论文信息 - SMB Operation for Three-Fraction Separations: Purification of Plasmid DNA

SMB Operation for Three-Fraction Separations: Purification of Plasmid DNA

The Simulated Moving Bed technology is extended to incorporate a cleaning in place step, and it is then applied by exploiting size exclusion chromatography to purify plasmid DNA. Experimental performances are discussed in the light of our theoretical understanding of the SMB behavior.

[1] Massimo Morbidelli,et al. Optimal operation of simulated moving bed units for nonlinear chromatographic separations , 1997 .

[2] Massimo Morbidelli,et al. Simulated moving‐bed units with extra‐column dead volume , 1999 .

[3] P Dunnill,et al. Biochemical engineering approaches to the challenges of producing pure plasmid DNA. , 2000, Trends in biotechnology.

[4] D. Prazeres,et al. Downstream processing of plasmid DNA for gene therapy and DNA vaccine applications. , 2000, Trends in biotechnology.

[5] Sabine Imamoglu,et al. Simulated moving bed chromatography (SMB) for application in bioseparation. , 2002, Advances in biochemical engineering/biotechnology.

[6] P. Shamlou,et al. Scaleable processes for the manufacture of therapeutic quantities of plasmid DNA , 2003, Biotechnology and applied biochemistry.

[7] M. Morbidelli,et al. Simulated moving-bed chromatography and its application to chirotechnology. , 2000, Trends in biotechnology.

[8] Raf Lemmens,et al. Plasmid DNA purification , 2004 .

[9] Cordin Arpagaus,et al. Two-fraction and three-fraction continuous simulated moving bed separation of nucleosides. , 2004, Journal of chromatography. A.