Comparison of three ELISA kits for the detection of antibodies against foot-and-mouth disease virus non-structural proteins

Three foot-and-mouth disease virus non-structural protein antibody kits: CHEKIT FMD-3ABC, Ceditest FMDVNS and 3ABC-ELISA were compared. These ELISAs were performed using panel of sera collected from negative (noninfected), vaccinated with trivalent foot and mouth disease vaccine, experimentally infected cattle as well as from animals that had been vaccinated and subsequently infected. Moreover, the panel of the FAO/OIE international reference sera for the purposes of Phase XVIII exercise was tested. The Ceditest kit had a better relative specificity (99.7% for naive and 98.8% for vaccinated cattle) than the CHEKIT and 3ABC-ELISA kits (97.2 – 98.8% and 98.1 – 99.2%, respectively). A relative sensitivity of all used kits exceeded 98%, however, the Ceditest FMDV-NS kit had the higher sensitivity (99.1%) than the CHEKIT and 3ABC-ELISA (98.2%). The weak 2 O SKR 1/2000 reference serum tested within Phase XVIII collaborative study was detected only in Ceditest kit. It can be concluded that all tested kits can be a useful tool for export/import serological examination, for foot and mouth disease control programmes and especially for eradication campaigns in situation where emergency vaccination was

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