Spectrophotometric analysis of polysaccharide/milk protein interactions with methylene blue using Independent Components Analysis

Abstract Interactions between xanthan gum, carrageenan, guar gum and milk protein were studied under various conditions of ionic strengths, temperature and pH. The proposed methodology was to examine the associative interactions by using a methylene blue (MB) spectrophotometric method combined with data analysis by a chemometric method. Independent Components Analysis (ICA) simplified the interpretation of the spectrophotometric results by decomposing absorbance spectral data into “pure signals” that could be related to chemical compounds. Addition of milk protein to MB/xanthan gum or carrageenan solution gave rise to spectral changes indicating electrostatic interactions between positively charged regions of milk protein and anionic polysaccharides at neutral pH and low ionic strength. Thus, it has been shown that negative polysaccharides are able to interact with milk proteins only in absence of NaCl. On the other hand, it was shown that no attractive interactions were established in neutral guar gum/milk protein systems, which highlighted the contribution of the charge density to the interactions. Through acidification, associative interactions between xanthan gum and milk proteins were strongly enhanced as shown by changes in the IC proportions. Therefore, ICA proved to be an efficient tool to facilitate interpretation of spectrophotometric data and identify associative interactions.

[1]  C. Sánchez,et al.  Protein–polysaccharide interactions , 2000 .

[2]  R. Moore,et al.  THE CONFORMATION OF HYALURONIC ACID AND CHONDROITIN SULFATE C: THE METACHROMATIC REACTION. , 1964, Biochimica et biophysica acta.

[3]  R. Queirós,et al.  On the "concentration-driven" methylene blue dimerization. , 2009, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.

[4]  Barbara Katzbauer,et al.  Properties and applications of xanthan gum , 1998 .

[5]  Hongbin Zhang,et al.  The stabilization mechanism of acidified milk drinks induced by carboxymethylcellulose , 2007 .

[6]  D. Jouan-Rimbaud Bouveresse,et al.  Two novel methods for the determination of the number of components in independent components analysis models , 2012 .

[7]  F. Weinbreck,et al.  Complex coacervation of proteins and anionic polysaccharides , 2004 .

[8]  V. Kiosseoglou,et al.  Whey protein-carboxymethylcellulose interaction in solution and in oil-in-water emulsion systems. Effect on emulsion stability , 2009 .

[9]  C. Rolin,et al.  Electrosorption of pectin onto casein micelles. , 2002, Biomacromolecules.

[10]  James V. Stone.  Independent Component Analysis: A Tutorial Introduction , 2007 .

[11]  D. Jouan-Rimbaud Bouveresse,et al.  Independent components analysis with the JADE algorithm , 2012 .

[12]  Douglas N. Rutledge,et al.  Durbin–Watson statistic as a morphological estimator of information content , 2002 .

[13]  T. Kobori,et al.  pH-Dependent interaction between sodium caseinate and xanthan gum , 2009 .

[14]  Erkki Oja,et al.  Independent component analysis: algorithms and applications , 2000, Neural Networks.

[15]  C. Michon,et al.  Iota-carrageenan/casein micelles interactions: evidence at different scales , 2003 .

[16]  G. Cuvelier,et al.  Gelatin/carrageenan interactions in coil and ordered conformations followed by a methylene blue spectrophotometric method , 2002 .

[17]  N. Garti,et al.  Formation and characterization of amphiphilic conjugates of whey protein isolate (WPI)/xanthan to improve surface activity , 2007 .

[18]  Adrián A. Perez,et al.  Interactions between milk whey protein and polysaccharide in solution , 2009 .

[19]  C. Michon,et al.  Gelatin/iota-carrageenan interactions in non-gelling conditions , 2000 .

[20]  C. Pan,et al.  Characterisation of interactions between fish gelatin and gum arabic in aqueous solutions. , 2012, Food chemistry.

[21]  Harjinder Singh,et al.  Viscosity, microstructure and phase behavior of aqueous mixtures of commercial milk protein products and xanthan gum , 2001 .

[22]  C. G. D. Kruif,et al.  Polysaccharide protein interactions , 2001 .

[23]  H. Klostermeyer,et al.  Polymer Science Concepts in Dairy Systems—an Overview of Milk Protein and Food Hydrocolloid Interaction , 1998 .

[24]  Peter Schurtenberger,et al.  Spinodal decomposition in a food colloid–biopolymer mixture: evidence for a linear regime , 2006, Journal of physics. Condensed matter : an Institute of Physics journal.

[25]  S. Turgeon,et al.  Protein/polysaccharide complexes and coacervates in food systems. , 2011, Advances in colloid and interface science.

[26]  C. G. D. Kruif,et al.  Interaction of pectin and casein micelles. , 2000 .

[27]  M. Corredig,et al.  Polysaccharide–protein interactions in dairy matrices, control and design of structures , 2011 .

[28]  R. Tuinier,et al.  The effect of depolymerised guar gum on the stability of skim milk , 2000 .

[29]  P. Nuss,et al.  Equilibrium and non-equilibrium complexes between bovine serum albumin and dextran sulfate—III. Methylene blue binding by equilibrium complexes , 1988 .

[30]  V. Tolstoguzov.  Some physico-chemical aspects of protein processing in foods. Multicomponent gels* , 1995 .

[31]  J. Doublier,et al.  Phase separation, rheology and microstructure of micellar casein–guar gum mixtures , 1999 .