Expression of Annexin-A1 and Galectin-1 Anti-Inflammatory Proteins and mRNA in Chronic Gastritis and Gastric Cancer

Objective. The anti-inflammatory proteins annexin-A1 and galectin-1 have been associated with tumor progression. This scenario prompted us to investigate the relationship between the gene and protein expression of annexin-A1 (ANXA1/AnxA1) and galectin-1 (LGALS1/Gal-1) in an inflammatory gastric lesion as chronic gastritis (CG) and gastric adenocarcinoma (GA) and its association with H. pylori infection. Methods. We analyzed 40 samples of CG, 20 of GA, and 10 of normal mucosa (C) by the quantitative real-time PCR (qPCR) technique and the immunohistochemistry assay. Results. High ANXA1 mRNA expression levels were observed in 90% (36/40) of CG cases (mean relative quantification RQ = 4.26  ±  2.03) and in 80% (16/20) of GA cases (mean RQ = 4.38  ±  4.77). However, LGALS1 mRNA levels were high (mean RQ = 2.44  ±  3.26) in 60% (12/20) of the GA cases, while low expression was found in CG (mean RQ = 0.43 ± 3.13; P < 0.01). Normal mucosa showed modest immunoreactivity in stroma but not in epithelium, while stroma and epithelium displayed an intense immunostaining in CG and GA for both proteins. Conclusion. These results have provided evidence that galectin-1 and mainly annexin-A1 are overexpressed in both gastritis and gastric cancer, suggesting a strong association of these proteins with chronic gastric inflammation and carcinogenesis.

[1]  Ming-Tsan Lin,et al.  Annexin A1 is associated with gastric cancer survival and promotes gastric cancer cell invasiveness through the formyl peptide receptor/extracellular signal‐regulated kinase/integrin beta‐1‐binding protein 1 pathway , 2012, Cancer.

[2]  T. Ushijima,et al.  Inflammation-associated cancer development in digestive organs: mechanisms and roles for genetic and epigenetic modulation. , 2012, Gastroenterology.

[3]  H. Juan,et al.  Helicobacter pylori Disrupts Host Cell Membranes, Initiating a Repair Response and Cell Proliferation , 2012, International journal of molecular sciences.

[4]  A. Karkhane,et al.  Proteomics of a new esophageal cancer cell line established from Persian patient. , 2012, Gene.

[5]  C. Chai,et al.  Overexpression of annexin 1 in the development and differentiation of urothelial carcinoma , 2012, The Kaohsiung journal of medical sciences.

[6]  S. Lei,et al.  Tissue and serum galectin-1 expression in patients with colorectal carcinoma. , 2012, Hepato-gastroenterology.

[7]  D. Noh,et al.  Clinical Significance of Annexin A1 Expression in Breast Cancer , 2011, Journal of breast cancer.

[8]  P. Kamper,et al.  Proteomic analysis identifies galectin-1 as a predictive biomarker for relapsed/refractory disease in classical Hodgkin lymphoma. , 2011, Blood.

[9]  D. Kang,et al.  Oncogenic CagA Promotes Gastric Cancer Risk via Activating ERK Signaling Pathways: A Nested Case-Control Study , 2011, PloS one.

[10]  S. Zucoloto,et al.  Myenteric Denervation Downregulates Galectin-1 and -3 Expression in Gastric Carcinogenesis , 2011, Digestive Diseases and Sciences.

[11]  Yu Li,et al.  Human gastrin mRNA expression up-regulated by Helicobacter pylori CagA through MEK/ERK and JAK2-signaling pathways in gastric cancer cells , 2011, Gastric Cancer.

[12]  Peng-Fei Zhang,et al.  Identification of annexin A1 as a proinvasive and prognostic factor for lung adenocarcinoma , 2011, Clinical & Experimental Metastasis.

[13]  K. Leite,et al.  Expression of TERT in precancerous gastric lesions compared to gastric cancer. , 2011, Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas.

[14]  X. Xu,et al.  Increased expression of annexin A1 is correlated with K-ras mutation in colorectal cancer. , 2010, The Tohoku journal of experimental medicine.

[15]  S. Zeng,et al.  Nuclear Localization of Annexin A1 Correlates With Advanced Disease and Peritoneal Dissemination in Patients with Gastric Carcinoma , 2010, Anatomical record.

[16]  S. Bektaş,et al.  CD24 and Galectin-1 Expressions in Gastric Adenocarcinoma and Clinicopathologic Significance , 2010, Pathology & Oncology Research.

[17]  S. Rogatto,et al.  Quantitative real-time RT-PCR and chromogenic in situ hybridization: precise methods to detect HER-2 status in breast carcinoma , 2009, BMC Cancer.

[18]  T. Putti,et al.  Annexin-1 Regulates Growth Arrest Induced by High Levels of Estrogen in MCF-7 Breast Cancer Cells , 2009, Molecular Cancer Research.

[19]  J. Buckingham,et al.  Annexin A1 and the formyl peptide receptor family: neuroendocrine and metabolic aspects. , 2008, Current opinion in pharmacology.

[20]  K. Murakami,et al.  Helicobacter pylori virulence and the diversity of gastric cancer in Asia. , 2008, Journal of medical microbiology.

[21]  C. Scapulatempo-Neto,et al.  Annexin A1 subcellular expression in laryngeal squamous cell carcinoma , 2008, Histopathology.

[22]  Qiang Li,et al.  Tissue microarray analysis reveals strong clinical evidence for a close association between loss of annexin A1 expression and nodal metastasis in gastric cancer , 2008, Clinical & Experimental Metastasis.

[23]  Hui Jiang,et al.  How is mRNA expression predictive for protein expression? A correlation study on human circulating monocytes. , 2008, Acta biochimica et biophysica Sinica.

[24]  Y. Jeng,et al.  Nuclear localization of annexin A1 is a prognostic factor in oral squamous cell carcinoma , 2008, Journal of surgical oncology.

[25]  H. Juan,et al.  Annexin A4: A novel molecular marker for gastric cancer with Helicobacter pylori infection using proteomics approach , 2008, Proteomics. Clinical applications.

[26]  J. Wallace,et al.  Annexin-1 modulates repair of gastric mucosal injury. , 2008, American journal of physiology. Gastrointestinal and liver physiology.

[27]  C. Decaestecker,et al.  Increased expression and altered intracellular distribution of adhesion/growth‐regulatory lectins galectins‐1 and ‐7 during tumour progression in hypopharyngeal and laryngeal squamous cell carcinomas , 2008, Histopathology.

[28]  E. Tajara,et al.  Annexin 1: Differential expression in tumor and mast cells in human larynx cancer , 2007, International journal of cancer.

[29]  U. Rescher,et al.  Annexins as intracellular calcium sensors. , 2007, Cell calcium.

[30]  P. Cury,et al.  Spatial expression of two anti‐inflammatory mediators, annexin 1 and galectin‐1, in nasal polyposis , 2006, Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology.

[31]  Hsuan-Cheng Huang,et al.  Quantitative proteomic and genomic profiling reveals metastasis-related protein expression patterns in gastric cancer cells. , 2006, Journal of proteome research.

[32]  Marília Cardoso Smith,et al.  CagA positive Helicobacter pylori in Brazilian children related to chronic gastritis. , 2006, The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases.

[33]  E. Kuipers,et al.  Pathogenesis of Helicobacter pylori Infection , 2006, Clinical Microbiology Reviews.

[34]  M. Perretti,et al.  Inflammation-induced modulation of cellular galectin-1 and -3 expression in a model of rat peritonitis , 2006, Inflammation Research.

[35]  M. Heineman,et al.  Sex hormones and the immune response in humans. , 2005, Human reproduction update.

[36]  H. Isomoto,et al.  The Relationship between Plasma and Gastric Ghrelin Levels and Strain Diversity in Helicobacter pylori Virulence. , 2005, The American journal of gastroenterology.

[37]  S. Moss,et al.  Annexins: linking Ca2+ signalling to membrane dynamics , 2005, Nature Reviews Molecular Cell Biology.

[38]  G. Rabinovich.  Galectin-1 as a potential cancer target , 2005, British Journal of Cancer.

[39]  P. Matarrese,et al.  Galectin-1 Sensitizes Resting Human T Lymphocytes to Fas (CD95)-mediated Cell Death via Mitochondrial Hyperpolarization, Budding, and Fission* , 2005, Journal of Biological Chemistry.

[40]  T. Azuma,et al.  Meta-analysis of the relationship between CagA seropositivity and gastric cancer. , 2004, Gastroenterology.

[41]  E. Solito,et al.  Annexin 1: more than an anti-phospholipase protein , 2004, Inflammation Research.

[42]  M. Gerstein,et al.  Comparing protein abundance and mRNA expression levels on a genomic scale , 2003, Genome Biology.

[43]  Hyeyoung Kim,et al.  Cell adhesion-related gene expression by Helicobacter pylori in gastric epithelial AGS cells. , 2003, The international journal of biochemistry & cell biology.

[44]  Xiaohang Zhao,et al.  Translocation of annexin I from cellular membrane to the nuclear membrane in human esophageal squamous cell carcinoma. , 2003, World journal of gastroenterology.

[45]  Megumi Higuchi,et al.  Attenuation of Helicobacter pylori CagA·SHP-2 Signaling by Interaction between CagA and C-terminal Src Kinase* , 2003, The Journal of Biological Chemistry.

[46]  C. Prinz,et al.  Pathogenesis of Helicobacter pylori infection , 2002, Helicobacter.

[47]  David E. Misek,et al.  Discordant Protein and mRNA Expression in Lung Adenocarcinomas * , 2002, Molecular & Cellular Proteomics.

[48]  M. Pfaffl,et al.  A new mathematical model for relative quantification in real-time RT-PCR. , 2001, Nucleic acids research.

[49]  J. Jen,et al.  Alcohol consumption and cigarette smoking increase the frequency of p53 mutations in non-small cell lung cancer. , 2000, Cancer research.

[50]  D. Beall.  Classification and Grading of Gastritis: The Updated Sydney System , 1997 .

[51]  M F Dixon,et al.  Classification and grading of gastritis. The updated Sydney System. International Workshop on the Histopathology of Gastritis, Houston 1994. , 1996, The American journal of surgical pathology.

[52]  P. Correa,et al.  A human model of gastric carcinogenesis. , 1988, Cancer research.

[53]  P. Laurén,et al.  THE TWO HISTOLOGICAL MAIN TYPES OF GASTRIC CARCINOMA: DIFFUSE AND SO-CALLED INTESTINAL-TYPE CARCINOMA. AN ATTEMPT AT A HISTO-CLINICAL CLASSIFICATION. , 1965, Acta pathologica et microbiologica Scandinavica.

[54]  J. Barkin,et al.  Stomach , 2015, The American Journal of Gastroenterology.

[55]  M. Fresno,et al.  Down-regulation of annexin A1 and A2 protein expression in intestinal-type sinonasal adenocarcinomas. , 2011, Human pathology.

[56]  M. Perretti,et al.  Functional and ultrastructural analysis of annexin A1 and its receptor in extravasating neutrophils during acute inflammation. , 2009, The American journal of pathology.

[57]  Li‐yu Lee,et al.  Identification of differential gene expression between intestinal and diffuse gastric cancer using cDNA microarray. , 2006, Oncology reports.

[58]  A. Karlsson,et al.  Galectins as inflammatory mediators , 2004, Glycoconjugate Journal.

[59]  M. Blaser,et al.  Helicobacter pylori and gastrointestinal tract adenocarcinomas , 2002, Nature Reviews Cancer.

[60]  D. Ornstein,et al.  Dysregulation of annexin I protein expression in high-grade prostatic intraepithelial neoplasia and prostate cancer. , 2002, Clinical cancer research : an official journal of the American Association for Cancer Research.