VALIDATED SPECTROPHOTOMETRIC ESTIMATION OF LAMIVUDINE IN PURE AND TABLET DOSAGE FORM

Two simple and sensitive spectrophotometric methods have been developed for the estimation of Lamivudine in both pure and tablet dosage form. Methods A and B are based on the condensation reaction of Lamivudine with carbonyl reagents such as p-dimethylaminobenzaldehyde (PDAB) and vanillin in acidic condition to form yellow colored chromogen with absorption maxima at 476 nm and 474 nm respectively. Beer's law is valid in the concentration range of 2-10 µg/ml. This method was validated for precision, accuracy, ruggedness and robustness. Statistical analysis proves that the method is reproducible and selective for the estimation of the said drug.