MAGE Xp-2: a member of the MAGE gene family isolated from an expression library using systemic lupus erythematosus sera.

Two regions of the genome contain members of the MAGE gene family; Xq27-qter and Xp21.3. We isolated a transcript, MAGE Xp-2, by screening a cDNA library from the human epithelial carcinoma cell line, HEp-2, using autoantibodies from patients with systemic lupus erythematosus (SLE). The open reading frame (ORF) of MAGE Xp-2 is entirely contained in exon 4, a signature feature of the MAGE gene family. While MAGE Xp-2 shares genomic homology with MAGE Xp-1, the predicted proteins are quite divergent. Specific primers were designed to reliably distinguish between MAGE Xp-1 and MAGE Xp-2 expression. MAGE Xp-2 is expressed in testis, but not in other normal tissues. It is also expressed strongly in two of seven melanoma cell lines and one of four breast carcinomas. MAGE gene expression may be important not only for tumor recognition and cancer therapy, but, because it is the apparent target of autoantibodies in SLE sera, it may also play a role in autoimmune diseases.

[1]  M. Hirohata,et al.  Identification of MAGE-1 and MAGE-4 proteins in spermatogonia and primary spermatocytes of testis. , 1995, Cancer research.

[2]  P. Chomez,et al.  Human gene MAGE-1, which codes for a tumor-rejection antigen, is expressed by some breast tumors. , 1992, International journal of cancer.

[3]  S. Shichijo,et al.  Induction of MAGE Genes in Lymphoid Cells by the Demethylating Agent 5‐Aza‐2′‐deoxycytidine , 1996, Japanese journal of cancer research : Gann.

[4]  F. Brasseur,et al.  Expression of mage genes by non‐small‐cell lung carcinomas , 1994, International journal of cancer.

[5]  P. Chomczyński,et al.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. , 1987, Analytical biochemistry.

[6]  H. Cedar,et al.  Role of DNA methylation in the regulation of transcription. , 1994, Current opinion in genetics & development.

[7]  Catia,et al.  A nonapeptide encoded by human gene MAGE-1 is recognized on HLA-A1 by cytolytic T lymphocytes directed against tumor antigen MZ2-E , 1992, The Journal of experimental medicine.

[8]  J. Becker,et al.  A member of the melanoma antigen‐encoding gene (MAGE) family is expressed in human skin during wound healing , 1994, International journal of cancer.

[9]  P. Chomez,et al.  A gene encoding an antigen recognized by cytolytic T lymphocytes on a human melanoma. , 1991, Science.

[10]  O. de Backer,et al.  The activation of human gene MAGE-1 in tumor cells is correlated with genome-wide demethylation. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[11]  D. Alarcón-Segovia,et al.  Autoantibodies in systemic lupus erythematosus. , 1996, Current opinion in rheumatology.

[12]  A. Monaco,et al.  Isolation and characterization of a MAGE gene family in the Xp21.3 region. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[13]  J. Strominger,et al.  Selective binding of self peptides to disease-associated major histocompatibility complex (MHC) molecules: a mechanism for MHC-linked susceptibility to human autoimmune diseases , 1995, The Journal of experimental medicine.

[14]  Adrian Bird,et al.  The essentials of DNA methylation , 1992, Cell.

[15]  L. Butterfield,et al.  Cloning and analysis of MART-1/Melan-A human melanoma antigen promoter regions. , 1997, Gene.

[16]  J. Ozols,et al.  Induction of antigen-specific cytolytic T cells in situ in human melanoma by immunization with synthetic peptide-pulsed autologous antigen presenting cells. , 1995, Proceedings of the National Academy of Sciences of the United States of America.