Compact intraoperative imaging device for sentinel lymph node mapping

We have developed a novel real-time intraoperative fluorescence imaging device that can detect near-infrared (NIR) fluorescence and map sentinel lymph nodes (SLNs). In contrast to conventional imaging systems, this device is compact, portable, and battery-operated. It is also wearable and thus allows hands-free operation of clinicians. The system directly displays the fluorescence in its goggle eyepiece, eliminating the need for a remote monitor. Using this device in murine lymphatic mapping, the SLNs stained with indocyanine green (ICG) can be readily detected. Fluorescence-guided SLN resection under the new device was performed with ease. Ex vivo examination of resected tissues also revealed high fluorescence level in the SLNs. Histology further confirmed the lymphatic nature of the resected SLNs.

[1]  H. Cody,et al.  Isosulfan blue dye reactions during sentinel lymph node mapping for breast cancer. , 2002, Anesthesia and analgesia.

[2]  S. Achilefu,et al.  Novel receptor-targeted fluorescent contrast agents for in vivo tumor imaging. , 2000, Investigative radiology.

[3]  R. Simmons,et al.  A comparison of the adverse reactions associated with isosulfan blue versus methylene blue dye in sentinel lymph node biopsy for breast cancer. , 2005, American journal of surgery.

[4]  M. Morino,et al.  Sentinel lymph node biopsy in anal cancer: a review. , 2009, Gastroenterologie clinique et biologique.

[5]  Samuel Achilefu,et al.  Synthesis, in vitro receptor binding, and in vivo evaluation of fluorescein and carbocyanine peptide-based optical contrast agents. , 2002, Journal of medicinal chemistry.

[6]  L. Ngo,et al.  The FLARE™ Intraoperative Near-Infrared Fluorescence Imaging System: A First-in-Human Clinical Trial in Breast Cancer Sentinel Lymph Node Mapping , 2009, Annals of Surgical Oncology.

[7]  Janice K Popp,et al.  Health Problems Associated with International Business Travel , 2000, AAOHN journal : official journal of the American Association of Occupational Health Nurses.

[8]  David N. Krag,et al.  The sentinel node for staging breast cancer: Current review , 1999, Breast cancer.

[9]  John N Aarsvold,et al.  Update on detection of sentinel lymph nodes in patients with breast cancer. , 2005, Seminars in nuclear medicine.

[10]  Ali Ayhan,et al.  Lymphatic mapping and sentinel node biopsy in gynecological cancers: a critical review of the literature , 2008, World journal of surgical oncology.

[11]  Patrick Nicolas,et al.  Microvessel density as a prognostic factor in women with breast cancer: a systematic review of the literature and meta-analysis. , 2004, Cancer research.

[12]  Samuel Achilefu,et al.  Hands-free, wireless goggles for near-infrared fluorescence and real-time image-guided surgery. , 2011, Surgery.

[13]  Barbara A Pockaj,et al.  Injection of (99m)Tc-labeled sulfur colloid the day before operation for breast cancer sentinel lymph node mapping is as successful as injection the day of operation. , 2004, American journal of surgery.

[14]  S. Achilefu,et al.  Perspectives and potential applications of nanomedicine in breast and prostate cancer , 2013, Medicinal research reviews.

[15]  Franco Tagliaro,et al.  A current review , 2001 .

[16]  J. Mortensen,et al.  Positron emission tomography in the diagnosis and staging of lung cancer: a systematic, quantitative review. , 2001, The Lancet. Oncology.

[17]  T. Soussi,et al.  p53 Antibodies in the sera of patients with various types of cancer: a review. , 2000, Cancer research.

[18]  Robert C. Wolpert,et al.  A Review of the , 1985 .