Validation and reliability of the sex estimation of the human os coxae using freely available DSP2 software for bioarchaeology and forensic anthropology.

OBJECTIVES A new tool for skeletal sex estimation based on measurements of the human os coxae is presented using skeletons from a metapopulation of identified adult individuals from twelve independent population samples. For reliable sex estimation, a posterior probability greater than 0.95 was considered to be the classification threshold: below this value, estimates are considered indeterminate. By providing free software, we aim to develop an even more disseminated method for sex estimation. MATERIALS AND METHODS Ten metric variables collected from 2,040 ossa coxa of adult subjects of known sex were recorded between 1986 and 2002 (reference sample). To test both the validity and reliability, a target sample consisting of two series of adult ossa coxa of known sex (n = 623) was used. The DSP2 software (Diagnose Sexuelle Probabiliste v2) is based on Linear Discriminant Analysis, and the posterior probabilities are calculated using an R script. RESULTS For the reference sample, any combination of four dimensions provides a correct sex estimate in at least 99% of cases. The percentage of individuals for whom sex can be estimated depends on the number of dimensions; for all ten variables it is higher than 90%. Those results are confirmed in the target sample. DISCUSSION Our posterior probability threshold of 0.95 for sex estimate corresponds to the traditional sectioning point used in osteological studies. DSP2 software is replacing the former version that should not be used anymore. DSP2 is a robust and reliable technique for sexing adult os coxae, and is also user friendly.

[1]  Jana Velemínská,et al.  A Test of the DSP Sexing Method on CT Images from a Modern French Sample , 2015, Journal of forensic sciences.

[2]  E. Kranioti,et al.  Sex estimation of the Greek mandible with the aid of discriminant function analysis and posterior probabilities , 2014 .

[3]  R. Jantz,et al.  Fordisc 3 and Statistical Methods for Estimating Sex and Ancestry , 2012 .

[4]  Ó. Cambra‐Moo,et al.  Geometric morphometrics reveals restrictions on the shape of the female os coxae , 2017, Journal of anatomy.

[5]  Dennis C. Dirkmaat,et al.  A Companion to Forensic Anthropology: Dirkmaat/A Companion to Forensic Anthropology , 2012 .

[6]  Giancarlo Di Vella,et al.  Metric characterization of the human coxal bone on a recent Italian sample and multivariate discriminant analysis to determine sex. , 2012, Forensic science international.

[7]  L. Scheuer Application of osteology to forensic medicine , 2002, Clinical anatomy.

[8]  K. Quintelier Calcified uterine leiomyomata from a post-medieval nunnery in Brussels, Belgium , 2009 .

[9]  Varsha C. Pilbrow,et al.  Using CRANID to test the population affinity of known crania , 2012, Journal of anatomy.

[10]  D. Ubelaker,et al.  Standards for Data Collection from Human Skeletal Remains , 1994 .

[11]  Julie Josse,et al.  Handling missing values in exploratory multivariate data analysis methods , 2012 .

[12]  Daniel Franklin,et al.  Estimation of sex from cranial measurements in a Western Australian population. , 2013, Forensic science international.

[13]  Summer J Decker,et al.  A Transparent Method for Sex Estimation Using Refined DSP Measurements of the Innominate , 2015 .

[14]  V. Mariotti,et al.  Recording Specific Entheseal Changes of Fibrocartilaginous Entheses: Initial Tests Using the Coimbra Method , 2013 .

[15]  R Core Team,et al.  R: A language and environment for statistical computing. , 2014 .

[16]  Nicholas V. Passalacqua,et al.  Sex determination of human skeletal populations using latent profile analysis. , 2013, American journal of physical anthropology.

[17]  V. Sládek,et al.  Human manipulative behavior in the Central European Late Eneolithic and Early Bronze Age: humeral bilateral asymmetry. , 2007, American journal of physical anthropology.

[18]  E. Cunha,et al.  DSP: A tool for probabilistic sex diagnosis using worldwide variability in hip-bone measurements , 2005 .

[19]  Jaroslav Bruzek,et al.  A method for visual determination of sex, using the human hip bone. , 2002, American journal of physical anthropology.

[20]  Eric Rivals,et al.  The ancient Yakuts: a population genetic enigma , 2015, Philosophical Transactions of the Royal Society B: Biological Sciences.

[21]  P. Courtaud,et al.  In situ study of the Gravettian individual from Cussac cave, locus 2 (Dordogne, France). , 2015, American journal of physical anthropology.

[22]  E. Kranioti,et al.  Sexual dimorphism of the metacarpals in contemporary Cretans: Are there differences with mainland Greeks? , 2015, Forensic science international.

[23]  Lia Betti,et al.  Sexual dimorphism in the size and shape of the os coxae and the effects of microevolutionary processes. , 2014, American journal of physical anthropology.

[24]  T. D. Stewart,et al.  Sex determination of the skeleton by guess and by measurement. , 1954, American journal of physical anthropology.

[25]  Nicole M. Scott,et al.  Intraobserver error associated with measurements of the hand , 2005, American journal of human biology : the official journal of the Human Biology Council.

[26]  D. Dirkmaat,et al.  Forensic Anthropology: Embracing the New Paradigm , 2012 .

[27]  L. Hager Sex differences in the sciatic notch of great apes and modern humans. , 1996, American journal of physical anthropology.

[28]  F. P. Schulter-Ellis,et al.  Determination of sex with a discriminant analysis of new pelvic bone measurements: Part I. , 1983, Journal of forensic sciences.

[29]  Angi M Christensen,et al.  Evidentiary Standards for Forensic Anthropology * , 2009, Journal of forensic sciences.

[30]  L. E. Hoyme The earliest use of indices for sexing pelves. , 1957, American journal of physical anthropology.

[31]  Mario A. Apostol,et al.  Sexual dimorphism of the tibia in contemporary Greeks, Italians, and Spanish: forensic implications , 2015, International Journal of Legal Medicine.

[32]  Jaroslav Bruzek,et al.  Methodology and Reliability of Sex Determination From the Skeleton , 2006 .

[33]  Schull Wj,et al.  Sex determination from the skeleton. , 1957 .

[34]  Aline Thomas Bioarchaeology of the middle Neolithic: evidence for archery among early European farmers. , 2014, American journal of physical anthropology.

[35]  J. Dupej,et al.  Disregarding population specificity: its influence on the sex assessment methods from the tibia , 2016, International Journal of Legal Medicine.

[36]  Victor Sholukha,et al.  Sex determination using the Probabilistic Sex Diagnosis (DSP: Diagnose Sexuelle Probabiliste) tool in a virtual environment. , 2014, Forensic science international.

[37]  Santiago Genovés L'estimation des différences sexuelles dans l'os coxal : différences métriques et différences morphologiques , 1959 .

[38]  Laura A B Wilson,et al.  Shape, size, and maturity trajectories of the human ilium. , 2015, American journal of physical anthropology.

[39]  S. Washburn Sex differences in the pubic bone. , 1948, American journal of physical anthropology.

[40]  F. P. Schulter-Ellis,et al.  Determination of sex with a discriminant analysis of new pelvic bone measurements: Part II. , 1985, Journal of forensic sciences.

[41]  Gérald Quatrehomme,et al.  Sex determination using the DSP (probabilistic sex diagnosis) method on the coxal bone: Efficiency of method according to number of available variables. , 2017, Forensic science international.

[42]  Phillip L Walker,et al.  Sexing skulls using discriminant function analysis of visually assessed traits. , 2008, American journal of physical anthropology.

[43]  Debra A Komar,et al.  Manufactured populations: what do contemporary reference skeletal collections represent? A comparative study using the Maxwell Museum documented collection. , 2008, American journal of physical anthropology.

[44]  J. Gaillard Détermination sexuelle d'un os coxal fragmentaire. , 1960 .

[45]  K. Rosenberg,et al.  Birth, obstetrics and human evolution , 2002, BJOG : an international journal of obstetrics and gynaecology.

[46]  R. Jantz,et al.  The Remarkable Change in Euro-American Cranial Shape and Size , 2016, Human Biology.

[47]  Jaroslav Bruzek,et al.  Inter- and intra-observer error in pelvic measurements and its implication for the methods of sex determination , 1994 .

[48]  Jennifer M Vollner,et al.  A revised method of sexing the human innominate using Phenice's nonmetric traits and statistical methods. , 2012, American journal of physical anthropology.

[49]  M. Richards,et al.  Multi-isotopic analysis reveals individual mobility and diet at the Early Iron Age monumental tumulus of Magdalenenberg, Germany. , 2012, American journal of physical anthropology.

[50]  Julie Josse,et al.  Principal component analysis with missing values: a comparative survey of methods , 2015, Plant Ecology.

[51]  Michael W. Kenyhercz,et al.  Sexual dimorphism in cranial morphology among modern South Africans , 2015, International Journal of Legal Medicine.

[52]  M Steyn,et al.  An assessment of the repeatability of pubic and ischial measurements. , 2012, Forensic science international.