A three-dimensional model of fungal morphogenesis based on the vesicle supply center concept.

We developed a three-dimensional model of hyphal morphogenesis under the same basic assumption used for the construction of a two-dimensional model. Namely, that the polarized growth of tubular cells (hyphoids) arises from a gradient of wall-building vesicles generated by a vesicle supply center (VSC). Contrary to the 2-D mathematical formulation, the three-dimensional derivation led to an indetermination whose solution required defining a priori the pattern of expansion of the wall, i.e. defining the overall spatial movement of the wall as the newly inserted wall elements displace the existing wall fabric. The patterns of wall expansion can be described by tracing the movement of marker points on the cell surface (point trajectories). Point trajectories were computed for three different modes of wall expansion of the VSC-generated hyphoids: orthogonal, isometric, and rotational. The 3-D VSC models allowed us to either stipulate or calculate the degree of anisotropy for each type of wall expansion. Wireframe models were built to visualize growth anisotropy in each model. Although the overall shape of the three hyphoid models is similar, they differ substantially in point trajectories and anisotropy. Point trajectories are experimentally testable and were the basis for the conclusion that hyphae grow in orthogonal fashion. (Bartnicki-Garcia et al., 2000. Biophys. J.79, 2382-2390.)

[1]  Allen L. King,et al.  A Mechanism for the Origin of Specifically Oriented Textures in Development with Special Reference to Nitella Wall Texture , 1966 .

[2]  Computer modelling of hyphal tip growth in fungi , 1972 .

[3]  C. F. Fox,et al.  Biochemistry of cell walls and membranes , 1975 .

[4]  P. T. Saunders,et al.  Tip Growth of Fungal Hyphae , 1977 .

[5]  A P Trinci,et al.  A model for hyphal growth and branching. , 1979, Journal of general microbiology.

[6]  A. L. Koch The Shape of the Hyphal Tips of Fungi , 1982 .

[7]  S. Bartnicki-García 8 – Role of Vesicles in Apical Growth and a New Mathematical Model of Hyphal Morphogenesis , 1990 .

[8]  G. Gierz,et al.  A Novel Computer Model for Generating Cell Shape: Application to Fungal Morphogenesis , 1990 .

[9]  I. Heath Tip growth in plant and fungal cells , 1992 .

[10]  D. Kerridge,et al.  Dimorphic Fungi in Biology and Medicine , 2012, Springer US.

[11]  G. Gierz,et al.  Mathematical Analysis of the Cellular Basis of Fungal Dimorphism , 1993 .

[12]  A. L. Koch The Problem of Hyphal Growth in Streptomycetes and Fungi , 1994 .

[13]  Determinants of fungal cell wall morphology: the vesicle supply center , 1995 .

[14]  J. Prosser Mathematical Modelling of Fungal Growth , 1995 .

[15]  G. Gadd,et al.  The Growing Fungus , 1995, Springer Netherlands.

[16]  G. Gierz,et al.  Evidence that Spitzenkörper behavior determines the shape of a fungal hypha: a test of the hyphoid model. , 1995, Experimental mycology.

[17]  N. P. Money Wishful Thinking of Turgor Revisited: The Mechanics of Fungal Growth , 1997 .

[18]  C. G. Reynaga-Peña,et al.  What determines growth direction in fungal hyphae? , 1998, Fungal genetics and biology : FG & B.

[19]  G. Steinberg,et al.  Mechanisms of hyphal tip growth: tube dwelling amebae revisited. , 1999, Fungal genetics and biology : FG & B.

[20]  G. Gierz,et al.  Mapping the growth of fungal hyphae: orthogonal cell wall expansion during tip growth and the role of turgor. , 2000, Biophysical journal.