. , 2000, Analytical chemistry.

[8]  R. Bartlett,et al.  Nitric oxide releasing silicone rubbers with improved blood compatibility: preparation, characterization, and in vivo evaluation. , 2002, Biomaterials.

[9]  Mark E Meyerhoff,et al.  In vivo biocompatibility and analytical performance of intravascular amperometric oxygen sensors prepared with improved nitric oxide-releasing silicone rubber coating. , 2002, Analytical chemistry.

[10]  G. Kojda,et al.  Vasoprotection by nitric oxide: mechanisms and therapeutic potential. , 2002, Cardiovascular research.

[11]  M. Meyerhoff.  In vivo blood-gas and electrolyte sensors: Progress and challenges , 1993 .

[12]  L. Ignarro,et al.  Evidence for the inhibitory role of guanosine 3', 5'-monophosphate in ADP-induced human platelet aggregation in the presence of nitric oxide and related vasodilators. , 1981, Blood.