The effect of plant hormone gibberellic acid on germination indices Secale montanum in vitro and pot experiments under drought conditions

One of the effects of drought is the reduction of germination, seedling establishment and seedling growth. Pretreatment improves some aspects of seed germination and seedling establishment. To investigation the effect of pretreatment with Gibberellic acid on the seed germination of Secale Montanum in stages of growth in vitro and pot under drought stress conditions experiment was conducted in laboratory and greenhouse Tehran University. Experiments design in factorial randomized complete block with three replications. Treatments examination included two levels of priming at 10 and 15 °C (first factor), Gibberellic acid treatments on three levels: 50, 100 and 150 ppm for 12 h (second factor) and osmotic potential (PEG 6000) in three 10 -, 12 - and 14 - times (factor III), respectively. The results showed that pretreatment with Gibberellic acid of seed in drought stress conditions in vitro increased the germination percentage, germination rate, root length and shoot length and also in medium pot, seedling establishment percentage, seedling establishment rate, root length and shoot length as influenced by different hormonal treatments, with Gibberellic acid 50 ppm at 10 °C and Gibberellic acid 100 ppm at 10 °C and - 10 times surface in the experimental vitro and pot have had the most positive effect on germination and growth factors, respectively. The seed priming treatment at 10 °C compared with 15 °C displayed a better outcome.