Optimization of Phenolics and Flavonoids Extraction Conditions and Antioxidant Activity of Roasted Yerba-Mate Leaves (Ilex paraguariensis A. St.-Hil., Aquifoliaceae) using Response Surface Methodology.

This study focused on maximizing the extraction of total phenolics and flavonoids as well as the antioxidant activity measured by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay from roasted yerba mate (Ilex paraguariensis) as a function of time (5, 7.5 and 10 min) and temperature of extraction (60, 75 and 90°C). The data were subjected to Response Surface Methodology and the results showed that polynomial equations were significant, did not present lack of fit, and presented adjusted determination coefficients above 98%, proving their suitability for prediction purposes. Using the desirability function, the optimum operating conditions to obtain a higher extraction of antioxidants was found to be 10 min of extraction at 90°C, and the tea prepared under these experimental conditions presented 427.74 mg of gallic acid equivalents per liter and 80.02% of inhibition of the DPPH radical. The flavonoid content was highly correlated (r = 0.9046, p < 0.001) to the antioxidant capacity.

[1]  N. Seeram,et al.  Comparison of antioxidant potency of commonly consumed polyphenol-rich beverages in the United States. , 2008, Journal of agricultural and food chemistry.

[2]  L. Owczarek,et al.  Juices and beverages with a controlled phenolic content and antioxidant capacity , 2004 .

[3]  C. Soccol,et al.  Development of an Innovative Nutraceutical Fermented Beverage from Herbal Mate (Ilex paraguariensis A.St.-Hil.) Extract , 2012, International journal of molecular sciences.

[4]  A. Gugliucci,et al.  Protective action of Ilex paraguariensis extract against free radical inactivation of paraoxonase-1 in high-density lipoprotein. , 2007, Planta medica.

[5]  Daniel Granato,et al.  Influence of passion fruit juice on colour stability and sensory acceptability of non-sugar Yacon-based pastes , 2011 .

[6]  Wenyan Zhou,et al.  Evaluation of antitumour activity of tea carbohydrate polymers in hepatocellular carcinoma animals. , 2012, International journal of biological macromolecules.

[7]  Douglas C. Montgomery,et al.  Response Surface Methodology: Process and Product Optimization Using Designed Experiments , 1995 .

[8]  N. Soares,et al.  Modelling Bacillus cereus adhesion on stainless steel surface as affected by temperature, pH and time , 2014 .

[9]  M. C. Lanari,et al.  Polyphenol input to the antioxidant activity of yerba mate (Ilex paraguariensis) extracts , 2012 .

[10]  Bo Li,et al.  Response surface optimization of supercritical fluid extraction of kaempferol glycosides from tea seed cake , 2010 .

[11]  J. Sudji,et al.  Effects of Different Extraction Methods and Conditions on the Phenolic Composition of Mate Tea Extracts , 2012, Molecules.

[12]  Daniel Granato,et al.  Physical stability assessment and sensory optimization of a dairy-free emulsion using response surface methodology. , 2010, Journal of food science.

[13]  M. Ribeiro,et al.  Effects of mate tea (Ilex paraguariensis) ingestion on mRNA expression of antioxidant enzymes, lipid peroxidation, and total antioxidant status in healthy young women. , 2009, Journal of agricultural and food chemistry.

[14]  A. Gugliucci,et al.  Antioxidant activity of a botanical extract preparation of Ilex paraguariensis: prevention of DNA double-strand breaks in Saccharomyces cerevisiae and human low-density lipoprotein oxidation. , 2003, Journal of alternative and complementary medicine.

[15]  M. Berhow,et al.  Mate (Ilex paraguariensis St. Hilaire) saponins induce caspase-3-dependent apoptosis in human colon cancer cells in vitro , 2011 .

[16]  Mengcheng Tang,et al.  The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals , 1999 .

[17]  Daniel Granato,et al.  A comparative study of the phenolic compounds and the in vitro antioxidant activity of different Brazilian teas using multivariate statistical techniques , 2014 .

[18]  R. N. Cavalcanti,et al.  Processing optimization of probiotic yogurt containing glucose oxidase using response surface methodology. , 2010, Journal of dairy science.

[19]  Cristina Giatti Marques de Souza,et al.  Chá verde brasileiro (Camellia sinensis var assamica): efeitos do tempo de infusão, acondicionamento da erva e forma de preparo sobre a eficiência de extração dos bioativos e sobre a estabilidade da bebida , 2010 .

[20]  Daniel Granato,et al.  Phenolic composition of South American red wines classified according to their antioxidant activity, retail price and sensory quality. , 2011, Food chemistry.

[21]  Chunming Liu,et al.  Application of response surface methodology to optimise supercritical carbon dioxide extraction of oil from rapeseed (Brassica napus L.) , 2012 .

[22]  Daniel Granato,et al.  Development and sensory profile of a probiotic beverage from apple fermented with Lactobacillus casei , 2012 .

[23]  M. Ribeiro,et al.  Effect of mate tea (Ilex paraguariensis) supplementation on oxidative stress biomarkers and LDL oxidisability in normo- and hyperlipidaemic humans , 2011 .

[24]  L. A. Brumovsky,et al.  A novel procedure to measure the antioxidant capacity of Yerba maté extracts , 2012 .

[25]  A. Gambero,et al.  Anti-inflammatory effects of yerba maté extract (Ilex paraguariensis) ameliorate insulin resistance in mice with high fat diet-induced obesity , 2011, Molecular and Cellular Endocrinology.

[26]  Ji-rong Song,et al.  Investigating the antioxidant mechanism of violacein by density functional theory method , 2007 .

[27]  A. Gugliucci,et al.  Recent advances on Ilex paraguariensis research: minireview. , 2011, Journal of ethnopharmacology.

[28]  Fabiana Lemos Goularte Dutra,et al.  Determinação de compostos fenólicos por cromatografia líquida de alta eficiência isocrática durante estacionamento da erva-mate , 2010 .

[29]  Daniel Granato,et al.  Sensory evaluation and physicochemical optimisation of soy-based desserts using response surface methodology , 2010 .

[30]  Daniel Granato,et al.  Observations on the use of statistical methods in Food Science and Technology , 2014 .

[31]  E. D. de Mejia,et al.  Yerba mate tea (Ilex paraguariensis): Phenolics, antioxidant capacity and in vitro inhibition of colon cancer cell proliferation , 2010 .

[32]  C. Berset,et al.  Use of a Free Radical Method to Evaluate Antioxidant Activity , 1995 .

[33]  Deniz Baş,et al.  Modeling and optimization I: Usability of response surface methodology , 2007 .

[34]  G. Derringer,et al.  Simultaneous Optimization of Several Response Variables , 1980 .

[35]  G. Schinella,et al.  Antioxidant and cardioprotective effects of Ilex brasiliensis: A comparative study with Ilex paraguariensis (yerba mate) , 2009 .

[36]  V. L. Singleton,et al.  Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents , 1965, American Journal of Enology and Viticulture.

[37]  F. Lajolo,et al.  Flavonoids, total phenolics and antioxidant capacity: comparison between commercial green tea preparations , 2010 .

[38]  M. Salvador,et al.  Chemical composition and antioxidant activity of yerba-mate (Ilex paraguariensis A.St.-Hil., Aquifoliaceae) extract as obtained by spray drying. , 2011, Journal of agricultural and food chemistry.

[39]  M. Berhow,et al.  Yerba mate (Ilex paraguariensis St. Hilaire) saponins inhibit human colon cancer cell proliferation , 2012 .

[40]  G. Aldini,et al.  Characterization of phenolic antioxidants from Maté (Ilex Paraguayensis) by liquid chromatography/mass spectrometry and liquid chromatography/tandem mass spectrometry , 1998 .

[41]  D. Granato,et al.  Inactivation of Neosartorya fischeri and Paecilomyces variotii on paperboard packaging material by hydrogen peroxide and heat , 2012 .