Conductometric formaldehyde sensitive biosensor with specifically adapted analytical characteristics

A conductometric enzyme biosensor for determination of formaldehyde in aqueous solutions has been developed using interdigitated thin-film planar electrodes and immobilised alcohol oxidase from Hansenula polymorpha . The biosensor steady-state response was reached after about 1 min. Its dynamic range can vary from 0.05 to 500 mM formaldehyde and depends on the time of enzymatic membrane cross-linking by glutaraldehyde and on the buffer concentration used. The biosensor developed was not completely specific and selective. It demonstrated no response to primary alcohols and other substrates alone. Unfortunately, the response of this biosensor in a mixture of formaldehyde and methanol was decreased in comparison to the one observed for pure formaldehyde, even if no response was obtained with the interfering specie alone. The operational stability was not <20 h and the relative standard deviation appeared to be about 3%. Moreover, the storage stability was more than 1 month. © 2001 Elsevier Science B.V. All rights reserved.

[1]  Rolf Schmid,et al.  Biosensors: Applications in Medicine, Environmental Protection, and Process Control , 1989 .

[2]  J C Septon,et al.  Workplace air sampling and polarographic determination of formaldehyde. , 1982, American Industrial Hygiene Association journal.

[3]  G. S. Wilson,et al.  Electrochemical biosensors: recommended definitions and classification. , 2001, Biosensors & bioelectronics.

[4]  Mary Lynn Grayeski,et al.  New chemiluminescent derivatizing agent for the analysis of aldehydes and ketones by high-performance liquid chromatography with peroxyoxalate chemiluminescence , 1987 .

[5]  Nicole Jaffrezic-Renault,et al.  Conductometric biosensors based on cholinesterases for sensitive detection of pesticides , 1994 .

[6]  C. Martelet,et al.  Development of highly selective and stable potentiometric sensors for formaldehyde determination. , 2000, Biosensors & bioelectronics.

[7]  G. R. Möhlmann,et al.  Formaldehyde Detection in Air by Laser-Induced Fluorescence , 1985 .

[8]  I. J. van der Klei,et al.  Alcohol oxidase from Hansenula polymorpha CBS 4732. , 1990, Methods in enzymology.

[9]  A. A. Shul'ga,et al.  Thin-film conductometric biosensors for glucose and urea determination. , 1994, Biosensors & bioelectronics.

[10]  J. Woodward,et al.  Stabilization of analytical enzymes using a novel polymer–carbohydrate system and the production of a stabilized, single reagent for alcohol analysis , 1992 .

[11]  M. Ho,et al.  Enzymatic method for the determination of formaldehyde , 1990 .

[12]  N. Cade,et al.  Electrochemical enzyme sensor for formaldehyde operating in the gas phase , 1996 .

[13]  Malcolm J. Whiting,et al.  Falsely low estimation of triglycerides in lipemic plasma by the enzymatic triglyceride method with modified Trinder's chromogen. , 1990, Clinical chemistry.

[14]  G. Moores,et al.  Conductimetry in enzyme studies. , 1972, European journal of biochemistry.

[15]  M. Flyvholm,et al.  Identification of formaldehyde releasers and occurrence of formaldehyde and formaldehyde releasers in registered chemical products. , 1993, American journal of industrial medicine.

[16]  Wing-Hong Chan,et al.  Adsorption voltammetric determination of μg 1−1 levels formaldehyde via in situ derivatization with Girard's reagent T , 1997 .

[17]  Purnendu K. Dasgupta,et al.  Solubility of gaseous formaldehyde in liquid water and generation of trace standard gaseous formaldehyde. , 1986, Environmental science & technology.

[18]  A. Soldatkin,et al.  A Novel Enzyme Biosensor Specific for Formaldehyde Based on pH‐Sensitive Field Effect Transistors , 1997 .

[19]  T. Dumas,et al.  Determination of formaldehyde in air by gas chromatography , 1982 .

[20]  Barry Dellinger,et al.  Sampling and ion chromatographic determination of formaldehyde and acetaldehyde , 1981 .

[21]  E. Cotsaris,et al.  Low level determination of formaldehyde in water by high-performance liquid chromatography , 1993 .

[22]  P. Patnaik.  Handbook of Environmental Analysis: Chemical Pollutants in Air, Water, Soil, and Solid Wastes , 1997 .

[23]  A. El'skaya,et al.  A cell biosensor specific for formaldehyde based on pH-sensitive transistors coupled to methylotrophic yeast cells with genetically adjusted metabolism. , 1993, Analytical biochemistry.

[24]  D. Pletcher,et al.  A microelectrode study of the reduction of formaldehyde in neutral concentrated aqueous solutions , 1990 .

[25]  Marina Scarpa,et al.  Potentiometric detection of formaldehyde in air by an aldehyde dehydrogenase FET , 1996 .

[26]  Nicole Jaffrezic-Renault,et al.  Glucose sensitive conductometric biosensor with additional Nafion membrane: reduction of influence of buffer capacity on the sensor response and extension of its dynamic range , 1994 .

[27]  J. Hart,et al.  Development of a disposable ethanol biosensor based on a chemically modified screen-printed electrode coated with alcohol oxidase for the analysis of beer. , 2000, Biosensors & bioelectronics.

[28]  Liming Sun,et al.  Determination of nano-molar levels of formaldehyde in drinking water using flow-injection system with immobilized formaldehyde dehydrogenase after off-line solid-phase extraction , 1999 .