Interactions of engineered nanomaterials in physiological media and implications for in vitro dosimetry

Abstract In vitro toxicity assays are efficient and inexpensive tools for screening the increasing number of engineered nanomaterials (ENMs) entering the consumer market. However, the data produced by in vitro studies often vary substantially among different studies and from in vivo data. In part, these discrepancies may be attributable to lack of standardisation in dispersion protocols and inadequate characterisation of particle–media interactions which may affect the particle kinetics and the dose delivered to cells. In this study, a novel approach for preparation of monodisperse, stabilised liquid suspensions is presented and coupled with a numerical model which estimates delivered dose values. Empirically derived material- and media-specific functions are presented for each media–ENM system that can be used to convert administered doses to delivered doses. The interactions of ENMs with a variety of physiologic media were investigated and the importance of this approach was demonstrated by in vitro cytotoxicity assays using THP-1 macrophages.

[1]  Mark R. Wiesner,et al.  Ultrasonic dispersion of nanoparticles for environmental, health and safety assessment – issues and recommendations , 2011, Nanotoxicology.

[2]  A. Imrich,et al.  Heterogeneity in Macrophage Phagocytosis of Staphylococcus aureus Strains: High-Throughput Scanning Cytometry-Based Analysis , 2009, PloS one.

[3]  J. Brain,et al.  Biologic responses to nanomaterials depend on exposure, clearance, and material characteristics , 2009 .

[4]  B. Lehnert,et al.  Correlation between particle size, in vivo particle persistence, and lung injury. , 1994, Environmental health perspectives.

[5]  Li Wei,et al.  Sharper and faster "nano darts" kill more bacteria: a study of antibacterial activity of individually dispersed pristine single-walled carbon nanotube. , 2009, ACS nano.

[6]  R. Amal,et al.  Stabilization of magnetic iron oxide nanoparticles in biological media by fetal bovine serum (FBS). , 2011, Langmuir : the ACS journal of surfaces and colloids.

[7]  A. Imrich,et al.  Signaling pathways required for macrophage scavenger receptor-mediated phagocytosis: analysis by scanning cytometry , 2008, Respiratory research.

[8]  Vicki Stone,et al.  Toxicology of nanoparticles: A historical perspective , 2007 .

[9]  Francesco Stellacci,et al.  Effect of surface properties on nanoparticle-cell interactions. , 2010, Small.

[10]  G. Sotiriou,et al.  A novel platform for pulmonary and cardiovascular toxicological characterization of inhaled engineered nanomaterials , 2012, Nanotoxicology.

[11]  V. Grassian,et al.  Agglomeration, isolation and dissolution of commercially manufactured silver nanoparticles in aqueous environments , 2009 .

[12]  M. Greenberg,et al.  Toxicity Testing in the 21st Century , 2009, Risk analysis : an official publication of the Society for Risk Analysis.

[13]  Lutz Mädler,et al.  Nanomaterials in the environment: from materials to high-throughput screening to organisms. , 2011, ACS nano.

[14]  P. Biswas,et al.  Concept of Assessing Nanoparticle Hazards Considering Nanoparticle Dosemetric and Chemical/Biological Response Metrics , 2010, Journal of toxicology and environmental health. Part A.

[15]  A. Nel,et al.  Self-organizing map analysis of toxicity-related cell signaling pathways for metal and metal oxide nanoparticles. , 2011, Environmental science & technology.

[16]  Jeffrey I. Zink,et al.  Dispersion and stability optimization of TiO2 nanoparticles in cell culture media. , 2010, Environmental science & technology.

[17]  Mihail C. Roco,et al.  Nanotechnology Research Directions for Societal Needs in 2020: Retrospective and Outlook , 2011 .

[18]  T. Sandström,et al.  Adverse cardiovascular effects of air pollution , 2009, Nature Clinical Practice Cardiovascular Medicine.

[19]  T. Xia,et al.  Toxic Potential of Materials at the Nanolevel , 2006, Science.

[20]  Joel G Pounds,et al.  ISDD: A computational model of particle sedimentation, diffusion and target cell dosimetry for in vitro toxicity studies , 2010, Particle and Fibre Toxicology.

[21]  Joel G Pounds,et al.  Particokinetics in vitro: dosimetry considerations for in vitro nanoparticle toxicity assessments. , 2007, Toxicological sciences : an official journal of the Society of Toxicology.

[22]  S. Pratsinis,et al.  Development and characterization of a Versatile Engineered Nanomaterial Generation System (VENGES) suitable for toxicological studies , 2010, Inhalation toxicology.

[23]  Clinton F Jones,et al.  In vitro assessments of nanomaterial toxicity. , 2009, Advanced drug delivery reviews.

[24]  Bengt Fadeel,et al.  Better safe than sorry: Understanding the toxicological properties of inorganic nanoparticles manufactured for biomedical applications. , 2010, Advanced drug delivery reviews.

[25]  D. Laxen.  A specific conductance method for quality control in water analysis , 1977 .

[26]  M. C. Sterling,et al.  Application of fractal flocculation and vertical transport model to aquatic sol-sediment systems. , 2005, Water research.

[27]  Kenneth A. Dawson,et al.  Nanoparticle size and surface properties determine the protein corona with possible implications for biological impacts , 2008, Proceedings of the National Academy of Sciences.

[28]  Saber M Hussain,et al.  Characterization of nanomaterial dispersion in solution prior to in vitro exposure using dynamic light scattering technique. , 2008, Toxicological sciences : an official journal of the Society of Toxicology.

[29]  Pratim Biswas,et al.  Characterization of size, surface charge, and agglomeration state of nanoparticle dispersions for toxicological studies , 2009 .

[30]  Kent E. Pinkerton,et al.  Meeting Report: Hazard Assessment for Nanoparticles—Report from an Interdisciplinary Workshop , 2007, Environmental health perspectives.

[31]  Conrad Coester,et al.  Particle and Fibre Toxicology BioMed Central Methodology , 2008 .

[32]  K. Wittmaack.  In Search of the Most Relevant Parameter for Quantifying Lung Inflammatory Response to Nanoparticle Exposure: Particle Number, Surface Area, or What? , 2006, Environmental health perspectives.

[33]  Hak Soo Choi,et al.  Rapid translocation of nanoparticles from the lung airspaces to the body , 2010, Nature Biotechnology.

[34]  David Y Lai,et al.  Toward toxicity testing of nanomaterials in the 21st century: a paradigm for moving forward. , 2012, Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology.

[35]  G. Oberdörster,et al.  Nanotoxicology: An Emerging Discipline Evolving from Studies of Ultrafine Particles , 2005, Environmental health perspectives.

[36]  Pratim Biswas,et al.  Does nanoparticle activity depend upon size and crystal phase? , 2008, Nanotoxicology.

[37]  Karen Lowrie,et al.  Toxicity testing in the 21st century. , 2009, Risk analysis : an official publication of the Society for Risk Analysis.

[38]  Mariana F. Fernández,et al.  Human Exposure to Endocrine-Disrupting Chemicals and Prenatal Risk Factors for Cryptorchidism and Hypospadias: A Nested Case–Control Study , 2007, Environmental health perspectives.

[39]  W. M. Haynes.  CRC Handbook of Chemistry and Physics , 1990 .

[40]  C. Mirkin,et al.  Nanotechnology research directions for societal needs in 2020: summary of international study , 2011 .

[41]  John T Elliott,et al.  Stable nanoparticle aggregates/agglomerates of different sizes and the effect of their size on hemolytic cytotoxicity , 2011, Nanotoxicology.

[42]  W. Chan,et al.  Nanotoxicity: the growing need for in vivo study. , 2007, Current opinion in biotechnology.

[43]  Robert Rallo,et al.  Use of a high-throughput screening approach coupled with in vivo zebrafish embryo screening to develop hazard ranking for engineered nanomaterials. , 2011, ACS nano.

[44]  Jae H. Park,et al.  Drosophila caspases involved in developmentally regulated programmed cell death of peptidergic neurons during early metamorphosis , 2011, The Journal of comparative neurology.

[45]  Mark R. Wiesner,et al.  Preparation of Nanoparticle Dispersions from Powdered Material Using Ultrasonic Disruption , 2012 .

[46]  J. Bailar,et al.  Toxicity Testing in the 21st Century: A Vision and a Strategy , 2010, Journal of toxicology and environmental health. Part B, Critical reviews.

[47]  R. C. Weast.  CRC Handbook of Chemistry and Physics , 1973 .

[48]  Rose Amal,et al.  Biological impacts of TiO2 on human lung cell lines A549 and H1299: particle size distribution effects , 2011 .

[49]  Aravind Subramanian,et al.  Perturbational profiling of nanomaterial biologic activity , 2008, Proceedings of the National Academy of Sciences.

[50]  B. Derjaguin,et al.  Theory of the stability of strongly charged lyophobic sols and of the adhesion of strongly charged particles in solutions of electrolytes , 1993 .

[51]  Y. Lalatonne,et al.  Precipitation-redispersion of cerium oxide nanoparticles with poly(acrylic acid): toward stable dispersions. , 2005, Langmuir : the ACS journal of surfaces and colloids.

[52]  Marc Daigneault,et al.  The Identification of Markers of Macrophage Differentiation in PMA-Stimulated THP-1 Cells and Monocyte-Derived Macrophages , 2010, PloS one.