Ingestion of IH636 grape seed proanthocyanidin extract to prevent selenite‐induced oxidative stress in experimental cataract

PURPOSE: To investigate whether dietary supplementation with IH636 grape seed proanthocyanidin extract (GSPE) prevents selenite‐induced cataract. SETTING: Department of Ophthalmology, Gulhane Military Medical Academy, Ankara, Turkey. METHODS: Thirty Spraque‐Dawley rat litters were put randomly into 3 groups. In group 1 (n = 10), sodium selenite (30 nmol/g body weight) was injected subcutaneously on postpartum day 10. In group 2 (n = 10), sodium selenite (30 nmol/g body weight) was injected on postpartum day 10 and oral GSPE (100 mg/kg body weight) was given for 1 week after sodium selenite injection. Only subcutaneous saline was injected in group 3 (control, n = 10). The development of cataract was assessed for 3 weeks, and its density was graded and photographed with a slitlamp. Removed rat lenses were analyzed for glutathione (GSH) and malondialdehyde (MDA). RESULTS: All of the rats in group 1 had cataract between stage 6 and stage 3. In group 2, only 5 of 10 eyes had cataract between stage 3 and stage 2 and no cataract occurred in the remaining 5 rats. The difference between mean cataract stages in group 1 and group 2 was significant (P<.05). The mean GSH level in group 1 was significantly lower than in group 2 and controls (P<.05). The mean MDA level in group 1 was significantly higher than in group 2 and controls (P<.05). CONCLUSIONS: IH636 grape seed proanthocyanidin extract effectively suppressed cataract formation in rats. Routine consumption of grape seed proanthocyanidin extract in the form of food or dietary supplement may offer a prophylactic measure against onset and progression of cataract.

[1]  J. Harding,et al.  Free and protein-bound glutathione in normal and cataractous human lenses. , 1970, The Biochemical journal.

[2]  G. Ellman,et al.  Tissue sulfhydryl groups. , 1959, Archives of biochemistry and biophysics.

[3]  F. Giblin,et al.  Glutathione and lens epithelial function. , 1976, Investigative ophthalmology.

[4]  John I. Clark,et al.  Effect of selected anti-cataract agents on opacification in the selenite cataract model. , 1996, Experimental eye research.

[5]  S. Srivastava,et al.  Green Tea (Camellia sinensis) Protects against Selenite-Induced Oxidative Stress in Experimental Cataractogenesis , 2002, Ophthalmic Research.

[6]  A. Spector,et al.  Oxidative stress‐induced cataract: mechanism of action , 1995, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[7]  M. Kikuchi,et al.  Safety evaluation of proanthocyanidin-rich extract from grape seeds. , 2002, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[8]  H. Nishigori,et al.  An animal model for cataract research: cataract formation in developing chick embryo by glucocorticoid. , 1983, Experimental eye research.

[9]  W. Rathbun,et al.  Inhibition of Na,K-ATPase by sodium selenite and reversal by glutathione. , 1986, Current eye research.

[10]  C. Sen,et al.  Molecular mechanisms of cardioprotection by a novel grape seed proanthocyanidin extract. , 2003, Mutation research.

[11]  S. Srivastava,et al.  Role of glutathione in the prevention of cataractogenesis in rat lenses. , 1982, Current eye research.

[12]  K. Whitney,et al.  Subchronic 3-month oral toxicity study of grape seed and grape skin extracts. , 2002, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[13]  H. Gerster Antioxidant vitamins in cataract prevention , 1989, Zeitschrift fur Ernahrungswissenschaft.

[14]  D. Bagchi,et al.  Protective effects of grape seed proanthocyanidins and selected antioxidants against TPA-induced hepatic and brain lipid peroxidation and DNA fragmentation, and peritoneal macrophage activation in mice. , 1998, General pharmacology.

[15]  L Bravo,et al.  Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance. , 2009, Nutrition reviews.

[16]  Ivana Oštádalová,et al.  Cataract induced by administration of a single dose of sodium selenite to suckling rats , 1978, Experientia.

[17]  F. Ursini,et al.  Grape seed proanthocyanidins prevent plasma postprandial oxidative stress in humans. , 2002, Journal of agricultural and food chemistry.

[18]  J. I. Clark,et al.  Delay or inhibition of rat lens opacification using pantethine and WR-77913. , 1996, Experimental eye research.

[19]  J. L. Hess,et al.  Calcium efflux in rat lens: Na/Ca-exchange related to cataract induced by selenite. , 1992, Current eye research.

[20]  T. Shearer,et al.  State of sulfhydryl in selenite cataract. , 1984, Toxicology and applied pharmacology.

[21]  J. I. Clark,et al.  Inhibition of lens opacification during the early stages of cataract formation. , 1995, Investigative ophthalmology & visual science.

[22]  S. Joshi,et al.  Free radicals and grape seed proanthocyanidin extract: importance in human health and disease prevention. , 2000, Toxicology.

[23]  S. Varma,et al.  Attenuation of sugar cataract by ethyl pyruvate , 1999, Molecular and Cellular Biochemistry.

[24]  K. Yagi,et al.  Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. , 1979, Analytical biochemistry.

[25]  Sujata Joshi,et al.  Lycopene attenuates oxidative stress induced experimental cataract development: an in vitro and in vivo study. , 2003, Nutrition.

[26]  M. Natsume,et al.  Ingestion of Proanthocyanidins Derived from Cacao Inhibits Diabetes-Induced Cataract Formation in Rats , 2004, Experimental biology and medicine.

[27]  T. Shearer,et al.  Selenite nuclear cataract: review of the model. , 1997, Molecular vision.