Ultraviolet-stimulated thymidine incorporation in xeroderma pigmentosum lymphocytes.

Abstract Autoradiographic and liquid scintillation counting techniques were utilized to measure the ultraviolet (UV)-stimulated incorporation of radioactive thymidine during repair replication of the UV-damaged deoxyribonucleic acid (DNA) of irradiated peripheral blood lymphocytes from 4 patients with xeroderma pigmentosum (XDP) and from normal donors. Lymphocytes from 3 of the 4 patients had a markedly decreased rate of incorporation soon after irradiation in comparison with the rate obtained with normal donors' lymphocytes. However, the duration of incorporation into these 3 patients' lymphocytes was prolonged, so that XDP lymphocytes appear eventually to incorporate as much total thymidine as normal lymphocytes. Lymphocytes from the fourth patient had a rate and duration of thymidine incorporation after irradiation similar to normal lymphocytes. This patient, therefore, may have a different genetic and biochemical defect from the other XDP patients.

[1]  J. Robbins,et al.  Rapid and sensitive assay for D.N.A. synthesis in leucocyte cultures. , 1970, Lancet.

[2]  W. Reed,et al.  Defect in DNA Synthesis in Skin of Patients with Xeroderma Pigmentosum Demonstrated in vivo , 1970, Science.

[3]  J. Robbins,et al.  Antigen-induced blastogenesis: the human cell determining the specificity of response in vitro. , 1970, Journal of immunology.

[4]  J. Cohen,et al.  Different inherited levels of DNA repair replication in xeroderma pigmentosum cell strains after exposure to ultraviolet irradiation. , 1970, Mutation research.

[5]  D. Bootsma,et al.  Repair of x-ray damage in DNA of cultivated cells from patients having xeroderma pigmentosum. , 1970, Mutation research.

[6]  J. Cleaver.  DNA damage and repair in light-sensitive human skin disease. , 1970, The Journal of investigative dermatology.

[7]  R. Setlow,et al.  Evidence that xeroderma pigmentosum cells do not perform the first step in the repair of ultraviolet damage to their DNA. , 1969, Proceedings of the National Academy of Sciences of the United States of America.

[8]  F. Legallais,et al.  Growth of ultraviolet-damaged herpesvirus in xeroderma pigemntosum. , 1969, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[9]  R. Kelly,et al.  Excision of Thymine Dimers and Other Mismatched Sequences by DNA Polymerase of Escherichia coli , 1969, Nature.

[10]  J. Cleaver,et al.  Xeroderma pigmentosum: a human disease in which an initial stage of DNA repair is defective. , 1969, Proceedings of the National Academy of Sciences of the United States of America.

[11]  J. Cleaver,et al.  Xeroderma pigmentosum. Clinical and laboratory investigation of its basic defect. , 1969, JAMA.

[12]  J. Cleaver,et al.  Repair replication, unscheduled DNA synthesis, and the repair of mammalian DNA. , 1969, Radiation research.

[13]  J. Cleaver.  Repair replication of mammalian cell DNA: effects of compounds that inhibit DNA synthesis or dark repair. , 1969, Radiation research.

[14]  C. Craddock,et al.  Deoxyribonucleic acid (DNA) repair replication of ultraviolet (UV) irradiated normal and leukemic leukocytes. , 1969, The Journal of laboratory and clinical medicine.

[15]  R. Evans,et al.  Unscheduled incorporation of thymidine in ultraviolet-irradiated human lymphocytes. , 1968, Radiation research.

[16]  J. Cleaver.  Defective Repair Replication of DNA in Xeroderma Pigmentosum , 1968, Nature.

[17]  Strauss Bs.  DNA repair mechanisms and their relation to mutation and recombination. , 1968 .

[18]  R. Setlow.  The photochemistry, photobiology, and repair of polynucleotides. , 1968, Progress in nucleic acid research and molecular biology.

[19]  L. Grossman,et al.  Enzymes involved in the early stages of repair of ultraviolet-irradiated DNA. , 1968, Cold Spring Harbor symposia on quantitative biology.

[20]  H. Lynch,et al.  Xeroderma pigmentosum, malignant melanoma, and congenital ichthyosis. A family study. , 1967, Archives of dermatology.

[21]  L. Tolmach,et al.  Responses of synchronous populations of HeLa cells to ultraviolet irradiation at selected stages of the generation cycle. , 1967, Radiation research.

[22]  L. Tolmach,et al.  Inhibition of DNA synthesis in HeLa cells by hydroxyurea. , 1967, Cancer research.

[23]  R. Painter,et al.  RADIATION-STIMULATED DNA SYNTHESIS IN CULTURED MAMMALIAN CELLS , 1966, The Journal of cell biology.

[24]  W. K. Sinclair.  Hydroxyurea: Differential Lethal Effects on Cultured Mammalian Cells during the Cell Cycle , 1965, Science.

[25]  W. Reed,et al.  XERODERMA PIGMENTOSUM WITH NEUROLOGICAL COMPLICATIONS: THE DE SANCTIS-CACCHIONE SYNDROME. , 1965 .

[26]  L. Penrose,et al.  Xeroderma pigmentosum‐its inheritance and relationship to the ABO blood‐group system , 1964, Annals of human genetics.

[27]  P. Hanawalt,et al.  EVIDENCE FOR REPAIR-REPLICATION OF ULTRAVIOLET DAMAGED DNA IN BACTERIA. , 1964, Journal of molecular biology.

[28]  P. Howard-Flanders,et al.  RELEASE OF ULTRAVIOLET LIGHT-INDUCED THYMINE DIMERS FROM DNA IN E. COLI K-12. , 1964, Proceedings of the National Academy of Sciences of the United States of America.

[29]  R. Setlow,et al.  THE DISAPPEARANCE OF THYMINE DIMERS FROM DNA: AN ERROR-CORRECTING MECHANISM. , 1964, Proceedings of the National Academy of Sciences of the United States of America.

[30]  E. Cronkite,et al.  Deoxyribonucleic acid synthesizing cells in peripheral blood of normal human beings. , 1958, Science.