RMA1, an Arabidopsis thaliana gene whose cDNA suppresses the yeast sec15 mutation, encodes a novel protein with a RING finger motif and a membrane anchor.

To identify molecules that function in the plant secretory pathway, we screened for Arabidopsis thaliana cDNA clones that complement the temperature-sensitive (ts), secretion-deficient sec15 mutation of yeast Saccharomyces cerevisiae. RMA1, one of the genes obtained in this screening, suppressed not only the ts growth of sec15 but also its secretory defect. RMA1 is not a structural homologue of SEC15 but encodes a novel 28 kDa protein with a RING finger motif and a C-terminal membrane-anchoring domain. Mutational analysis indicates that the RING finger motif of RMA1 is important for its suppression activity. In Arabidopsis plant, RMA1 is ubiquitously expressed. A search for homologous proteins in the database revealed that Arabidopsis, nematode, mouse and human possess close homologues of RMA1.

[1]  S. Emr,et al.  A novel RING finger protein complex essential for a late step in protein transport to the yeast vacuole. , 1997, Molecular biology of the cell.

[2]  M. Veenhuis,et al.  Peroxisomes: Organelles at the crossroads. , 1997, Trends in cell biology.

[3]  H. Kouchi,et al.  The AtVAM3 Encodes a Syntaxin-related Molecule Implicated in the Vacuolar Assembly in Arabidopsis thaliana * , 1997, The Journal of Biological Chemistry.

[4]  J. Zou,et al.  Cloning and molecular characterization of an Arabidopsis thaliana RING zinc finger gene expressed preferentially during seed development. , 1997, Gene.

[5]  R. Scheller,et al.  SNAREs and NSF in targeted membrane fusion. , 1997, Current opinion in cell biology.

[6]  P. Novick,et al.  Sec2p Mediates Nucleotide Exchange on Sec4p and Is Involved in Polarized Delivery of Post-Golgi Vesicles , 1997, The Journal of cell biology.

[7]  J. Moss,et al.  Interaction of the GTP-binding and GTPase-activating Domains of ARD1 Involves the Effector Region of the ADP-ribosylation Factor Domain* , 1997, The Journal of Biological Chemistry.

[8]  J. Avruch,et al.  An intact Raf zinc finger is required for optimal binding to processed Ras and for ras-dependent Raf activation in situ , 1997, Molecular and cellular biology.

[9]  S. Emr,et al.  A Novel RING Finger Protein, Vps8p, Functionally Interacts with the Small GTPase, Vps21p, to Facilitate Soluble Vacuolar Protein Localization* , 1996, The Journal of Biological Chemistry.

[10]  C. L. Jackson,et al.  Nucleotide exchange on ARF mediated by yeast Geal protein , 1996, Nature.

[11]  P. Novick,et al.  The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae. , 1996, The EMBO journal.

[12]  W. Xie,et al.  Two human cDNAs, including a homolog of Arabidopsis FUS6 (COP11), suppress G-protein- and mitogen-activated protein kinase-mediated signal transduction in yeast and mammalian cells , 1996, Molecular and cellular biology.

[13]  A. Nakano,et al.  An Arabidopsis gene isolated by a novel method for detecting genetic interaction in yeast encodes the GDP dissociation inhibitor of Ara4 GTPase. , 1996, The Plant cell.

[14]  H. Moser,et al.  A unified nomenclature for peroxisome biogenesis factors , 1996, The Journal of cell biology.

[15]  Josep Rizo,et al.  Synaptotagmins: C2-Domain Proteins That Regulate Membrane Traffic , 1996, Neuron.

[16]  K. Torii,et al.  Expression of an N-terminal fragment of COP1 confers a dominant-negative effect on light-regulated seedling development in Arabidopsis. , 1996, The Plant cell.

[17]  P. Freemont,et al.  Does this have a familiar RING? , 1996, Trends in biochemical sciences.

[18]  T. Okita,et al.  COMPARTMENTATION OF PROTEINS IN THE ENDOMEMBRANE SYSTEM OF PLANT CELLS. , 1996, Annual review of plant physiology and plant molecular biology.

[19]  J. H. Chou,et al.  Rab3 reversibly recruits rabphilin to synaptic vesicles by a mechanism analogous to raf recruitment by ras. , 1996, The EMBO journal.

[20]  W. Lukowitz,et al.  Cytokinesis in the Arabidopsis Embryo Involves the Syntaxin-Related KNOLLE Gene Product , 1996, Cell.

[21]  S. Yokoyama,et al.  Cysteine-rich Region of Raf-1 Interacts with Activator Domain of Post-translationally Modified Ha-Ras (*) , 1995, The Journal of Biological Chemistry.

[22]  A. Nakano,et al.  Membrane protein retrieval from the Golgi apparatus to the endoplasmic reticulum (ER): characterization of the RER1 gene product as a component involved in ER localization of Sec12p. , 1995, Molecular biology of the cell.

[23]  J. Berg,et al.  Formation of the peroxisome lumen is abolished by loss of Pichia pastoris Pas7p, a zinc-binding integral membrane protein of the peroxisome , 1995, Molecular and cellular biology.

[24]  N. Raikhel,et al.  An Arabidopsis syntaxin homologue isolated by functional complementation of a yeast pep12 mutant. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[25]  L. Schauser,et al.  PZF, a cDNA Isolated from Lotus japonicus and Soybean Root Nodule Libraries, Encodes a New Plant Member of the RING-Finger Family of Zinc-Binding Proteins , 1995, Plant physiology.

[26]  I. Herskowitz.  MAP kinase pathways in yeast: For mating and more , 1995, Cell.

[27]  J. Rothman,et al.  Mechanisms of intracellular protein transport , 1994, Nature.

[28]  Reinhard Jahn,et al.  Vesicle fusion from yeast to man , 1994, Nature.

[29]  C. Gillmor,et al.  EMB30 is essential for normal cell division, cell expansion, and cell adhesion in Arabidopsis and encodes a protein that has similarity to Sec7 , 1994, Cell.

[30]  S. Emr,et al.  The sorting receptor for yeast vacuolar carboxypeptidase Y is encoded by the VPS10 gene , 1994, Cell.

[31]  X. Hua,et al.  SREBP-1, a membrane-bound transcription factor released by sterol-regulated proteolysis , 1994, Cell.

[32]  H. Ronne,et al.  Yeast syntaxins Sso1p and Sso2p belong to a family of related membrane proteins that function in vesicular transport. , 1993, The EMBO journal.

[33]  R. Scheller,et al.  The molecular machinery for secretion is conserved from yeast to neurons. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[34]  C. d’Enfert,et al.  Fission yeast and a plant have functional homologues of the Sar1 and Sec12 proteins involved in ER to Golgi traffic in budding yeast. , 1992, The EMBO journal.

[35]  S. Emr,et al.  A putative zinc finger protein, Saccharomyces cerevisiae Vps18p, affects late Golgi functions required for vacuolar protein sorting and efficient alpha-factor prohormone maturation , 1991, Molecular and cellular biology.

[36]  K. Becherer,et al.  Isolation and characterization of PEP3, a gene required for vacuolar biogenesis in Saccharomyces cerevisiae , 1991, Molecular and cellular biology.

[37]  T. Berleth,et al.  Mutations affecting body organization in the Arabidopsis embryo , 1991, Nature.

[38]  R. Wright,et al.  Isolation and characterization of PEP5, a gene essential for vacuolar biogenesis in Saccharomyces cerevisiae. , 1990, Genetics.

[39]  M. Muramatsu,et al.  A novel GTP-binding protein, Sar1p, is involved in transport from the endoplasmic reticulum to the Golgi apparatus , 1989, The Journal of cell biology.

[40]  P. Novick,et al.  The Sec15 protein responds to the function of the GTP binding protein, Sec4, to control vesicular traffic in yeast , 1989, The Journal of cell biology.

[41]  R. Sikorski,et al.  A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. , 1989, Genetics.

[42]  J. Thorner,et al.  Yeast prohormone processing enzyme (KEX2 gene product) is a Ca2+-dependent serine protease. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[43]  R. Schekman,et al.  A membrane glycoprotein, Sec12p, required for protein transport from the endoplasmic reticulum to the Golgi apparatus in yeast , 1988, The Journal of cell biology.

[44]  K. Arai,et al.  GPA1, a haploid-specific essential gene, encodes a yeast homolog of mammalian G protein which may be involved in mating factor signal transduction , 1987, Cell.

[45]  P. Novick,et al.  A ras-like protein is required for a post-Golgi event in yeast secretion , 1987, Cell.

[46]  Andrew R. Cherenson,et al.  The structure of an antigenic determinant in a protein , 1984, Cell.

[47]  P. Seeburg,et al.  The primary structure of the Saccharomyces cerevisiae gene for 3-phosphoglycerate kinase. , 1982, Nucleic acids research.

[48]  R. Doolittle,et al.  A simple method for displaying the hydropathic character of a protein. , 1982, Journal of molecular biology.

[49]  F. Sanger,et al.  DNA sequencing with chain-terminating inhibitors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[50]  R. Palmiter.  Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid. , 1974, Biochemistry.

[51]  C. Creutz,et al.  Synaptotagmin II expression partially rescues the growth defect of the yeast sec15 secretory mutant , 1996, Biology of the cell.

[52]  G. Jürgens,et al.  Apical-basal pattern formation in the Arabidopsis embryo: studies on the role of the gnom gene , 1993 .

[53]  H. Uchimiya,et al.  Isolation and analysis of cDNAs encoding small GTP-binding proteins of Arabidopsis thaliana. , 1991, Gene.

[54]  K. Matsumoto,et al.  [Regulatory circuits for gene expression: the metabolism of galactose and phosphate in Saccharomyces cerevisiae]. , 1984, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme.