Estimation of intracellular pH in argon laser scanning confocal microscopes with a laser line of 488 nm

The reliable and widely used pH-sensitive indicator BCECF (2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein) can also be employed in a classical confocal laser scanning microscopes using 488 nm argon laser excitation line. The intracellular pH maps based on the fluorescence emission ratio tally with those obtained by other techniques.