High-throughput confinement and detection of single DNA molecules in aqueous microdroplets.

A droplet-based microfluidic system combined with high-sensitivity optical detection is used as a tool for high-throughput confinement and detection of single DNA molecules.

[1]  Daniel T Chiu,et al.  Sizing subcellular organelles and nanoparticles confined within aqueous droplets. , 2008, Analytical chemistry.

[2]  Monpichar Srisa-Art,et al.  High-throughput DNA droplet assays using picoliter reactor volumes. , 2007, Analytical chemistry.

[3]  D. Weitz,et al.  Electric control of droplets in microfluidic devices. , 2006, Angewandte Chemie.

[4]  Dan S. Tawfik,et al.  High-throughput screening of enzyme libraries: thiolactonases evolved by fluorescence-activated sorting of single cells in emulsion compartments. , 2005, Chemistry & biology.

[5]  Andreas Manz,et al.  Single-molecule fluorescence detection in microfluidic channels—the Holy Grail in μTAS? , 2005, Analytical and bioanalytical chemistry.

[6]  Abraham P. Lee,et al.  Microfluidic sorting of droplets by size , 2008 .

[7]  J. Michael Ramsey,et al.  Single-molecule detection limits in levitated microdroplets , 1991 .

[8]  Nigel Beard,et al.  Dealing with real samples: sample pre-treatment in microfluidic systems. , 2003, Lab on a chip.

[9]  Deirdre R. Meldrum,et al.  Life-on-a-chip , 2003, Nature Reviews Microbiology.

[10]  J. Michael Ramsey,et al.  Detection of single Rhodamine 6G molecules in levitated microdroplets , 1993 .

[11]  Monpichar Srisa-Art,et al.  Microdroplets: a sea of applications? , 2008, Lab on a chip.

[12]  Daniel Bratton,et al.  Development of quantitative cell-based enzyme assays in microdroplets. , 2008, Analytical chemistry.

[13]  Tza-Huei Wang,et al.  Cylindrical illumination confocal spectroscopy: rectifying the limitations of confocal single molecule spectroscopy through one-dimensional beam shaping. , 2008, Biophysical journal.

[14]  Dan Bratton,et al.  Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays. , 2009, Lab on a chip.