Harmonic analysis of electron microscope images with rotational symmetry.

Abstract This paper describes a method of analysing images from electron micrographs of biological specimens believed to possess rotational symmetry. An objective analysis of the symmetry is possible because the method, which is computational, produces a rotational power spectrum of the image. We can then combine just those components which are consistent with the previously determined symmetry to produce a filtered image. The method is applied to the base plate of bacteriophage T4 and to discs of tobacco mosaic virus protein. The advantages of this new approach over the well-known Markham rotation technique are discussed.

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