Quantitative trait loci for Fusarium head blight resistance in barley detected in a two-rowed by six-rowed population

Fusarium head blight (FHB) is a disease problem (primarily caused by Fusarium graminearum Schwabe) that affects the quality and yield of barley (Hordeum vulgare L.) grain. The objectives of this study were to identify the location of quantitative trait loci (QTL) for resistance to FHB in a two-rowed by six-rowed population, to examine the association of FHB resistance with heading date and the Vrsl (two-rowed spike morphology) locus, to validate the location of the major FHB resistance QTL primarily detected in the field, and to identify simple sequence repeat (SSR) markers linked to the QTL for FHB resistance. We created a genetic map of 143 molcular markers from a population derived from the parents Fredrickson (two-rowed, moderately resistant) and Stander (six-rowed, susceptible). The Fredrickson/Stander population was evaluated in two field environments by a grain spawn inoculation method, two field environments by a spray inoculation, and two greenhouse environments. QTL analysis detected three distinct regions on chromosome 2(2H) associated with FHB resistance; two of these regions were also associated with resistance to deoxynivalenol (DON) accumulation. One FHB resistance QTL was also associated with heading date, while another FHB resistance QTL was found associated with the Vrs1 locus. The third FHB resistance QTL was detected only in the greenhouse, but was coincident with a QTL for resistance to DON accumulation in the field. All three QTL were detected in the greenhouse, indicating that this environment may be useful for selecting FHB resistant barley genotypes. SSR markers were identified that are linked with each QTL and could prove useful for marker-assisted trait manipulation. Finally, the two major QTL detected in the field were validated using an independent population with Fredrickson and Stander parents.

[1]  M. Maroof,et al.  Development of simple sequence repeat DNA markers and their integration into a barley linkage map , 1996, Theoretical and Applied Genetics.

[2]  D. Kudrna,et al.  A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome , 1993, Theoretical and Applied Genetics.

[3]  A. Graner,et al.  Construction of an RFLP map of barley , 1991, Theoretical and Applied Genetics.

[4]  J. Jensen,et al.  Quantitative trait loci for grain yield and yield components in a cross between a six-rowed and a two-rowed barley , 2004, Euphytica.

[5]  S. Ullrich,et al.  QTL analysis of agronomic traits in barley based on the doubled haploid progeny of two elite North American varieties representing different germplasm groups , 2001, Theoretical and Applied Genetics.

[6]  D. Kudrna,et al.  Molecular mapping of the Oregon Wolfe Barleys: a phenotypically polymorphic doubled-haploid population , 2001, Theoretical and Applied Genetics.

[7]  P. Cregan,et al.  DNA markers for Fusarium head blight resistance QTLs in two wheat populations , 2001, Theoretical and Applied Genetics.

[8]  J. Rudd,et al.  Host Plant Resistance Genes for Fusarium Head Blight: Sources, Mechanisms, and Utility in Conventional Breeding Systems , 2001 .

[9]  M. Morgante,et al.  A simple sequence repeat-based linkage map of barley. , 2000, Genetics.

[10]  C. K. Evans,et al.  Biosynthesis of Deoxynivalenol in Spikelets of Barley Inoculated with Macroconidia of Fusarium graminearum. , 2000, Plant disease.

[11]  L. Prom,et al.  Mapping of quantitative trait Loci for fusarium head blight resistance in barley. , 2000, Phytopathology.

[12]  C. Windels.  Economic and social impacts of fusarium head blight: changing farms and rural communities in the northern great plains. , 2000, Phytopathology.

[13]  Kevin P. Smith,et al.  Quantitative trait loci associated with resistance to Fusarium head blight and kernel discoloration in barley , 1999, Theoretical and Applied Genetics.

[14]  Blair L. Waldron,et al.  RFLP mapping of QTL for fusarium head blight resistance in wheat , 1999 .

[15]  G. Bai,et al.  Amplified fragment length polymorphism markers linked to a major quantitative trait locus controlling scab resistance in wheat. , 1999, Phytopathology.

[16]  J. Anderson,et al.  RFLP Markers Associated with High Grain Protein from Triticum turgidum L. var. dicoccoides Introgressed into Hard Red Spring Wheat , 1999 .

[17]  G. Scoles,et al.  Verifications of a Quantitative Trait Locus Affecting Agronomic Traits in Two-Row Barley , 1999 .

[18]  D. Kudrna,et al.  Does function follow form? Principal QTLs for Fusarium head blight (FHB) resistance are coincident with QTLs for inflorescence traits and plant height in a doubled-haploid population of barley , 1999, Theoretical and Applied Genetics.

[19]  H. Jeleń,et al.  Analysis of Deoxynivalenol and Its Derivatives (Batch and Single Kernel) Using Gas Chromatography/Mass Spectrometry , 1998 .

[20]  Roger Jones,et al.  Scab of Wheat and Barley: A Re-emerging Disease of Devastating Impact. , 1997, Plant disease.

[21]  T. Choo,et al.  Genetic analysis of a two-row × six-row cross of barley using doubled-haploid lines , 1997, Theoretical and Applied Genetics.

[22]  P. Lindhout,et al.  Comparison and integration of four barley genetic maps. , 1996, Genome.

[23]  B J Bassam,et al.  Fast and sensitive silver staining of DNA in polyacrylamide gels. , 1991, Analytical biochemistry.

[24]  B. Gill,et al.  A genetic linkage map of Triticum tauschii (DD) and its relationship to the D genome of bread wheat (AABBDD) , 1991 .

[25]  S. Tanksley,et al.  Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) , 1991 .

[26]  K. Takeda.  Selection Response and Parent-offspring Correlation of the Resistance to Fusarium Head Blight in Barley , 1990 .

[27]  K. Takeda,et al.  Establishing the Testing Method and a Search for the Resistant Varieties to Fusarium Head Blight in Barley , 1989 .

[28]  K. A. Gomez,et al.  Statistical Procedures for Agricultural Research. , 1984 .