Multilocus sequence analysis of Porphyromonas gingivalis indicates frequent recombination.

In this study, the genetic relationship of 19 Porphyromonas gingivalis isolates from patients with periodontitis was investigated by multilocus sequence analysis. Internal 400-600 bp DNA fragments of the 10 chromosomal genes ef-tu, ftsQ, hagB, gpdxJ, pepO, mcmA, dnaK, recA, pga and nah were amplified by PCR and sequenced. No two isolates were identical at all 10 loci. Phylogenetic analyses indicated a panmictic population structure of P. gingivalis. Split decomposition analysis, calculation of homoplasy ratios and analyses of clustered polymorphisms all indicate that recombination plays a major role in creating the genetic heterogeneity of P. gingivalis. A standardized index of association of 0.0898 indicates that the P. gingivalis genes analysed are close to linkage equilibrium.

[1]  M. Stephens,et al.  Traces of Human Migrations in Helicobacter pylori Populations , 2003, Science.

[2]  S. Hamada,et al.  Functional Differences among FimA Variants of Porphyromonas gingivalis and Their Effects on Adhesion to and Invasion of Human Epithelial Cells , 2002, Infection and Immunity.

[3]  Mark Achtman,et al.  A Phylogenetic Perspective on Molecular Epidemiology , 2002 .

[4]  D. Falush,et al.  Recombination and mutation during long-term gastric colonization by Helicobacter pylori: Estimates of clock rates, recombination size, and minimal age , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[5]  M. Kilian,et al.  Evidence of Recombination in Porphyromonas gingivalis and Random Distribution of Putative Virulence Markers , 2001, Infection and Immunity.

[6]  S. Suerbaum,et al.  Allelic Diversity and Recombination inCampylobacter jejuni , 2001, Journal of bacteriology.

[7]  S. Hamada,et al.  Prevalence of Specific Genotypes of Porphyromonas gingivalis fimA and Periodontal Health Status , 2000, Journal of dental research.

[8]  S. Suerbaum.  Genetic variability within Helicobacter pylori. , 2000, International journal of medical microbiology : IJMM.

[9]  D. Roopenian,et al.  Heterogeneity of Porphyromonas gingivalis strains in the induction of alveolar bone loss in mice. , 2000, Oral microbiology and immunology.

[10]  H. Karch,et al.  Heterogeneity of the prtC gene of Porphyromonas gingivalis. , 2000, Oral microbiology and immunology.

[11]  I. Olsen,et al.  Genetic diversity of Porphyromonas gingivalis and its possible importance to pathogenicity , 2000, Acta odontologica Scandinavica.

[12]  T. Takehara,et al.  Sequencing, expression and biochemical characterization of the Porphyromonas gingivalis pepO gene encoding a protein homologous to human endothelin‐converting enzyme , 1999, FEBS letters.

[13]  S. Hamada,et al.  Distribution of Porphyromonas gingivalisStrains with fimA Genotypes in Periodontitis Patients , 1999, Journal of Clinical Microbiology.

[14]  M. Achtman,et al.  Evolution of Helicobacter pylori: the role of recombination. , 1999, Trends in microbiology.

[15]  T. Koga,et al.  A novel dnaK operon from Porphyromonas gingivalis 1 , 1999, FEBS letters.

[16]  S. Hanley,et al.  A 55-Kilodalton Immunodominant Antigen ofPorphyromonas gingivalis W50 Has Arisen via Horizontal Gene Transfer , 1999, Infection and Immunity.

[17]  Julio Rozas,et al.  DnaSP version 3: an integrated program for molecular population genetics and molecular evolution analysis , 1999, Bioinform..

[18]  T. A. Hall,et al.  BIOEDIT: A USER-FRIENDLY BIOLOGICAL SEQUENCE ALIGNMENT EDITOR AND ANALYSIS PROGRAM FOR WINDOWS 95/98/ NT , 1999 .

[19]  A. Kingman,et al.  Gingival recession, gingival bleeding, and dental calculus in adults 30 years of age and older in the United States, 1988-1994. , 1999, Journal of periodontology.

[20]  R. Hudson,et al.  Detecting linkage disequilibrium in bacterial populations. , 1998, Genetics.

[21]  R. Lamont,et al.  Life Below the Gum Line: Pathogenic Mechanisms ofPorphyromonas gingivalis , 1998, Microbiology and Molecular Biology Reviews.

[22]  J. M. Smith,et al.  Free recombination within Helicobacter pylori. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[23]  J. M. Smith,et al.  Detecting recombination from gene trees. , 1998, Molecular biology and evolution.

[24]  M. Achtman,et al.  Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[25]  Daniel H. Huson,et al.  SplitsTree: analyzing and visualizing evolutionary data , 1998, Bioinform..

[26]  H. Tobi,et al.  Predictors of tooth loss over 10 years in adult and elderly Chinese. , 1997, Community dentistry and oral epidemiology.

[27]  H. Fletcher,et al.  Nucleotide sequence of the Porphyromonas gingivalis W83 recA homolog and construction of a recA-deficient mutant , 1997, Infection and immunity.

[28]  A. Sheiham.  Is the chemical prevention of gingivitis necessary to prevent severe periodontitis? , 1997, Periodontology 2000.

[29]  V. Baelum,et al.  Predictors of destructive periodontal disease incidence and progression in adult and elderly Chinese. , 1997, Community dentistry and oral epidemiology.

[30]  H. Karch,et al.  Serodiagnosis of Porphyromonas gingivalis infection by immunoblot analysis with recombinant collagenase , 1996, Journal of clinical microbiology.

[31]  S. Dashper,et al.  Cloning, expression and sequence analysis of the genes encoding the heterodimeric methylmalonyl-CoA mutase of Porphyromonas gingivalis W50. , 1995, Gene.

[32]  A. Progulske-Fox,et al.  The cloning, expression and sequence analysis of a second Porphyromonas gingivalis gene that codes for a protein involved in hemagglutination. , 1995, Oral microbiology and immunology.

[33]  K. Nagai,et al.  A murC gene in Porphyromonas gingivalis 381. , 1995, Microbiology.

[34]  H. Kuramitsu,et al.  Proteases and Collagenases of Porphyromonas Gingivalis , 1995, Advances in dental research.

[35]  C. Cutler,et al.  Pathogenic strategies of the oral anaerobe, Porphyromonas gingivalis. , 1995, Trends in microbiology.

[36]  J. Thompson,et al.  CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. , 1994, Nucleic acids research.

[37]  Sudhir Kumar,et al.  MEGA: Molecular Evolutionary Genetics Analysis software for microcomputers , 1994, Comput. Appl. Biosci..

[38]  J. Slots,et al.  Clonal analysis of Porphyromonas gingivalis by the arbitrarily primed polymerase chain reaction. , 1994, Oral microbiology and immunology.

[39]  P. Gosselin,et al.  Polymerase chain reaction using arbitrary primer for the design and construction of a DNA probe specific for Porphyromonas gingivalis. , 1994, Research in microbiology.

[40]  Y. Takahashi,et al.  Genetic transformation of Porphyromonas gingivalis by electroporation. , 1993, Oral microbiology and immunology.

[41]  H Löe,et al.  Prevalence, extent, severity and progression of periodontal disease. , 1993, Periodontology 2000.

[42]  J. M. Smith,et al.  How clonal are bacteria? , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[43]  T. Whittam,et al.  Genetic structure of populations of Porphyromonas gingivalis associated with periodontitis and other oral infections , 1993, Infection and immunity.

[44]  A. Dress,et al.  Split decomposition: a new and useful approach to phylogenetic analysis of distance data. , 1992, Molecular phylogenetics and evolution.

[45]  R. Brousseau,et al.  Application of polymerase chain reaction with arbitrary primer (AP-PCR) to strain identification of Porphyromonas (Bacteroides) gingivalis. , 1992, FEMS microbiology letters.

[46]  T. Kato,et al.  Sequence analysis and characterization of the Porphyromonas gingivalis prtC gene, which expresses a novel collagenase activity , 1992, Journal of bacteriology.

[47]  A. Sheiham.  Public health aspects of periodontal diseases in Europe. , 1991, Journal of clinical periodontology.

[48]  R. Genco,et al.  Genetic Heterogeneity of Porphyromonas (Bacteroides) gingivalis by Genomic DNA Fingerprinting , 1990, Journal of dental research.

[49]  V. Baelum,et al.  Periodontal diseases in adult Kenyans. , 1988, Journal of clinical periodontology.

[50]  T. Pilot,et al.  An update on periodontal conditions in adults, measured by CPITN. , 1987, International dental journal.

[51]  J. Stephens,et al.  Statistical methods of DNA sequence analysis: detection of intragenic recombination or gene conversion. , 1985, Molecular biology and evolution.