Simultaneous determination of lactate and pyruvate in human sweat using reversed-phase high-performance liquid chromatography: a noninvasive approach.

The simultaneous determination of lactate and pyruvate in sweat has been performed using reversed phase high-performance liquid chromatography (RP-HPLC) with UV detection at 220 nm. The calibration curves were linear in the investigated range 0.3 - 350 mm of lactate, 0.003- 1 mm of pyruvate. The sensitivity was good with a limit of detection of 0.03 mm for lactate and 0.001 mm for pyruvate. Recoveries evaluated for the entire procedure were 102 ± 0.1 and 96 ± 0.1 for lactate and pyruvate, respectively. The method was successfully applied to analysis of sweat in 8 athletes at rest (pilocarpine sweating) and during physical exercise.

[1]  N. Liappis,et al.  Quantitative study of free amino acids in human eccrine sweat excreted from the forearms of healthy trained and untrained men during exercise , 2004, European Journal of Applied Physiology and Occupational Physiology.

[2]  Luc Léger,et al.  Methods to Determine Aerobic Endurance , 2002, Sports medicine.

[3]  F C Luft,et al.  Lactic acidosis update for critical care clinicians. , 2001, Journal of the American Society of Nephrology : JASN.

[4]  J. M. Green,et al.  Effects of High and Low Blood Lactate Concentrations on Sweat Lactate Response , 2000, International journal of sports medicine.

[5]  G. Mitchell,et al.  Diagnostic accuracy of blood lactate-to-pyruvate molar ratio in the differential diagnosis of congenital lactic acidosis. , 2007, Clinical chemistry.

[6]  L. Niskanen,et al.  Analytical investigation: assay of D-lactate in diabetic plasma and urine. , 2008, Clinical biochemistry.

[7]  Q. Dong,et al.  Direct electrochemical determination of pyruvate in human sweat by capillary zone electrophoresis , 2001, Electrophoresis.

[8]  K. Sahlin,et al.  Repetitive static muscle contractions in humans —a trigger of metabolic and oxidative stress? , 2004, European Journal of Applied Physiology and Occupational Physiology.

[9]  F W Sunderman,et al.  Ion-exchange chromatography of amino acids in sweat collected from healthy subjects during sauna bathing. , 1974, Clinical chemistry.

[10]  R. Thompson,et al.  Sweat lactate in man is derived from blood glucose. , 1971, Journal of applied physiology.

[11]  K. Sahlin,et al.  Regulation of lactic acid production during exercise. , 1988, Journal of applied physiology.

[12]  H. Ju,et al.  Simultaneous determination of l- and d-lactic acid in plasma by capillary electrophoresis. , 2005, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[13]  J. Henriksson,et al.  Redox state and lactate accumulation in human skeletal muscle during dynamic exercise. , 1987, The Biochemical journal.

[14]  O. Faude,et al.  Bicarbonate infusion and pH clamp moderately reduce hyperventilation during ramp exercise in humans. , 2007, Journal of applied physiology.

[15]  Gwang Lee,et al.  Simultaneous clinical monitoring of lactic acid, pyruvic acid and ketone bodies in plasma as methoxime/tert-butyldimethylsilyl derivatives by gas chromatography-mass spectrometry in selected ion monitoring mode. , 2008, Biomedical chromatography : BMC.

[16]  S. Ohmori,et al.  D-Lactate concentrations in blood, urine and sweat before and after exercise , 2005, European Journal of Applied Physiology and Occupational Physiology.

[17]  E. Newsholme,et al.  Biochemistry for the Medical Sciences , 1983 .

[18]  W. Neill,et al.  Effect of decreased O2 supply to tissue on the lactate: pyruvate ratio in blood. , 1969, The Journal of clinical investigation.

[19]  K. Naka,et al.  Lactate analyzer with continuous blood sampling for monitoring blood lactate during physical exercise. , 1991, Clinical chemistry.

[20]  D. Lin,et al.  A method for lactate and pyruvate determination in filter-paper dried blood spots. , 2009, Journal of chromatography. A.

[21]  L S Lamont,et al.  Sweat lactate secretion during exercise in relation to women's aerobic capacity. , 1987, Journal of applied physiology.

[22]  B. HADORN,et al.  Free Amino-acids in Human Sweat from Different Parts of the Body , 1967, Nature.

[23]  A. G. Tonevitsky,et al.  Relationship between Lactate Concentrations in Active Muscle Sweat and Whole Blood , 2010, Bulletin of Experimental Biology and Medicine.

[24]  K. Alberti,et al.  Continuous monitoring in vivo of blood glucose, lactate, alanine and 3-hydroxybutyrate. , 1978, Clinica chimica acta; international journal of clinical chemistry.

[25]  R. Lomax,et al.  Gender differences in sweat lactate , 2000, European Journal of Applied Physiology.

[26]  L. Forni,et al.  Elevation of anions in exercise-induced acidosis: a study by ion-exchange chromatography/mass spectrometry. , 2008, Biomedical chromatography : BMC.

[27]  H. Kanazawa,et al.  Enantiomeric determination of L- and D-lactic acid in human cerebrospinal fluid by chiral ligand exchange high-performance liquid chromatography. , 2000, Biomedical chromatography : BMC.

[28]  J. Coast,et al.  Effect of oxidative stress and acidosis on diaphragm contractile function. , 1997, The American journal of physiology.

[29]  K. Sato,et al.  Biology of sweat glands and their disorders. I. Normal sweat gland function. , 1989, Journal of the American Academy of Dermatology.

[30]  G. Zello,et al.  D-lactate in human and ruminant metabolism. , 2005, The Journal of nutrition.

[31]  G. Palleschi,et al.  Amperometric biosensor for determination of lactate in sweat , 1993 .

[32]  P. L. Altman,et al.  Blood and Other Body Fluids , 1961, Pediatrics.

[33]  L. Mcnaughton,et al.  Training to Enhance the Physiological Determinants of Long-Distance Running Performance , 2007, Sports medicine.

[34]  L. Cynober,et al.  Branched-chain keto-acids and pyruvate in blood: measurement by HPLC with fluorimetric detection and changes in older subjects. , 2000, Clinical chemistry.

[35]  C. Coltman,et al.  The amino acid content of sweat in normal adults. , 1966, The American journal of clinical nutrition.

[36]  D. Brocks,et al.  Metabolic acidosis: separation methods and biological relevance of organic acids and lactic acid enantiomers. , 2002, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[37]  G G Guilbault,et al.  Non-invasive biosensors in clinical analysis. , 1995, Biosensors & bioelectronics.

[38]  B. Saltin,et al.  Muscle and blood ammonia and lactate responses to prolonged exercise with hyperoxia. , 1987, Journal of applied physiology.

[39]  Isao Karube,et al.  Analysis of metabolites in sweat as a measure of physical condition , 1994 .

[40]  O. Faude,et al.  Lactate Threshold Concepts , 2009, Sports medicine.

[41]  T. Kuhara,et al.  Application of optical isomer analysis by diastereomer derivatization GC/MS to determine the condition of patients with short bowel syndrome. , 2006, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[42]  S. Thea,et al.  A simple, sensitive and efficient assay for the determination of D- and L-lactic acid enantiomers in human plasma by high-performance liquid chromatography. , 2011, Journal of chromatography. A.

[43]  J. Kulys,et al.  Amperometric methylene green-mediated pyruvate electrode based on pyruvate oxidase entrapped in carbon paste , 1992 .

[44]  Quantitative study of free amino acids in human eccrine sweat during normal conditions and exercise. , 1972, The American journal of clinical nutrition.

[45]  Michael J. Buono,et al.  The relationship between exercise intensity and the sweat lactate excretion rate , 2010, The Journal of Physiological Sciences.

[46]  Jilin Yan,et al.  An exercise degree monitoring biosensor based on electrochemiluminescent detection of lactate in sweat , 2010 .