Immunohistochemical study of intimal microvessels in coronary atherosclerosis.

Two hundred ninety-nine human coronary artery paraffin-embedded tissue blocks were examined for intimal microvessel invasion by probing for factor VIII-associated antigen with indirect immunofluorescence and high resolution confocal microscopy. The results obtained confirm that intimal microvessels originate in the adventitia and show that the richness of intimal microvessels is strongly positively correlated with intimal thickness and negatively correlated with relative lumen size. A number of plasma constituents were examined in serial sections. Comparison of immunofluorescence distribution patterns of these components with intimal microvessel distribution patterns reveals that intimal microvessels leak plasma albumin into artery walls, exude fibrinogen, and are associated with the build-up of plasma cells within atherosclerotic lesions. Therefore, intimal microvessels are demonstrated to play important roles in the development of atherosclerosis.

[1]  S. Parthasarathy,et al.  Macrophage-derived foam cells freshly isolated from rabbit atherosclerotic lesions degrade modified lipoproteins, promote oxidation of low-density lipoproteins, and contain oxidation-specific lipid-protein adducts. , 1991, The Journal of clinical investigation.

[2]  A. Barger,et al.  Rupture of coronary vasa vasorum as a trigger of acute myocardial infarction. , 1990, The American journal of cardiology.

[3]  M. Davies,et al.  Post‐mortem investigation and quantification of coronary artery disease , 1985, Histopathology.

[4]  A. Barger,et al.  Hypothesis: vasa vasorum and neovascularization of human coronary arteries. A possible role in the pathophysiology of atherosclerosis. , 1984, The New England journal of medicine.

[5]  G. Schoefl,et al.  Pathological vascularization of the coronary intima. , 1983, Ciba Foundation symposium.

[6]  S. Grundy,et al.  The possible role of the arterial microcirculation in the pathogenesis of atherosclerosis. , 1980, Journal of chronic diseases.

[7]  J. Kadish.  Fibrin and atherogenesis--a hypothesis. , 1979, Atherosclerosis.

[8]  Reddy Gs,et al.  Morphologic changes in arterial grafts in rabbit ears. , 1979 .

[9]  W. J. Cliff,et al.  Morphologic changes in arterial grafts in rabbit ears. , 1979, Laboratory investigation; a journal of technical methods and pathology.

[10]  S. Huang,et al.  Application of immunofluorescent staining on paraffin sections improved by trypsin digestion. , 1976, Laboratory investigation; a journal of technical methods and pathology.

[11]  L. Hoyer,et al.  Antihemophilic factor antigen. Localization in endothelial cells by immunofluorescent microscopy. , 1973, The Journal of clinical investigation.

[12]  V. Vancov.  Structural basis of the microcirculation in the wall of arterial vessels. , 1973, Bibliotheca anatomica.

[13]  N. Pedersen,et al.  THE ROLE OF THE LYMPHATIC SYSTEM IN THE REJECTION OF HOMOGRAFTS: A STUDY OF LYMPH FROM RENAL TRANSPLANTS , 1970, The Journal of experimental medicine.

[14]  G. S. Boyd,et al.  Atherosclerosis: Pathology, physiology, aetiology, diagnosis and clinical management , 1969 .

[15]  H. Blumenthal.  Cowdry's arteriosclerosis : a survey of the problem , 1967 .

[16]  G. Hayward.  The Incubation Period of Coronary Thrombosis , 1963 .

[17]  G. Schoefl,et al.  STUDIES ON INFLAMMATION. III. GROWING CAPILLARIES: THEIR STRUCTURE AND PERMEABILITY. , 1963, Virchows Archiv fur pathologische Anatomie und Physiologie und fur klinische Medizin.

[18]  W. Cliff.  Observations on healing tissue: A combined light and electron microscopic investigation , 1963, Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences.

[19]  M. Friedman,et al.  Origin of lipid and cholesterol in experimental thromboatherosclerosis. , 1962, The Journal of clinical investigation.

[20]  H. Schornagel.  Intimal thickening in the coronary arteries in infants. , 1956, A.M.A. archives of pathology.

[21]  E. Geiringer.  Intimal vascularization and atherosclerosis. , 1951, The Journal of pathology and bacteriology.

[22]  T. Leary.  Vascularization of atherosclerotic lesions , 1938 .

[23]  Alwin M. Pappenheimer,et al.  The Biology of Arteriosclerosis , 1938, The Yale Journal of Biology and Medicine.

[24]  M. J. Schlesinger,et al.  An injection plus dissection study of coronary artery occlusions and anastomoses , 1938 .