A mathematical model for prediction of plasmid copy number and genetic stability in Escherichia coli

The design of bioreactors for genetically modified bacterial cultures would benefit from predictive models. Of particular importance is the interaction of the external environment, cell physiology, and control of plasmid copy number. We have recently developed a model based on the molecular mechanisms for control of replication of Co1E1 type plasmids. The inclusion of the plasmid model into a single‐cell E. coli model allows the explicit prediction of the interaction of cell physiology and plasmid‐encoded functions. The model predictions of the copy number of plasmids with the Co1E1 origin of replication carrying a variety of regulatory mutations is very close to that observed experimentally.

[1]  J E Bailey,et al.  Simulations of host–plasmid interactions in Escherichia coli: Copy number, promoter strength, and ribosome binding site strength effects on metabolic activity and plasmid gene expression , 1987, Biotechnology and bioengineering.

[2]  M. Ataai,et al.  Mathematical model for the control of ColE1 type plasmid replication. , 1986, Plasmid.

[3]  J. Bailey,et al.  Studies of Host‐Plasmid Interactions in Recombinant Microorganisms a , 1986, Annals of the New York Academy of Sciences.

[4]  M M Ataai,et al.  Simulation of CFSTR through development of a mathematical model for anaerobic growth of Escherichia coli cell population , 1985, Biotechnology and bioengineering.

[5]  M M Ataai,et al.  Simulation of the growth pattern of a single cell of Escherichia coli under anaerobic conditions , 1985, Biotechnology and bioengineering.

[6]  S. Aiba,et al.  Effects of temperature and dilution rate on the copy number of recombinant plasmid in continuous culture of Bacillus stearothermophilus (pLP11) , 1985, Biotechnology and bioengineering.

[7]  M L Shuler,et al.  Continuous Immobilized Recombinant Protein Production from E. coli Capable of Selective Protein Excretion: A Feasibility Study , 1985, Biotechnology progress.

[8]  J. Tomizawa.  Control of cole1 plasmid replication: Initial interaction of RNA I and the primer transcript is reversible , 1985, Cell.

[9]  J E Bailey,et al.  A segregated model for plasmid content and product synthesis in unstable binary fission recombinant organisms , 1985, Biotechnology and bioengineering.

[10]  R. Siegel,et al.  Kinetic study of instability of recombinant plasmid pPLc23trpAl in E. coli using two‐stage continuous culture system , 1985, Biotechnology and bioengineering.

[11]  J. Tomizawa.  Control of cole 1 plasmid replication: The process of binding of RNA I to the primer transcript , 1984, Cell.

[12]  J. Tomizawa,et al.  Control of cole 1 plasmid replication: Enhancement of binding of RNA I to the primer transcript by the rom protein , 1984, Cell.

[13]  S. Aiba,et al.  Effects of temperature on plasmid stability and penicillinase productivity of a transformant of Bacillus stearothermophilus. , 1984, Annals of the New York Academy of Sciences.

[14]  M M Domach,et al.  A finite representation model for an asynchronous culture of E. coli , 1984, Biotechnology and bioengineering.

[15]  L. Castagnoli,et al.  Control of pMB1 replication: inhibition of primer formation by Rop requires RNA1. , 1984, The EMBO journal.

[16]  R. Rodriguez,et al.  A Bacteriophage λ Cohesive Ends (cos) DNA Fragment Enhances the Fitness of Plasmid-Containing Bacteria Growing in Energy-Limited Chemostats , 1984, Bio/Technology.

[17]  M M Domach,et al.  Computer model for glucose‐limited growth of a single cell of Escherichia coli B/r‐A , 1984, Biotechnology and bioengineering.

[18]  James E. Bailey,et al.  A mathematical model for λdv plasmid replication: Analysis of wild-type plasmid , 1984 .

[19]  H. Masukata,et al.  Effects of point mutations on formation and structure of the RNA primer for ColE1 DNA replication , 1984, Cell.

[20]  J E Bailey,et al.  Analysis of growth rate effects on productivity of recombinant Escherichia coli populations using molecular mechanism models , 1984, Biotechnology and bioengineering.

[21]  D. Hartl,et al.  Transposable element IS50 improves growth rate of E. coli cells without transposition , 1983, Cell.

[22]  P. Gustafsson,et al.  The use of a partition locus to increase stability of tryptophan-operon-bearing plasmids in Escherichia coli. , 1983, Gene.

[23]  D. Moser,et al.  Characterization and complementation of pMB1 copy number mutant: effect of RNA I gene dosage on plasmid copy number and incompatibility , 1983, Journal of bacteriology.

[24]  T. Itoh,et al.  The origin of replication of plasmid p15A and comparative studies on the nucleotide sequences around the origin of related plasmids , 1983, Cell.

[25]  Michael L. Shuler,et al.  Mathematical Models of the Growth of Individual Cells: Tools for Testing Biochemical Mechanisms , 1983 .

[26]  J. Tomizawa,et al.  Specific cleavage of the p15A primer precursor by ribonuclease H at the origin of DNA replication. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[27]  D. Ollis,et al.  Batch fermentation kinetics with (unstable) recombinant cultures. , 1982, Biotechnology and bioengineering.

[28]  G. Cesareni,et al.  Control of ColE1 DNA replication: the rop gene product negatively affects transcription from the replication primer promoter. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[29]  T. Itoh,et al.  Plasmid ColE1 incompatibility determined by interaction of RNA I with primer transcript. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[30]  T. Imanaka,et al.  A PERSPECTIVE ON THE APPLICATION OF GENETIC ENGINEERING: STABILITY OF RECOMBINANT PLASMID , 1981, Annals of the New York Academy of Sciences.

[31]  J. Adams,et al.  The maintenance of Plasmid-containing organisms in populations of Escherichia coli. , 1981, Journal of general microbiology.

[32]  T. Itoh,et al.  Inhibition of ColE1 RNA primer formation by a plasmid-specified small RNA. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[33]  S. Molin,et al.  Partitioning of plasmid R1 in Escherichia coli. I. Kinetics of loss of plasmid derivatives deleted of the par region. , 1980, Plasmid.

[34]  Stanley N Cohen,et al.  Partitioning of bacterial plasmids during cell division: a cis-acting locus that accomplishes stable plasmid inheritance , 1980, Cell.

[35]  D. Sherratt,et al.  Trans-complementable copy-number mutants of plasmid ColE1 , 1980, Nature.

[36]  S. Conrad,et al.  Role of plasmid-coded RNA and ribonuclease III in plasmid DNA replication , 1979, Cell.

[37]  J. H. Slater,et al.  The influence of the growth environment on the stability of a drug resistance plasmid in Escherichia coli K12. , 1979, Journal of general microbiology.

[38]  J. Inselburg.  ColE1 plasmid mutants affecting growth of an Escherichia coli recB recC sbcB mutant , 1978, Journal of bacteriology.

[39]  Jeffrey H. Miller.  Experiments in molecular genetics , 1972 .